Experiment: 2,4-Dinitrophenol 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoG and nuoF are separated by 52 nucleotides nuoF and nuoE overlap by 4 nucleotides nuoE and nuoC are separated by 2 nucleotides
b2283: nuoG - NADH:ubiquinone oxidoreductase, chain G (RefSeq), at 2,395,461 to 2,398,187
nuoG
b2284: nuoF - NADH:ubiquinone oxidoreductase, chain F (NCBI), at 2,398,240 to 2,399,577
nuoF
b2285: nuoE - NADH dehydrogenase subunit E (NCBI), at 2,399,574 to 2,400,074
nuoE
b2286: nuoC - NADH:ubiquinone oxidoreductase, chain C,D (RefSeq), at 2,400,077 to 2,401,867
nuoC
Position (kb)
2398
2399
2400 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2397.247 kb on - strand, within nuoG at 2397.247 kb on - strand, within nuoG at 2397.264 kb on - strand, within nuoG at 2397.522 kb on + strand, within nuoG at 2397.522 kb on + strand, within nuoG at 2397.772 kb on - strand, within nuoG at 2397.785 kb on + strand, within nuoG at 2398.076 kb on - strand at 2398.076 kb on - strand at 2398.222 kb on - strand at 2398.338 kb on + strand at 2398.349 kb on + strand at 2398.351 kb on + strand at 2398.351 kb on + strand at 2398.359 kb on - strand at 2398.367 kb on + strand at 2398.449 kb on + strand, within nuoF at 2398.487 kb on + strand, within nuoF at 2398.570 kb on + strand, within nuoF at 2398.573 kb on - strand, within nuoF at 2398.610 kb on + strand, within nuoF at 2398.770 kb on + strand, within nuoF at 2398.778 kb on + strand, within nuoF at 2398.795 kb on - strand, within nuoF at 2398.831 kb on - strand, within nuoF at 2398.874 kb on - strand, within nuoF at 2398.876 kb on + strand, within nuoF at 2398.883 kb on + strand, within nuoF at 2398.914 kb on + strand, within nuoF at 2398.914 kb on + strand at 2398.964 kb on + strand, within nuoF at 2398.964 kb on + strand, within nuoF at 2399.113 kb on - strand, within nuoF at 2399.283 kb on - strand, within nuoF at 2399.293 kb on - strand, within nuoF at 2399.314 kb on - strand, within nuoF at 2399.405 kb on - strand, within nuoF at 2399.405 kb on - strand, within nuoF at 2399.406 kb on - strand, within nuoF at 2399.461 kb on + strand at 2399.461 kb on + strand at 2399.508 kb on - strand at 2399.626 kb on + strand at 2399.626 kb on + strand, within nuoE at 2399.725 kb on + strand, within nuoE at 2399.863 kb on - strand, within nuoE at 2399.948 kb on - strand, within nuoE at 2399.972 kb on + strand, within nuoE at 2400.031 kb on - strand at 2400.113 kb on + strand at 2400.113 kb on + strand at 2400.221 kb on - strand at 2400.221 kb on - strand at 2400.254 kb on + strand at 2400.254 kb on - strand at 2400.327 kb on - strand, within nuoC at 2400.349 kb on - strand, within nuoC at 2400.357 kb on - strand, within nuoC at 2400.431 kb on + strand, within nuoC at 2400.565 kb on - strand, within nuoC
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 1 mM remove 2,397,247 - nuoG b2283 0.65 -1.5 2,397,247 - nuoG b2283 0.65 -2.0 2,397,264 - nuoG b2283 0.66 -0.8 2,397,522 + nuoG b2283 0.76 -1.4 2,397,522 + nuoG b2283 0.76 -1.8 2,397,772 - nuoG b2283 0.85 -1.8 2,397,785 + nuoG b2283 0.85 -1.1 2,398,076 - +0.6 2,398,076 - -0.4 2,398,222 - -1.6 2,398,338 + -1.0 2,398,349 + -1.8 2,398,351 + +1.0 2,398,351 + -2.0 2,398,359 - -0.3 2,398,367 + -1.1 2,398,449 + nuoF b2284 0.16 -2.1 2,398,487 + nuoF b2284 0.18 +0.1 2,398,570 + nuoF b2284 0.25 -2.4 2,398,573 - nuoF b2284 0.25 -3.0 2,398,610 + nuoF b2284 0.28 -1.4 2,398,770 + nuoF b2284 0.40 -1.1 2,398,778 + nuoF b2284 0.40 -1.2 2,398,795 - nuoF b2284 0.41 -2.9 2,398,831 - nuoF b2284 0.44 -0.9 2,398,874 - nuoF b2284 0.47 -2.1 2,398,876 + nuoF b2284 0.48 +0.0 2,398,883 + nuoF b2284 0.48 -2.7 2,398,914 + nuoF b2284 0.50 +0.8 2,398,914 + -0.4 2,398,964 + nuoF b2284 0.54 -0.5 2,398,964 + nuoF b2284 0.54 -1.0 2,399,113 - nuoF b2284 0.65 -1.5 2,399,283 - nuoF b2284 0.78 -0.2 2,399,293 - nuoF b2284 0.79 -1.3 2,399,314 - nuoF b2284 0.80 +0.3 2,399,405 - nuoF b2284 0.87 -0.9 2,399,405 - nuoF b2284 0.87 -0.9 2,399,406 - nuoF b2284 0.87 -0.3 2,399,461 + -0.4 2,399,461 + -0.1 2,399,508 - -0.0 2,399,626 + +0.3 2,399,626 + nuoE b2285 0.10 -1.1 2,399,725 + nuoE b2285 0.30 -2.2 2,399,863 - nuoE b2285 0.58 +0.5 2,399,948 - nuoE b2285 0.75 -1.1 2,399,972 + nuoE b2285 0.79 -3.1 2,400,031 - -0.3 2,400,113 + -0.3 2,400,113 + -1.6 2,400,221 - -2.0 2,400,221 - -1.5 2,400,254 + -2.0 2,400,254 - -0.8 2,400,327 - nuoC b2286 0.14 -0.4 2,400,349 - nuoC b2286 0.15 -1.0 2,400,357 - nuoC b2286 0.16 -0.8 2,400,431 + nuoC b2286 0.20 -0.5 2,400,565 - nuoC b2286 0.27 -2.4
Or see this region's nucleotide sequence