Experiment: 2,4-Dinitrophenol 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gatC and gatB are separated by 3 nucleotides gatB and gatA are separated by 30 nucleotides gatA and gatZ are separated by 9 nucleotides gatZ and gatY are separated by 28 nucleotides
b2092: gatC - galactitol-specific enzyme IIC component of PTS (NCBI), at 2,170,945 to 2,172,300
gatC
b2093: gatB - galactitol-specific enzyme IIB component of PTS (NCBI), at 2,172,304 to 2,172,588
gatB
b2094: gatA - galactitol-specific enzyme IIA component of PTS (NCBI), at 2,172,619 to 2,173,071
gatA
b2095: gatZ - D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI), at 2,173,081 to 2,174,343
gatZ
b2096: gatY - tagatose-bisphosphate aldolase 1 (VIMSS), at 2,174,372 to 2,175,226
gatY
Position (kb)
2173
2174
2175 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2172.130 kb on + strand, within gatC at 2172.137 kb on - strand, within gatC at 2172.247 kb on + strand at 2172.255 kb on + strand at 2172.285 kb on - strand at 2172.285 kb on - strand at 2172.295 kb on + strand at 2172.296 kb on - strand at 2172.303 kb on - strand at 2172.305 kb on - strand at 2172.315 kb on - strand at 2172.430 kb on - strand, within gatB at 2172.542 kb on - strand, within gatB at 2172.552 kb on - strand, within gatB at 2172.688 kb on - strand, within gatA at 2172.690 kb on - strand, within gatA at 2172.690 kb on - strand, within gatA at 2172.716 kb on - strand, within gatA at 2172.726 kb on - strand, within gatA at 2172.807 kb on - strand, within gatA at 2172.839 kb on - strand, within gatA at 2172.917 kb on - strand, within gatA at 2172.978 kb on - strand, within gatA at 2172.978 kb on - strand, within gatA at 2172.983 kb on + strand, within gatA at 2173.004 kb on - strand, within gatA at 2173.067 kb on + strand at 2173.075 kb on - strand at 2173.177 kb on - strand at 2173.177 kb on - strand at 2173.197 kb on - strand at 2173.197 kb on - strand at 2173.205 kb on - strand at 2173.205 kb on - strand at 2173.233 kb on - strand, within gatZ at 2173.440 kb on - strand, within gatZ at 2173.444 kb on - strand, within gatZ at 2173.451 kb on + strand, within gatZ at 2173.451 kb on + strand, within gatZ at 2173.459 kb on - strand, within gatZ at 2173.459 kb on - strand, within gatZ at 2173.485 kb on - strand, within gatZ at 2173.588 kb on - strand, within gatZ at 2173.638 kb on - strand, within gatZ at 2173.717 kb on - strand, within gatZ at 2173.783 kb on + strand, within gatZ at 2173.791 kb on - strand, within gatZ at 2173.791 kb on - strand, within gatZ at 2173.870 kb on - strand, within gatZ at 2174.277 kb on + strand at 2174.277 kb on + strand at 2174.285 kb on - strand at 2174.285 kb on - strand at 2174.285 kb on - strand at 2174.371 kb on + strand at 2174.422 kb on - strand at 2174.422 kb on - strand at 2174.469 kb on + strand, within gatY at 2174.749 kb on + strand, within gatY at 2174.749 kb on + strand, within gatY at 2175.002 kb on + strand, within gatY at 2175.029 kb on + strand, within gatY at 2175.129 kb on + strand, within gatY at 2175.189 kb on + strand at 2175.226 kb on + strand at 2175.226 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 1 mM remove 2,172,130 + gatC b2092 0.87 +2.4 2,172,137 - gatC b2092 0.88 +3.7 2,172,247 + +4.5 2,172,255 + +4.4 2,172,285 - +4.5 2,172,285 - +4.4 2,172,295 + +4.8 2,172,296 - +4.4 2,172,303 - +5.3 2,172,305 - +4.5 2,172,315 - +3.7 2,172,430 - gatB b2093 0.44 +2.9 2,172,542 - gatB b2093 0.84 +1.3 2,172,552 - gatB b2093 0.87 +2.3 2,172,688 - gatA b2094 0.15 +0.4 2,172,690 - gatA b2094 0.16 +1.3 2,172,690 - gatA b2094 0.16 +1.0 2,172,716 - gatA b2094 0.21 +0.1 2,172,726 - gatA b2094 0.24 -1.2 2,172,807 - gatA b2094 0.42 +0.5 2,172,839 - gatA b2094 0.49 +1.0 2,172,917 - gatA b2094 0.66 +1.7 2,172,978 - gatA b2094 0.79 +2.2 2,172,978 - gatA b2094 0.79 +2.0 2,172,983 + gatA b2094 0.80 +4.8 2,173,004 - gatA b2094 0.85 +2.5 2,173,067 + +4.5 2,173,075 - +1.1 2,173,177 - -0.1 2,173,177 - +0.5 2,173,197 - +1.5 2,173,197 - +0.6 2,173,205 - +2.3 2,173,205 - +1.3 2,173,233 - gatZ b2095 0.12 +1.7 2,173,440 - gatZ b2095 0.28 +2.5 2,173,444 - gatZ b2095 0.29 +1.9 2,173,451 + gatZ b2095 0.29 +4.7 2,173,451 + gatZ b2095 0.29 +4.9 2,173,459 - gatZ b2095 0.30 +1.6 2,173,459 - gatZ b2095 0.30 +1.1 2,173,485 - gatZ b2095 0.32 +2.1 2,173,588 - gatZ b2095 0.40 +1.4 2,173,638 - gatZ b2095 0.44 +3.4 2,173,717 - gatZ b2095 0.50 +2.2 2,173,783 + gatZ b2095 0.56 +4.7 2,173,791 - gatZ b2095 0.56 +3.6 2,173,791 - gatZ b2095 0.56 +2.8 2,173,870 - gatZ b2095 0.62 +1.6 2,174,277 + +5.0 2,174,277 + +5.0 2,174,285 - +4.5 2,174,285 - +4.0 2,174,285 - +4.6 2,174,371 + +4.7 2,174,422 - -0.2 2,174,422 - +0.6 2,174,469 + gatY b2096 0.11 +4.2 2,174,749 + gatY b2096 0.44 +4.5 2,174,749 + gatY b2096 0.44 +4.2 2,175,002 + gatY b2096 0.74 +4.8 2,175,029 + gatY b2096 0.77 +4.7 2,175,129 + gatY b2096 0.89 +4.9 2,175,189 + +4.5 2,175,226 + +4.6 2,175,226 + +4.5
Or see this region's nucleotide sequence