Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2672 and GFF2673 are separated by 273 nucleotides GFF2673 and GFF2674 are separated by 118 nucleotides
GFF2672 - oxidoreductase, aldo/keto reductase family, at 194,422 to 195,291
GFF2672
GFF2673 - Probable transcription regulator protein of MDR efflux pump cluster, at 195,565 to 196,470
GFF2673
GFF2674 - FIG00553873: hypothetical protein, at 196,589 to 197,518
GFF2674
Position (kb) 195
196
197 Strain fitness (log2 ratio) -2
-1
0
1
2 at 194.704 kb on - strand, within GFF2672 at 194.750 kb on + strand, within GFF2672 at 194.789 kb on + strand, within GFF2672 at 194.824 kb on - strand, within GFF2672 at 195.037 kb on - strand, within GFF2672 at 195.070 kb on - strand, within GFF2672 at 195.074 kb on - strand, within GFF2672 at 195.271 kb on - strand at 195.275 kb on - strand at 195.327 kb on - strand at 195.327 kb on - strand at 195.369 kb on - strand at 195.390 kb on + strand at 195.422 kb on + strand at 195.440 kb on + strand at 195.441 kb on - strand at 195.455 kb on + strand at 195.737 kb on + strand, within GFF2673 at 195.770 kb on - strand, within GFF2673 at 195.785 kb on + strand, within GFF2673 at 195.956 kb on + strand, within GFF2673 at 196.069 kb on - strand, within GFF2673 at 196.072 kb on - strand, within GFF2673 at 196.189 kb on - strand, within GFF2673 at 196.274 kb on + strand, within GFF2673 at 196.277 kb on + strand, within GFF2673 at 196.360 kb on - strand, within GFF2673 at 196.549 kb on + strand at 196.560 kb on + strand at 196.560 kb on + strand at 196.560 kb on + strand at 196.874 kb on - strand, within GFF2674 at 197.086 kb on - strand, within GFF2674 at 197.090 kb on - strand, within GFF2674 at 197.329 kb on + strand, within GFF2674 at 197.341 kb on - strand, within GFF2674 at 197.432 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 194,704 - GFF2672 0.32 -1.0 194,750 + GFF2672 0.38 -0.5 194,789 + GFF2672 0.42 +0.9 194,824 - GFF2672 0.46 -0.2 195,037 - GFF2672 0.71 -0.5 195,070 - GFF2672 0.74 +0.1 195,074 - GFF2672 0.75 +1.1 195,271 - +0.4 195,275 - -1.8 195,327 - -0.2 195,327 - +0.4 195,369 - +0.1 195,390 + -0.3 195,422 + +0.4 195,440 + -0.9 195,441 - +0.7 195,455 + +0.3 195,737 + GFF2673 0.19 -0.5 195,770 - GFF2673 0.23 -0.4 195,785 + GFF2673 0.24 -0.2 195,956 + GFF2673 0.43 +0.5 196,069 - GFF2673 0.56 +0.1 196,072 - GFF2673 0.56 -0.7 196,189 - GFF2673 0.69 -0.3 196,274 + GFF2673 0.78 +0.5 196,277 + GFF2673 0.79 +0.1 196,360 - GFF2673 0.88 -0.9 196,549 + -0.0 196,560 + +1.9 196,560 + -0.1 196,560 + -0.0 196,874 - GFF2674 0.31 +0.5 197,086 - GFF2674 0.53 -0.2 197,090 - GFF2674 0.54 +1.0 197,329 + GFF2674 0.80 -0.6 197,341 - GFF2674 0.81 -0.2 197,432 - -1.0
Or see this region's nucleotide sequence