Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1293 and GFF1294 are separated by 9 nucleotides GFF1294 and GFF1295 are separated by 108 nucleotides GFF1295 and GFF1296 are separated by 38 nucleotides
GFF1293 - Phage minor tail protein, at 18,031 to 18,360
GFF1293
GFF1294 - Phage minor tail protein, at 18,370 to 19,068
GFF1294
GFF1295 - Phage tail assembly protein, at 19,177 to 19,812
GFF1295
GFF1296 - Phage tail assembly protein I, at 19,851 to 20,357
GFF1296
Position (kb) 19
20 Strain fitness (log2 ratio) -1
0
1 at 18.183 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.261 kb on + strand, within GFF1293 at 18.391 kb on - strand at 18.536 kb on - strand, within GFF1294 at 18.689 kb on + strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.693 kb on - strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.694 kb on + strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.695 kb on - strand, within GFF1294 at 18.807 kb on - strand, within GFF1294 at 18.888 kb on + strand, within GFF1294 at 18.896 kb on + strand, within GFF1294 at 18.919 kb on + strand, within GFF1294 at 18.921 kb on + strand, within GFF1294 at 18.957 kb on + strand, within GFF1294 at 19.054 kb on - strand at 19.089 kb on + strand at 19.166 kb on - strand at 19.182 kb on + strand at 19.204 kb on + strand at 19.409 kb on - strand, within GFF1295 at 19.433 kb on - strand, within GFF1295 at 19.453 kb on + strand, within GFF1295 at 19.454 kb on - strand, within GFF1295 at 19.555 kb on + strand, within GFF1295 at 19.700 kb on - strand, within GFF1295 at 19.700 kb on - strand, within GFF1295 at 19.811 kb on - strand at 19.894 kb on + strand at 19.894 kb on + strand at 19.901 kb on - strand at 19.977 kb on - strand, within GFF1296 at 20.100 kb on - strand, within GFF1296 at 20.103 kb on + strand, within GFF1296 at 20.103 kb on + strand, within GFF1296 at 20.104 kb on - strand, within GFF1296 at 20.104 kb on - strand, within GFF1296 at 20.104 kb on - strand, within GFF1296 at 20.104 kb on - strand, within GFF1296 at 20.106 kb on - strand, within GFF1296 at 20.106 kb on - strand, within GFF1296 at 20.208 kb on + strand, within GFF1296 at 20.416 kb on + strand at 20.618 kb on + strand at 20.619 kb on - strand at 20.620 kb on + strand at 20.693 kb on + strand at 20.704 kb on + strand at 20.760 kb on + strand at 20.761 kb on - strand at 20.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 18,183 + GFF1293 0.46 -0.2 18,261 + GFF1293 0.70 -0.3 18,261 + GFF1293 0.70 +0.6 18,261 + GFF1293 0.70 -0.1 18,391 - -0.5 18,536 - GFF1294 0.24 +0.2 18,689 + GFF1294 0.46 -1.1 18,693 - GFF1294 0.46 +0.2 18,693 - GFF1294 0.46 -0.7 18,693 - GFF1294 0.46 +0.4 18,694 + GFF1294 0.46 -0.2 18,694 + GFF1294 0.46 +0.2 18,694 + GFF1294 0.46 -0.9 18,695 - GFF1294 0.46 +0.1 18,695 - GFF1294 0.46 -0.6 18,695 - GFF1294 0.46 -0.1 18,807 - GFF1294 0.63 -0.2 18,888 + GFF1294 0.74 -0.1 18,896 + GFF1294 0.75 -0.3 18,919 + GFF1294 0.79 +0.5 18,921 + GFF1294 0.79 +0.8 18,957 + GFF1294 0.84 +0.0 19,054 - -1.3 19,089 + +0.1 19,166 - -0.2 19,182 + +0.1 19,204 + -0.1 19,409 - GFF1295 0.36 +0.9 19,433 - GFF1295 0.40 -0.0 19,453 + GFF1295 0.43 +0.3 19,454 - GFF1295 0.44 -0.4 19,555 + GFF1295 0.59 +0.6 19,700 - GFF1295 0.82 +0.7 19,700 - GFF1295 0.82 -0.2 19,811 - +0.0 19,894 + -0.3 19,894 + -0.1 19,901 - -0.2 19,977 - GFF1296 0.25 +0.1 20,100 - GFF1296 0.49 +0.4 20,103 + GFF1296 0.50 -0.2 20,103 + GFF1296 0.50 +0.4 20,104 - GFF1296 0.50 -0.2 20,104 - GFF1296 0.50 +0.3 20,104 - GFF1296 0.50 -0.3 20,104 - GFF1296 0.50 +0.0 20,106 - GFF1296 0.50 +0.2 20,106 - GFF1296 0.50 +0.2 20,208 + GFF1296 0.70 +1.0 20,416 + +0.0 20,618 + +0.6 20,619 - +0.4 20,620 + -0.2 20,693 + +0.5 20,704 + +0.1 20,760 + +0.4 20,761 - -0.7 20,761 - +0.3
Or see this region's nucleotide sequence