Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF849 and GFF850 are separated by 13 nucleotides GFF850 and GFF851 are separated by 9 nucleotides GFF851 and GFF852 overlap by 1 nucleotides GFF852 and GFF853 are separated by 128 nucleotides
GFF849 - Ascorbate-specific PTS system, EIIA component (EC 2.7.1.-), at 30,310 to 30,774
GFF849
GFF850 - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (EC 4.1.1.85) (L-ascorbate utilization protein D), at 30,788 to 31,438
GFF850
GFF851 - L-ribulose-5-phosphate 3-epimerase UlaE (EC 5.1.3.22) (L-ascorbate utilization protein E), at 31,448 to 32,302
GFF851
GFF852 - L-ribulose-5-phosphate 4-epimerase UlaF (EC 5.1.3.4) (L-ascorbate utilization protein F), at 32,302 to 32,988
GFF852
GFF853 - UPF0379 protein yjfY precursor, at 33,117 to 33,392
GFF853
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-1
0
1 at 30.772 kb on + strand at 31.009 kb on + strand, within GFF850 at 31.010 kb on - strand, within GFF850 at 31.191 kb on - strand, within GFF850 at 31.741 kb on + strand, within GFF851 at 31.748 kb on - strand, within GFF851 at 31.811 kb on + strand, within GFF851 at 31.811 kb on + strand, within GFF851 at 31.815 kb on - strand, within GFF851 at 31.861 kb on - strand, within GFF851 at 31.946 kb on - strand, within GFF851 at 31.967 kb on + strand, within GFF851 at 31.968 kb on - strand, within GFF851 at 31.976 kb on - strand, within GFF851 at 32.034 kb on + strand, within GFF851 at 32.089 kb on + strand, within GFF851 at 32.093 kb on - strand, within GFF851 at 32.189 kb on + strand, within GFF851 at 32.325 kb on - strand at 32.325 kb on - strand at 32.370 kb on + strand at 32.422 kb on - strand, within GFF852 at 32.457 kb on - strand, within GFF852 at 32.479 kb on - strand, within GFF852 at 32.545 kb on + strand, within GFF852 at 32.716 kb on + strand, within GFF852 at 32.785 kb on - strand, within GFF852 at 32.841 kb on - strand, within GFF852 at 32.845 kb on - strand, within GFF852 at 32.926 kb on + strand at 32.926 kb on + strand at 32.953 kb on - strand at 33.012 kb on - strand at 33.125 kb on - strand at 33.128 kb on + strand at 33.178 kb on - strand, within GFF853 at 33.178 kb on - strand, within GFF853 at 33.188 kb on - strand, within GFF853 at 33.188 kb on - strand, within GFF853 at 33.190 kb on + strand, within GFF853 at 33.190 kb on + strand, within GFF853 at 33.191 kb on - strand, within GFF853 at 33.258 kb on - strand, within GFF853
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 30,772 + -0.3 31,009 + GFF850 0.34 -0.1 31,010 - GFF850 0.34 -0.2 31,191 - GFF850 0.62 +0.4 31,741 + GFF851 0.34 -0.2 31,748 - GFF851 0.35 -0.2 31,811 + GFF851 0.42 +0.2 31,811 + GFF851 0.42 -0.5 31,815 - GFF851 0.43 -0.9 31,861 - GFF851 0.48 +0.5 31,946 - GFF851 0.58 +0.4 31,967 + GFF851 0.61 -0.3 31,968 - GFF851 0.61 -0.3 31,976 - GFF851 0.62 -0.1 32,034 + GFF851 0.69 -0.2 32,089 + GFF851 0.75 -0.3 32,093 - GFF851 0.75 +0.3 32,189 + GFF851 0.87 -0.9 32,325 - -1.2 32,325 - -0.0 32,370 + -0.1 32,422 - GFF852 0.17 -0.3 32,457 - GFF852 0.23 +0.1 32,479 - GFF852 0.26 +0.1 32,545 + GFF852 0.35 +0.2 32,716 + GFF852 0.60 -0.0 32,785 - GFF852 0.70 +0.1 32,841 - GFF852 0.78 +0.1 32,845 - GFF852 0.79 +0.5 32,926 + -0.2 32,926 + -0.1 32,953 - -0.6 33,012 - -0.2 33,125 - -0.1 33,128 + +0.2 33,178 - GFF853 0.22 +0.0 33,178 - GFF853 0.22 -0.4 33,188 - GFF853 0.26 -0.2 33,188 - GFF853 0.26 -0.5 33,190 + GFF853 0.26 +0.0 33,190 + GFF853 0.26 -0.6 33,191 - GFF853 0.27 -0.5 33,258 - GFF853 0.51 +0.1
Or see this region's nucleotide sequence