Experiment: 2,4-Dinitrophenol 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recD and recB overlap by 1 nucleotides recB and ptr overlap by 8 nucleotides
b2819: recD - exonuclease V (RecBCD complex), alpha chain (NCBI), at 2,948,657 to 2,950,483
recD
b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025
recB
b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906
ptr
Position (kb)
2950
2951
2952
2953
2954
2955 Strain fitness (log2 ratio)
-2
-1
0
1 at 2949.559 kb on - strand, within recD at 2949.564 kb on - strand, within recD at 2949.648 kb on + strand, within recD at 2949.648 kb on - strand, within recD at 2949.648 kb on - strand, within recD at 2949.658 kb on + strand, within recD at 2949.691 kb on - strand, within recD at 2949.691 kb on - strand, within recD at 2949.911 kb on - strand, within recD at 2950.492 kb on + strand at 2950.492 kb on + strand at 2950.500 kb on - strand at 2951.352 kb on - strand, within recB at 2951.941 kb on + strand, within recB at 2952.471 kb on - strand, within recB at 2952.820 kb on + strand, within recB at 2953.040 kb on - strand, within recB at 2953.978 kb on + strand at 2953.978 kb on + strand at 2954.006 kb on - strand at 2954.072 kb on + strand at 2954.072 kb on + strand at 2954.201 kb on - strand at 2954.359 kb on + strand, within ptr at 2954.508 kb on + strand, within ptr at 2954.516 kb on + strand, within ptr at 2954.587 kb on - strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.713 kb on - strand, within ptr at 2954.724 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.777 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 1 mM remove 2,949,559 - recD b2819 0.49 +0.7 2,949,564 - recD b2819 0.50 -0.2 2,949,648 + recD b2819 0.54 -0.3 2,949,648 - recD b2819 0.54 -0.2 2,949,648 - recD b2819 0.54 -0.2 2,949,658 + recD b2819 0.55 -0.6 2,949,691 - recD b2819 0.57 +1.2 2,949,691 - recD b2819 0.57 -1.8 2,949,911 - recD b2819 0.69 -0.5 2,950,492 + +0.1 2,950,492 + -1.1 2,950,500 - +1.0 2,951,352 - recB b2820 0.25 -2.3 2,951,941 + recB b2820 0.41 -1.5 2,952,471 - recB b2820 0.56 -0.9 2,952,820 + recB b2820 0.66 -2.1 2,953,040 - recB b2820 0.72 -0.4 2,953,978 + -0.5 2,953,978 + -0.2 2,954,006 - -1.1 2,954,072 + -0.0 2,954,072 + +0.1 2,954,201 - -0.2 2,954,359 + ptr b2821 0.12 +0.9 2,954,508 + ptr b2821 0.17 +0.4 2,954,516 + ptr b2821 0.17 +0.4 2,954,587 - ptr b2821 0.20 -1.3 2,954,617 + ptr b2821 0.21 -0.1 2,954,617 + ptr b2821 0.21 +0.8 2,954,626 - ptr b2821 0.21 -0.3 2,954,626 - ptr b2821 0.21 -1.3 2,954,713 - ptr b2821 0.24 -0.0 2,954,724 - ptr b2821 0.24 -0.1 2,954,759 - ptr b2821 0.26 -0.0 2,954,759 - ptr b2821 0.26 -1.1 2,954,771 - ptr b2821 0.26 +0.0 2,954,771 - ptr b2821 0.26 +0.1 2,954,777 - ptr b2821 0.26 +0.5 2,954,782 - ptr b2821 0.26 +0.4 2,954,782 - ptr b2821 0.26 -1.7 2,954,857 + ptr b2821 0.29 +0.9 2,954,857 + ptr b2821 0.29 +0.7 2,954,905 + ptr b2821 0.31 +0.3 2,954,905 + ptr b2821 0.31 +0.8 2,954,985 + ptr b2821 0.33 -0.1 2,954,985 + ptr b2821 0.33 +0.8
Or see this region's nucleotide sequence