Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF582 and GFF583 overlap by 4 nucleotides GFF583 and GFF584 are separated by 337 nucleotides
GFF582 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 316,092 to 317,807
GFF582
GFF583 - Nitrate/nitrite transporter, at 317,804 to 319,138
GFF583
GFF584 - Glycerol dehydrogenase (EC 1.1.1.6), at 319,476 to 320,585
GFF584
Position (kb) 317
318
319
320 Strain fitness (log2 ratio) -1
0
1
2 at 316.894 kb on + strand, within GFF582 at 316.894 kb on + strand, within GFF582 at 317.030 kb on + strand, within GFF582 at 317.375 kb on + strand, within GFF582 at 317.375 kb on + strand, within GFF582 at 317.390 kb on + strand, within GFF582 at 317.391 kb on - strand, within GFF582 at 317.391 kb on - strand, within GFF582 at 317.479 kb on + strand, within GFF582 at 317.542 kb on + strand, within GFF582 at 317.543 kb on - strand, within GFF582 at 317.543 kb on - strand, within GFF582 at 317.689 kb on + strand at 317.897 kb on + strand at 317.898 kb on - strand at 317.898 kb on - strand at 318.074 kb on + strand, within GFF583 at 318.131 kb on - strand, within GFF583 at 318.170 kb on + strand, within GFF583 at 318.171 kb on - strand, within GFF583 at 318.244 kb on + strand, within GFF583 at 318.264 kb on + strand, within GFF583 at 318.264 kb on + strand, within GFF583 at 318.264 kb on + strand, within GFF583 at 318.265 kb on - strand, within GFF583 at 318.378 kb on + strand, within GFF583 at 318.379 kb on - strand, within GFF583 at 318.403 kb on - strand, within GFF583 at 318.403 kb on - strand, within GFF583 at 318.614 kb on - strand, within GFF583 at 318.634 kb on - strand, within GFF583 at 318.643 kb on + strand, within GFF583 at 318.673 kb on + strand, within GFF583 at 318.674 kb on - strand, within GFF583 at 318.768 kb on + strand, within GFF583 at 318.940 kb on + strand, within GFF583 at 318.941 kb on - strand, within GFF583 at 318.941 kb on - strand, within GFF583 at 318.942 kb on + strand, within GFF583 at 318.950 kb on - strand, within GFF583 at 319.049 kb on - strand at 319.049 kb on - strand at 319.050 kb on + strand at 319.050 kb on + strand at 319.087 kb on + strand at 319.302 kb on + strand at 319.481 kb on + strand at 319.820 kb on + strand, within GFF584 at 319.862 kb on + strand, within GFF584 at 319.867 kb on - strand, within GFF584 at 319.893 kb on + strand, within GFF584 at 319.911 kb on - strand, within GFF584 at 319.940 kb on + strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 320.094 kb on + strand, within GFF584 at 320.105 kb on - strand, within GFF584 at 320.105 kb on - strand, within GFF584 at 320.112 kb on + strand, within GFF584
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 316,894 + GFF582 0.47 -0.1 316,894 + GFF582 0.47 +0.4 317,030 + GFF582 0.55 -0.7 317,375 + GFF582 0.75 +0.1 317,375 + GFF582 0.75 +0.2 317,390 + GFF582 0.76 +0.2 317,391 - GFF582 0.76 +0.6 317,391 - GFF582 0.76 -0.6 317,479 + GFF582 0.81 -0.0 317,542 + GFF582 0.84 +0.4 317,543 - GFF582 0.85 +0.1 317,543 - GFF582 0.85 +0.3 317,689 + -0.1 317,897 + +0.3 317,898 - +2.5 317,898 - -0.1 318,074 + GFF583 0.20 -1.1 318,131 - GFF583 0.24 -0.1 318,170 + GFF583 0.27 +0.3 318,171 - GFF583 0.27 +0.0 318,244 + GFF583 0.33 +0.5 318,264 + GFF583 0.34 +0.6 318,264 + GFF583 0.34 +0.3 318,264 + GFF583 0.34 -1.4 318,265 - GFF583 0.35 +0.6 318,378 + GFF583 0.43 +0.6 318,379 - GFF583 0.43 +0.4 318,403 - GFF583 0.45 -0.0 318,403 - GFF583 0.45 -0.0 318,614 - GFF583 0.61 +0.3 318,634 - GFF583 0.62 +0.1 318,643 + GFF583 0.63 +0.6 318,673 + GFF583 0.65 +1.0 318,674 - GFF583 0.65 +0.4 318,768 + GFF583 0.72 +0.6 318,940 + GFF583 0.85 +0.6 318,941 - GFF583 0.85 +0.1 318,941 - GFF583 0.85 -0.1 318,942 + GFF583 0.85 -0.1 318,950 - GFF583 0.86 -0.1 319,049 - +0.1 319,049 - -0.3 319,050 + -0.5 319,050 + +0.3 319,087 + -0.4 319,302 + -0.7 319,481 + -0.9 319,820 + GFF584 0.31 +0.7 319,862 + GFF584 0.35 +0.3 319,867 - GFF584 0.35 +0.3 319,893 + GFF584 0.38 +0.4 319,911 - GFF584 0.39 -0.3 319,940 + GFF584 0.42 -0.1 319,941 - GFF584 0.42 -0.0 319,941 - GFF584 0.42 +0.3 319,941 - GFF584 0.42 -0.1 320,094 + GFF584 0.56 -0.3 320,105 - GFF584 0.57 +0.3 320,105 - GFF584 0.57 -0.0 320,112 + GFF584 0.57 -0.1
Or see this region's nucleotide sequence