Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4382 and GFF4383 are separated by 41 nucleotides GFF4383 and GFF4384 are separated by 69 nucleotides GFF4384 and GFF4385 are separated by 13 nucleotides GFF4385 and GFF4386 are separated by 68 nucleotides
GFF4382 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 164,746 to 168,102
GFF4382
GFF4383 - Mobile element protein, at 168,144 to 169,079
GFF4383
GFF4384 - Putative inner membrane protein, at 169,149 to 169,316
GFF4384
GFF4385 - Primosomal replication protein N prime prime, at 169,330 to 169,857
GFF4385
GFF4386 - FIG039061: hypothetical protein related to heme utilization, at 169,926 to 170,303
GFF4386
Position (kb) 168
169
170 Strain fitness (log2 ratio) -1
0
1 at 167.187 kb on - strand, within GFF4382 at 167.187 kb on - strand, within GFF4382 at 167.208 kb on + strand, within GFF4382 at 167.208 kb on + strand, within GFF4382 at 167.209 kb on - strand, within GFF4382 at 167.222 kb on - strand, within GFF4382 at 167.279 kb on + strand, within GFF4382 at 167.279 kb on + strand, within GFF4382 at 167.280 kb on - strand, within GFF4382 at 167.410 kb on + strand, within GFF4382 at 167.410 kb on + strand, within GFF4382 at 167.411 kb on - strand, within GFF4382 at 167.469 kb on + strand, within GFF4382 at 167.650 kb on - strand, within GFF4382 at 167.908 kb on + strand at 167.988 kb on + strand at 167.989 kb on - strand at 168.091 kb on - strand at 168.100 kb on + strand at 168.366 kb on - strand, within GFF4383 at 168.438 kb on - strand, within GFF4383 at 168.439 kb on + strand, within GFF4383 at 168.634 kb on + strand, within GFF4383 at 168.636 kb on + strand, within GFF4383 at 168.636 kb on + strand, within GFF4383 at 168.668 kb on + strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.669 kb on - strand, within GFF4383 at 168.672 kb on - strand, within GFF4383 at 168.680 kb on + strand, within GFF4383 at 168.681 kb on - strand, within GFF4383 at 168.716 kb on - strand, within GFF4383 at 168.887 kb on - strand, within GFF4383 at 169.183 kb on - strand, within GFF4384 at 169.205 kb on + strand, within GFF4384 at 169.205 kb on + strand, within GFF4384 at 169.224 kb on + strand, within GFF4384 at 169.270 kb on - strand, within GFF4384 at 169.270 kb on - strand, within GFF4384 at 169.316 kb on + strand at 169.317 kb on - strand at 169.352 kb on - strand at 169.403 kb on + strand, within GFF4385 at 169.404 kb on - strand, within GFF4385 at 169.404 kb on - strand, within GFF4385 at 169.837 kb on + strand at 169.838 kb on - strand at 169.892 kb on + strand at 169.935 kb on - strand at 169.937 kb on + strand at 169.937 kb on + strand at 169.969 kb on + strand, within GFF4386 at 170.078 kb on - strand, within GFF4386
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 167,187 - GFF4382 0.73 +0.8 167,187 - GFF4382 0.73 +0.5 167,208 + GFF4382 0.73 -0.7 167,208 + GFF4382 0.73 -0.4 167,209 - GFF4382 0.73 +0.7 167,222 - GFF4382 0.74 +0.6 167,279 + GFF4382 0.75 +0.3 167,279 + GFF4382 0.75 +0.4 167,280 - GFF4382 0.75 +0.2 167,410 + GFF4382 0.79 +1.3 167,410 + GFF4382 0.79 -0.0 167,411 - GFF4382 0.79 +0.4 167,469 + GFF4382 0.81 +0.3 167,650 - GFF4382 0.87 +0.3 167,908 + +0.2 167,988 + +0.2 167,989 - -0.5 168,091 - +0.0 168,100 + -0.2 168,366 - GFF4383 0.24 -0.1 168,438 - GFF4383 0.31 +0.5 168,439 + GFF4383 0.32 +0.1 168,634 + GFF4383 0.52 +0.1 168,636 + GFF4383 0.53 -0.1 168,636 + GFF4383 0.53 +1.5 168,668 + GFF4383 0.56 +0.7 168,669 - GFF4383 0.56 -0.3 168,669 - GFF4383 0.56 +0.4 168,669 - GFF4383 0.56 +0.1 168,669 - GFF4383 0.56 +0.2 168,672 - GFF4383 0.56 +0.2 168,680 + GFF4383 0.57 +0.4 168,681 - GFF4383 0.57 -0.1 168,716 - GFF4383 0.61 +0.8 168,887 - GFF4383 0.79 -0.5 169,183 - GFF4384 0.20 -0.4 169,205 + GFF4384 0.33 -0.1 169,205 + GFF4384 0.33 -0.2 169,224 + GFF4384 0.45 -0.2 169,270 - GFF4384 0.72 +0.5 169,270 - GFF4384 0.72 -0.5 169,316 + -0.0 169,317 - +0.6 169,352 - +0.5 169,403 + GFF4385 0.14 +0.1 169,404 - GFF4385 0.14 -0.3 169,404 - GFF4385 0.14 -0.0 169,837 + -0.4 169,838 - +0.4 169,892 + +0.3 169,935 - -0.1 169,937 + +0.0 169,937 + -0.6 169,969 + GFF4386 0.11 -0.2 170,078 - GFF4386 0.40 -0.1
Or see this region's nucleotide sequence