Experiment: 2,4-Dinitrophenol 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeO and yaeP overlap by 8 nucleotides yaeP and yaeQ are separated by 165 nucleotides yaeQ and yaeJ overlap by 4 nucleotides yaeJ and nlpE are separated by 13 nucleotides nlpE and yaeF are separated by 199 nucleotides
b0189: yaeO - orf, hypothetical protein (VIMSS), at 213,678 to 213,932
yaeO
b4406: yaeP - hypothetical protein (NCBI), at 213,925 to 214,125
yaeP
b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836
yaeQ
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
b0192: nlpE - lipoprotein involved with copper homeostasis and adhesion (NCBI), at 215,269 to 215,979
nlpE
b0193: yaeF - orf, hypothetical protein (VIMSS), at 216,179 to 217,003
yaeF
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 213.880 kb on - strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.913 kb on + strand at 213.913 kb on + strand at 213.956 kb on - strand, within yaeP at 213.956 kb on - strand, within yaeP at 214.040 kb on - strand, within yaeP at 214.130 kb on + strand at 214.142 kb on + strand at 214.166 kb on + strand at 214.174 kb on - strand at 214.174 kb on - strand at 214.216 kb on - strand at 214.369 kb on - strand, within yaeQ at 214.391 kb on - strand, within yaeQ at 214.404 kb on - strand, within yaeQ at 214.419 kb on - strand, within yaeQ at 214.468 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.542 kb on + strand, within yaeQ at 214.572 kb on + strand, within yaeQ at 214.574 kb on + strand, within yaeQ at 214.600 kb on - strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.764 kb on - strand, within yaeQ at 214.773 kb on - strand, within yaeQ at 214.808 kb on + strand at 214.825 kb on - strand at 214.836 kb on - strand at 214.872 kb on + strand at 214.874 kb on + strand at 214.919 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.091 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.299 kb on + strand at 215.372 kb on + strand, within nlpE at 215.378 kb on + strand, within nlpE at 215.410 kb on + strand, within nlpE at 215.476 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.558 kb on + strand, within nlpE at 215.582 kb on - strand, within nlpE at 215.621 kb on + strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.694 kb on + strand, within nlpE at 215.756 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.824 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.897 kb on - strand, within nlpE at 215.915 kb on - strand at 215.915 kb on - strand at 215.931 kb on - strand at 215.931 kb on - strand at 215.933 kb on - strand at 215.933 kb on - strand at 215.958 kb on + strand at 215.968 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.151 kb on + strand at 216.159 kb on - strand at 216.159 kb on - strand at 216.160 kb on - strand at 216.160 kb on - strand at 216.235 kb on + strand at 216.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 1 mM remove 213,880 - yaeO b0189 0.79 -0.2 213,880 - yaeO b0189 0.79 +0.1 213,913 + +0.7 213,913 + +0.0 213,956 - yaeP b4406 0.15 +0.2 213,956 - yaeP b4406 0.15 +0.3 214,040 - yaeP b4406 0.57 -0.2 214,130 + -0.8 214,142 + -1.7 214,166 + -0.8 214,174 - +0.4 214,174 - +0.4 214,216 - +0.5 214,369 - yaeQ b0190 0.14 -0.6 214,391 - yaeQ b0190 0.18 +0.8 214,404 - yaeQ b0190 0.21 +0.5 214,419 - yaeQ b0190 0.23 +0.3 214,468 + yaeQ b0190 0.32 -0.3 214,487 + yaeQ b0190 0.36 -0.3 214,487 + yaeQ b0190 0.36 +0.5 214,542 + yaeQ b0190 0.46 -0.2 214,572 + yaeQ b0190 0.51 -0.7 214,574 + yaeQ b0190 0.52 -1.6 214,600 - yaeQ b0190 0.57 -0.6 214,606 + yaeQ b0190 0.58 -1.1 214,606 + yaeQ b0190 0.58 +0.2 214,764 - yaeQ b0190 0.87 -0.1 214,773 - yaeQ b0190 0.88 +0.2 214,808 + +0.1 214,825 - -0.0 214,836 - -0.3 214,872 + -0.2 214,874 + -0.7 214,919 + yaeJ b0191 0.20 -0.7 215,045 + yaeJ b0191 0.50 -0.9 215,045 + yaeJ b0191 0.50 -1.4 215,091 + yaeJ b0191 0.61 -0.7 215,123 + yaeJ b0191 0.69 -1.7 215,123 + yaeJ b0191 0.69 -0.6 215,299 + +0.0 215,372 + nlpE b0192 0.14 -0.1 215,378 + nlpE b0192 0.15 +0.1 215,410 + nlpE b0192 0.20 +0.9 215,476 - nlpE b0192 0.29 -0.0 215,498 - nlpE b0192 0.32 +0.4 215,498 - nlpE b0192 0.32 +0.7 215,558 + nlpE b0192 0.41 +0.7 215,582 - nlpE b0192 0.44 +0.2 215,621 + nlpE b0192 0.50 -1.2 215,635 - nlpE b0192 0.51 +1.3 215,635 - nlpE b0192 0.51 -0.1 215,694 + nlpE b0192 0.60 -0.1 215,756 + nlpE b0192 0.68 +0.0 215,816 + nlpE b0192 0.77 -0.7 215,816 + nlpE b0192 0.77 +1.1 215,824 - nlpE b0192 0.78 -0.2 215,864 - nlpE b0192 0.84 -0.1 215,864 - nlpE b0192 0.84 +1.5 215,895 - nlpE b0192 0.88 +0.5 215,895 - nlpE b0192 0.88 -2.1 215,897 - nlpE b0192 0.88 +0.2 215,915 - -0.0 215,915 - -0.1 215,931 - +0.2 215,931 - +0.2 215,933 - +0.4 215,933 - -0.3 215,958 + -0.6 215,968 - -0.2 216,101 - -0.1 216,101 - -0.2 216,101 - +0.1 216,151 + +0.1 216,159 - +0.1 216,159 - +0.0 216,160 - +0.8 216,160 - +0.0 216,235 + +0.3 216,235 + +0.5
Or see this region's nucleotide sequence