Experiment: 2,4-Dinitrophenol 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potD and potC overlap by 4 nucleotides potC and potB overlap by 4 nucleotides potB and potA are separated by 13 nucleotides
b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052
potD
b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843
potC
b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667
potB
b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817
potA
Position (kb)
1182
1183 Strain fitness (log2 ratio)
-2
-1
0
1 at 1181.101 kb on + strand at 1181.101 kb on + strand at 1181.170 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.421 kb on + strand, within potD at 1181.480 kb on - strand, within potD at 1181.522 kb on - strand, within potD at 1181.539 kb on + strand, within potD at 1181.547 kb on - strand, within potD at 1181.547 kb on - strand, within potD at 1181.556 kb on + strand, within potD at 1181.556 kb on + strand, within potD at 1181.581 kb on - strand, within potD at 1181.684 kb on - strand, within potD at 1181.721 kb on - strand, within potD at 1181.839 kb on - strand, within potD at 1181.865 kb on - strand, within potD at 1181.918 kb on + strand, within potD at 1182.003 kb on + strand at 1182.395 kb on + strand, within potC at 1182.474 kb on - strand, within potC at 1182.476 kb on - strand, within potC at 1182.524 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.589 kb on + strand, within potC at 1182.668 kb on + strand, within potC at 1182.691 kb on + strand, within potC at 1182.700 kb on + strand, within potC at 1182.730 kb on + strand, within potC at 1182.910 kb on + strand at 1182.910 kb on + strand at 1182.914 kb on - strand at 1183.032 kb on + strand, within potB at 1183.032 kb on + strand, within potB at 1183.193 kb on - strand, within potB at 1183.202 kb on + strand, within potB at 1183.202 kb on + strand, within potB at 1183.283 kb on + strand, within potB at 1183.449 kb on + strand, within potB at 1183.542 kb on - strand, within potB at 1183.680 kb on - strand at 1183.698 kb on - strand at 1183.698 kb on - strand at 1183.711 kb on - strand at 1183.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 1 mM remove 1,181,101 + -0.8 1,181,101 + -1.1 1,181,170 - potD b1123 0.16 +0.4 1,181,280 - potD b1123 0.26 -1.1 1,181,280 - potD b1123 0.26 -0.3 1,181,421 + potD b1123 0.40 -1.0 1,181,480 - potD b1123 0.45 +1.2 1,181,522 - potD b1123 0.49 +0.5 1,181,539 + potD b1123 0.51 -0.5 1,181,547 - potD b1123 0.52 -2.7 1,181,547 - potD b1123 0.52 -0.3 1,181,556 + potD b1123 0.53 -0.3 1,181,556 + potD b1123 0.53 -0.3 1,181,581 - potD b1123 0.55 +0.5 1,181,684 - potD b1123 0.65 -1.4 1,181,721 - potD b1123 0.68 -1.7 1,181,839 - potD b1123 0.80 +0.7 1,181,865 - potD b1123 0.82 -0.5 1,181,918 + potD b1123 0.87 -0.1 1,182,003 + -0.5 1,182,395 + potC b1124 0.44 -1.3 1,182,474 - potC b1124 0.53 -0.9 1,182,476 - potC b1124 0.54 -0.7 1,182,524 + potC b1124 0.60 -1.3 1,182,581 + potC b1124 0.67 -2.4 1,182,581 + potC b1124 0.67 -2.0 1,182,589 + potC b1124 0.68 -0.9 1,182,668 + potC b1124 0.78 -0.3 1,182,691 + potC b1124 0.81 -0.5 1,182,700 + potC b1124 0.82 -0.6 1,182,730 + potC b1124 0.86 -0.6 1,182,910 + +0.1 1,182,910 + -0.5 1,182,914 - -0.3 1,183,032 + potB b1125 0.23 -1.0 1,183,032 + potB b1125 0.23 +0.3 1,183,193 - potB b1125 0.43 -1.6 1,183,202 + potB b1125 0.44 +0.4 1,183,202 + potB b1125 0.44 -1.4 1,183,283 + potB b1125 0.54 -2.7 1,183,449 + potB b1125 0.74 -0.2 1,183,542 - potB b1125 0.85 -0.8 1,183,680 - -0.0 1,183,698 - -0.5 1,183,698 - +0.9 1,183,711 - +0.1 1,183,711 - -0.4
Or see this region's nucleotide sequence