Experiment: 2,4-Dinitrophenol 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrdD and treC are separated by 393 nucleotides treC and treB are separated by 49 nucleotides
b4238: nrdD - anaerobic ribonucleoside triphosphate reductase (NCBI), at 4,458,545 to 4,460,683
nrdD
b4239: treC - trehalose-6-P hydrolase (NCBI), at 4,461,077 to 4,462,732
treC
b4240: treB - fused trehalose(maltose)-specific PTS enzyme: IIB component/IIC component (NCBI), at 4,462,782 to 4,464,203
treB
Position (kb)
4461
4462
4463 Strain fitness (log2 ratio)
-2
-1
0
1 at 4460.100 kb on + strand, within nrdD at 4460.168 kb on - strand, within nrdD at 4460.186 kb on + strand, within nrdD at 4460.196 kb on + strand, within nrdD at 4460.196 kb on + strand, within nrdD at 4460.218 kb on + strand, within nrdD at 4460.225 kb on + strand, within nrdD at 4460.225 kb on + strand, within nrdD at 4460.238 kb on - strand, within nrdD at 4460.243 kb on + strand, within nrdD at 4460.260 kb on - strand, within nrdD at 4460.360 kb on - strand, within nrdD at 4460.371 kb on + strand, within nrdD at 4460.371 kb on + strand, within nrdD at 4460.387 kb on + strand, within nrdD at 4460.447 kb on - strand, within nrdD at 4460.447 kb on - strand, within nrdD at 4460.504 kb on - strand at 4460.504 kb on - strand at 4460.542 kb on - strand at 4460.547 kb on - strand at 4460.554 kb on + strand at 4460.661 kb on + strand at 4460.820 kb on + strand at 4460.876 kb on + strand at 4460.891 kb on - strand at 4460.891 kb on - strand at 4461.038 kb on + strand at 4461.038 kb on + strand at 4461.080 kb on + strand at 4461.097 kb on - strand at 4461.128 kb on + strand at 4461.145 kb on + strand at 4461.190 kb on - strand at 4461.295 kb on + strand, within treC at 4461.314 kb on + strand, within treC at 4461.427 kb on + strand, within treC at 4461.533 kb on + strand, within treC at 4461.533 kb on + strand, within treC at 4461.857 kb on - strand, within treC at 4461.957 kb on - strand, within treC at 4461.977 kb on - strand, within treC at 4462.007 kb on + strand, within treC at 4462.145 kb on + strand, within treC at 4462.223 kb on - strand, within treC at 4462.251 kb on + strand, within treC at 4462.328 kb on + strand, within treC at 4462.328 kb on + strand, within treC at 4462.333 kb on + strand, within treC at 4462.358 kb on - strand, within treC at 4462.415 kb on + strand, within treC at 4462.421 kb on + strand, within treC at 4462.421 kb on + strand, within treC at 4462.422 kb on - strand, within treC at 4462.447 kb on - strand, within treC at 4462.447 kb on - strand, within treC at 4462.526 kb on + strand, within treC at 4462.526 kb on + strand, within treC at 4462.613 kb on + strand at 4462.655 kb on + strand at 4462.736 kb on - strand at 4462.742 kb on - strand at 4462.742 kb on - strand at 4462.747 kb on - strand at 4462.810 kb on - strand at 4462.810 kb on - strand at 4462.824 kb on + strand at 4462.840 kb on - strand at 4462.858 kb on + strand at 4462.872 kb on - strand at 4462.872 kb on - strand at 4462.883 kb on - strand at 4462.883 kb on - strand at 4462.964 kb on - strand, within treB at 4462.964 kb on - strand, within treB at 4462.965 kb on + strand, within treB at 4462.976 kb on - strand, within treB at 4462.982 kb on + strand, within treB at 4462.982 kb on + strand, within treB at 4463.014 kb on - strand, within treB at 4463.045 kb on - strand, within treB at 4463.155 kb on + strand, within treB at 4463.184 kb on - strand, within treB at 4463.185 kb on + strand, within treB at 4463.193 kb on - strand, within treB at 4463.193 kb on - strand, within treB at 4463.194 kb on - strand, within treB at 4463.202 kb on - strand, within treB at 4463.228 kb on + strand, within treB at 4463.237 kb on + strand, within treB at 4463.237 kb on + strand, within treB at 4463.237 kb on + strand, within treB at 4463.241 kb on - strand, within treB at 4463.260 kb on - strand, within treB at 4463.261 kb on - strand, within treB at 4463.261 kb on - strand, within treB at 4463.297 kb on + strand, within treB at 4463.297 kb on + strand, within treB at 4463.300 kb on - strand, within treB at 4463.300 kb on - strand, within treB at 4463.305 kb on - strand, within treB at 4463.319 kb on - strand, within treB at 4463.319 kb on - strand, within treB at 4463.356 kb on + strand, within treB at 4463.360 kb on - strand, within treB at 4463.364 kb on - strand, within treB at 4463.373 kb on - strand, within treB at 4463.373 kb on - strand, within treB at 4463.382 kb on + strand, within treB at 4463.412 kb on + strand, within treB at 4463.426 kb on + strand, within treB at 4463.431 kb on - strand, within treB at 4463.509 kb on + strand, within treB at 4463.519 kb on + strand, within treB at 4463.561 kb on - strand at 4463.585 kb on + strand, within treB at 4463.585 kb on + strand, within treB at 4463.640 kb on - strand, within treB at 4463.640 kb on - strand, within treB
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 1 mM remove 4,460,100 + nrdD b4238 0.73 +0.2 4,460,168 - nrdD b4238 0.76 -0.1 4,460,186 + nrdD b4238 0.77 -0.4 4,460,196 + nrdD b4238 0.77 +0.5 4,460,196 + nrdD b4238 0.77 +0.0 4,460,218 + nrdD b4238 0.78 +0.5 4,460,225 + nrdD b4238 0.79 -0.0 4,460,225 + nrdD b4238 0.79 +0.0 4,460,238 - nrdD b4238 0.79 -0.6 4,460,243 + nrdD b4238 0.79 +0.6 4,460,260 - nrdD b4238 0.80 +0.0 4,460,360 - nrdD b4238 0.85 -0.3 4,460,371 + nrdD b4238 0.85 -0.1 4,460,371 + nrdD b4238 0.85 -0.2 4,460,387 + nrdD b4238 0.86 +0.1 4,460,447 - nrdD b4238 0.89 -0.2 4,460,447 - nrdD b4238 0.89 -0.0 4,460,504 - +0.2 4,460,504 - -0.5 4,460,542 - -1.4 4,460,547 - +0.1 4,460,554 + -0.0 4,460,661 + +0.3 4,460,820 + +0.0 4,460,876 + +0.3 4,460,891 - -0.4 4,460,891 - -0.7 4,461,038 + -0.7 4,461,038 + +0.4 4,461,080 + +0.2 4,461,097 - +1.2 4,461,128 + -1.7 4,461,145 + -0.7 4,461,190 - -1.9 4,461,295 + treC b4239 0.13 +0.5 4,461,314 + treC b4239 0.14 -1.9 4,461,427 + treC b4239 0.21 -1.0 4,461,533 + treC b4239 0.28 +0.1 4,461,533 + treC b4239 0.28 -0.9 4,461,857 - treC b4239 0.47 +0.1 4,461,957 - treC b4239 0.53 -0.0 4,461,977 - treC b4239 0.54 -1.8 4,462,007 + treC b4239 0.56 -1.5 4,462,145 + treC b4239 0.64 +0.0 4,462,223 - treC b4239 0.69 -0.1 4,462,251 + treC b4239 0.71 -0.0 4,462,328 + treC b4239 0.76 -1.5 4,462,328 + treC b4239 0.76 -1.5 4,462,333 + treC b4239 0.76 -1.6 4,462,358 - treC b4239 0.77 -0.9 4,462,415 + treC b4239 0.81 -1.3 4,462,421 + treC b4239 0.81 +0.2 4,462,421 + treC b4239 0.81 -1.8 4,462,422 - treC b4239 0.81 +0.1 4,462,447 - treC b4239 0.83 +0.1 4,462,447 - treC b4239 0.83 +0.5 4,462,526 + treC b4239 0.88 +0.5 4,462,526 + treC b4239 0.88 -0.5 4,462,613 + -0.2 4,462,655 + -0.0 4,462,736 - +0.4 4,462,742 - +0.2 4,462,742 - +0.3 4,462,747 - -0.3 4,462,810 - +0.3 4,462,810 - -0.8 4,462,824 + +0.3 4,462,840 - +0.3 4,462,858 + -0.5 4,462,872 - +0.3 4,462,872 - -1.3 4,462,883 - +0.2 4,462,883 - +0.0 4,462,964 - treB b4240 0.13 +0.5 4,462,964 - treB b4240 0.13 -1.5 4,462,965 + treB b4240 0.13 +0.6 4,462,976 - treB b4240 0.14 +0.3 4,462,982 + treB b4240 0.14 +0.9 4,462,982 + treB b4240 0.14 -0.1 4,463,014 - treB b4240 0.16 -0.3 4,463,045 - treB b4240 0.18 -0.4 4,463,155 + treB b4240 0.26 +1.4 4,463,184 - treB b4240 0.28 -0.5 4,463,185 + treB b4240 0.28 -0.6 4,463,193 - treB b4240 0.29 +0.1 4,463,193 - treB b4240 0.29 -0.6 4,463,194 - treB b4240 0.29 +0.2 4,463,202 - treB b4240 0.30 +0.6 4,463,228 + treB b4240 0.31 +0.4 4,463,237 + treB b4240 0.32 +0.4 4,463,237 + treB b4240 0.32 +0.7 4,463,237 + treB b4240 0.32 +0.4 4,463,241 - treB b4240 0.32 -0.4 4,463,260 - treB b4240 0.34 -1.0 4,463,261 - treB b4240 0.34 +0.1 4,463,261 - treB b4240 0.34 +1.3 4,463,297 + treB b4240 0.36 +0.4 4,463,297 + treB b4240 0.36 +0.2 4,463,300 - treB b4240 0.36 +1.0 4,463,300 - treB b4240 0.36 +0.7 4,463,305 - treB b4240 0.37 -0.9 4,463,319 - treB b4240 0.38 -0.7 4,463,319 - treB b4240 0.38 +1.4 4,463,356 + treB b4240 0.40 -0.2 4,463,360 - treB b4240 0.41 -0.2 4,463,364 - treB b4240 0.41 -0.8 4,463,373 - treB b4240 0.42 +1.3 4,463,373 - treB b4240 0.42 +0.2 4,463,382 + treB b4240 0.42 +0.2 4,463,412 + treB b4240 0.44 -0.5 4,463,426 + treB b4240 0.45 +0.3 4,463,431 - treB b4240 0.46 +0.3 4,463,509 + treB b4240 0.51 +0.6 4,463,519 + treB b4240 0.52 +0.3 4,463,561 - +0.1 4,463,585 + treB b4240 0.56 -0.4 4,463,585 + treB b4240 0.56 +0.0 4,463,640 - treB b4240 0.60 +0.9 4,463,640 - treB b4240 0.60 +0.3
Or see this region's nucleotide sequence