Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2823 and GFF2824 are separated by 130 nucleotides GFF2824 and GFF2825 are separated by 5 nucleotides GFF2825 and GFF2826 are separated by 80 nucleotides
GFF2823 - DinG family ATP-dependent helicase YoaA, at 343,787 to 345,697
GFF2823
GFF2824 - RidA/YER057c/UK114 superfamily, group 2, YoaB-like protein, at 345,828 to 346,172
GFF2824
GFF2825 - FIG00638134: hypothetical protein, at 346,178 to 346,357
GFF2825
GFF2826 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 346,438 to 347,802
GFF2826
Position (kb) 346
347 Strain fitness (log2 ratio) -1
0
1
2 at 345.305 kb on + strand, within GFF2823 at 345.333 kb on - strand, within GFF2823 at 345.534 kb on + strand at 345.535 kb on - strand at 345.535 kb on - strand at 345.540 kb on + strand at 345.540 kb on + strand at 345.541 kb on - strand at 345.726 kb on + strand at 345.727 kb on - strand at 345.746 kb on + strand at 345.822 kb on + strand at 345.822 kb on + strand at 345.823 kb on - strand at 345.826 kb on + strand at 345.826 kb on + strand at 345.827 kb on - strand at 345.880 kb on - strand, within GFF2824 at 345.884 kb on + strand, within GFF2824 at 346.112 kb on - strand, within GFF2824 at 346.276 kb on + strand, within GFF2825 at 346.276 kb on + strand, within GFF2825 at 346.362 kb on - strand at 346.372 kb on - strand at 346.390 kb on - strand at 346.417 kb on + strand at 346.638 kb on - strand, within GFF2826 at 346.641 kb on + strand, within GFF2826 at 346.782 kb on - strand, within GFF2826 at 346.810 kb on - strand, within GFF2826 at 346.909 kb on + strand, within GFF2826 at 347.265 kb on + strand, within GFF2826
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 345,305 + GFF2823 0.79 -0.3 345,333 - GFF2823 0.81 -0.2 345,534 + -0.2 345,535 - +1.0 345,535 - -0.1 345,540 + +0.5 345,540 + +1.2 345,541 - +0.5 345,726 + +0.2 345,727 - +0.2 345,746 + -0.1 345,822 + +0.0 345,822 + -0.1 345,823 - -0.6 345,826 + +0.2 345,826 + -0.4 345,827 - -0.1 345,880 - GFF2824 0.15 -0.6 345,884 + GFF2824 0.16 -1.0 346,112 - GFF2824 0.82 +1.0 346,276 + GFF2825 0.54 -0.1 346,276 + GFF2825 0.54 -1.2 346,362 - +1.7 346,372 - -0.3 346,390 - -0.0 346,417 + +0.7 346,638 - GFF2826 0.15 -0.1 346,641 + GFF2826 0.15 -0.4 346,782 - GFF2826 0.25 +1.8 346,810 - GFF2826 0.27 +0.2 346,909 + GFF2826 0.35 +1.0 347,265 + GFF2826 0.61 +0.6
Or see this region's nucleotide sequence