Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF851

Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF849 and GFF850 are separated by 13 nucleotidesGFF850 and GFF851 are separated by 9 nucleotidesGFF851 and GFF852 overlap by 1 nucleotidesGFF852 and GFF853 are separated by 128 nucleotides GFF849 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 30,310 to 30,774 GFF849 GFF850 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (EC 4.1.1.85) (L-ascorbate utilization protein D), at 30,788 to 31,438 GFF850 GFF851 - L-ribulose-5-phosphate 3-epimerase UlaE (EC 5.1.3.22) (L-ascorbate utilization protein E), at 31,448 to 32,302 GFF851 GFF852 - L-ribulose-5-phosphate 4-epimerase UlaF (EC 5.1.3.4) (L-ascorbate utilization protein F), at 32,302 to 32,988 GFF852 GFF853 - UPF0379 protein yjfY precursor, at 33,117 to 33,392 GFF853 Position (kb) 31 32 33Strain fitness (log2 ratio) -1 0 1at 30.772 kb on + strandat 31.009 kb on + strand, within GFF850at 31.010 kb on - strand, within GFF850at 31.191 kb on - strand, within GFF850at 31.741 kb on + strand, within GFF851at 31.748 kb on - strand, within GFF851at 31.811 kb on + strand, within GFF851at 31.811 kb on + strand, within GFF851at 31.815 kb on - strand, within GFF851at 31.861 kb on - strand, within GFF851at 31.946 kb on - strand, within GFF851at 31.967 kb on + strand, within GFF851at 31.968 kb on - strand, within GFF851at 31.976 kb on - strand, within GFF851at 32.034 kb on + strand, within GFF851at 32.089 kb on + strand, within GFF851at 32.093 kb on - strand, within GFF851at 32.189 kb on + strand, within GFF851at 32.325 kb on - strandat 32.325 kb on - strandat 32.370 kb on + strandat 32.422 kb on - strand, within GFF852at 32.457 kb on - strand, within GFF852at 32.479 kb on - strand, within GFF852at 32.545 kb on + strand, within GFF852at 32.716 kb on + strand, within GFF852at 32.785 kb on - strand, within GFF852at 32.841 kb on - strand, within GFF852at 32.845 kb on - strand, within GFF852at 32.926 kb on + strandat 32.926 kb on + strandat 32.953 kb on - strandat 33.012 kb on - strandat 33.125 kb on - strandat 33.128 kb on + strandat 33.178 kb on - strand, within GFF853at 33.178 kb on - strand, within GFF853at 33.188 kb on - strand, within GFF853at 33.188 kb on - strand, within GFF853at 33.190 kb on + strand, within GFF853at 33.190 kb on + strand, within GFF853at 33.191 kb on - strand, within GFF853at 33.258 kb on - strand, within GFF853

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
remove
30,772 + -0.3
31,009 + GFF850 0.34 -0.1
31,010 - GFF850 0.34 -0.2
31,191 - GFF850 0.62 +0.4
31,741 + GFF851 0.34 -0.2
31,748 - GFF851 0.35 -0.2
31,811 + GFF851 0.42 +0.2
31,811 + GFF851 0.42 -0.5
31,815 - GFF851 0.43 -0.9
31,861 - GFF851 0.48 +0.5
31,946 - GFF851 0.58 +0.4
31,967 + GFF851 0.61 -0.3
31,968 - GFF851 0.61 -0.3
31,976 - GFF851 0.62 -0.1
32,034 + GFF851 0.69 -0.2
32,089 + GFF851 0.75 -0.3
32,093 - GFF851 0.75 +0.3
32,189 + GFF851 0.87 -0.9
32,325 - -1.2
32,325 - -0.0
32,370 + -0.1
32,422 - GFF852 0.17 -0.3
32,457 - GFF852 0.23 +0.1
32,479 - GFF852 0.26 +0.1
32,545 + GFF852 0.35 +0.2
32,716 + GFF852 0.60 -0.0
32,785 - GFF852 0.70 +0.1
32,841 - GFF852 0.78 +0.1
32,845 - GFF852 0.79 +0.5
32,926 + -0.2
32,926 + -0.1
32,953 - -0.6
33,012 - -0.2
33,125 - -0.1
33,128 + +0.2
33,178 - GFF853 0.22 +0.0
33,178 - GFF853 0.22 -0.4
33,188 - GFF853 0.26 -0.2
33,188 - GFF853 0.26 -0.5
33,190 + GFF853 0.26 +0.0
33,190 + GFF853 0.26 -0.6
33,191 - GFF853 0.27 -0.5
33,258 - GFF853 0.51 +0.1

Or see this region's nucleotide sequence