Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2395 and GFF2396 are separated by 4 nucleotides GFF2396 and GFF2397 are separated by 4 nucleotides GFF2397 and GFF2398 overlap by 4 nucleotides GFF2398 and GFF2399 overlap by 25 nucleotides GFF2399 and GFF2400 are separated by 44 nucleotides GFF2400 and GFF2401 are separated by 13 nucleotides
GFF2395 - Similar to C-terminal Zn-finger domain of DNA topoisomerase I, at 23,156 to 23,698
GFF2395
GFF2396 - TsaC protein (YrdC domain) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA, at 23,703 to 24,275
GFF2396
GFF2397 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 24,280 to 25,098
GFF2397
GFF2398 - FIG00640156: hypothetical protein, at 25,095 to 25,352
GFF2398
GFF2399 - carbonic anhydrase, family 3, at 25,328 to 25,882
GFF2399
GFF2400 - hypothetical protein, at 25,927 to 26,043
GFF2400
GFF2401 - hypothetical protein, at 26,057 to 26,179
GFF2401
Position (kb) 24
25
26 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 23.432 kb on - strand, within GFF2395 at 23.432 kb on - strand, within GFF2395 at 23.432 kb on - strand, within GFF2395 at 24.273 kb on + strand at 24.277 kb on - strand at 24.277 kb on - strand at 24.277 kb on - strand at 24.277 kb on - strand at 24.289 kb on + strand at 24.289 kb on + strand at 24.290 kb on - strand at 24.333 kb on + strand at 24.333 kb on + strand at 24.363 kb on + strand, within GFF2397 at 24.364 kb on - strand, within GFF2397 at 24.364 kb on - strand, within GFF2397 at 24.364 kb on - strand, within GFF2397 at 24.377 kb on - strand, within GFF2397 at 24.537 kb on + strand, within GFF2397 at 24.579 kb on + strand, within GFF2397 at 24.580 kb on - strand, within GFF2397 at 24.717 kb on + strand, within GFF2397 at 24.795 kb on + strand, within GFF2397 at 24.828 kb on - strand, within GFF2397 at 24.888 kb on + strand, within GFF2397 at 24.889 kb on - strand, within GFF2397 at 24.910 kb on + strand, within GFF2397 at 24.911 kb on - strand, within GFF2397 at 25.043 kb on + strand at 25.043 kb on + strand at 25.047 kb on - strand at 25.077 kb on - strand at 25.088 kb on + strand at 25.152 kb on - strand, within GFF2398 at 25.359 kb on + strand at 25.376 kb on + strand at 25.377 kb on - strand at 25.380 kb on + strand at 25.381 kb on - strand at 25.461 kb on - strand, within GFF2399 at 25.518 kb on - strand, within GFF2399 at 25.529 kb on + strand, within GFF2399 at 25.529 kb on + strand, within GFF2399 at 25.603 kb on + strand, within GFF2399 at 25.603 kb on + strand, within GFF2399 at 25.679 kb on + strand, within GFF2399 at 25.712 kb on - strand, within GFF2399 at 25.732 kb on - strand, within GFF2399 at 25.858 kb on + strand at 25.859 kb on - strand at 25.859 kb on - strand at 25.859 kb on - strand at 25.860 kb on + strand at 25.868 kb on + strand at 25.868 kb on + strand at 25.869 kb on - strand at 25.869 kb on - strand at 25.886 kb on + strand at 25.887 kb on - strand at 25.896 kb on + strand at 25.904 kb on + strand at 25.905 kb on - strand at 25.952 kb on - strand, within GFF2400 at 25.952 kb on - strand, within GFF2400
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 23,432 - GFF2395 0.51 +1.6 23,432 - GFF2395 0.51 +0.1 23,432 - GFF2395 0.51 -1.3 24,273 + -0.3 24,277 - +1.0 24,277 - +0.6 24,277 - +0.2 24,277 - +0.6 24,289 + -0.2 24,289 + +0.4 24,290 - -1.4 24,333 + -0.4 24,333 + -2.8 24,363 + GFF2397 0.10 -1.3 24,364 - GFF2397 0.10 -1.0 24,364 - GFF2397 0.10 -1.5 24,364 - GFF2397 0.10 -1.6 24,377 - GFF2397 0.12 -0.9 24,537 + GFF2397 0.31 -0.9 24,579 + GFF2397 0.37 -1.0 24,580 - GFF2397 0.37 +0.1 24,717 + GFF2397 0.53 -0.7 24,795 + GFF2397 0.63 -0.9 24,828 - GFF2397 0.67 +0.7 24,888 + GFF2397 0.74 -0.5 24,889 - GFF2397 0.74 +0.1 24,910 + GFF2397 0.77 -1.2 24,911 - GFF2397 0.77 +0.3 25,043 + -0.7 25,043 + -1.4 25,047 - -0.3 25,077 - +0.1 25,088 + +0.5 25,152 - GFF2398 0.22 +0.1 25,359 + -0.0 25,376 + +0.1 25,377 - -0.0 25,380 + +0.2 25,381 - -0.4 25,461 - GFF2399 0.24 -0.3 25,518 - GFF2399 0.34 +0.0 25,529 + GFF2399 0.36 -0.1 25,529 + GFF2399 0.36 -0.8 25,603 + GFF2399 0.50 +0.8 25,603 + GFF2399 0.50 -0.2 25,679 + GFF2399 0.63 -0.9 25,712 - GFF2399 0.69 +0.4 25,732 - GFF2399 0.73 -0.3 25,858 + -0.1 25,859 - -0.0 25,859 - -0.2 25,859 - -0.5 25,860 + -0.0 25,868 + +1.5 25,868 + -0.3 25,869 - +1.1 25,869 - -0.3 25,886 + -0.2 25,887 - +0.0 25,896 + -0.1 25,904 + +1.2 25,905 - +0.1 25,952 - GFF2400 0.21 +0.8 25,952 - GFF2400 0.21 +0.5
Or see this region's nucleotide sequence