Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4300 and GFF4301 are separated by 161 nucleotides GFF4301 and GFF4302 are separated by 71 nucleotides GFF4302 and GFF4303 are separated by 209 nucleotides
GFF4300 - Proline-specific permease proY, at 84,804 to 86,234
GFF4300
GFF4301 - Maltodextrin glucosidase (EC 3.2.1.20), at 86,396 to 88,213
GFF4301
GFF4302 - Alkyl hydroperoxide reductase subunit C-like protein, at 88,285 to 88,887
GFF4302
GFF4303 - Acyl carrier protein phosphodiesterase (EC 3.1.4.14), at 89,097 to 89,678
GFF4303
Position (kb) 86
87
88
89 Strain fitness (log2 ratio) -2
-1
0
1 at 85.572 kb on - strand, within GFF4300 at 85.572 kb on - strand, within GFF4300 at 85.625 kb on + strand, within GFF4300 at 85.632 kb on - strand, within GFF4300 at 85.784 kb on + strand, within GFF4300 at 85.911 kb on + strand, within GFF4300 at 85.912 kb on - strand, within GFF4300 at 85.970 kb on - strand, within GFF4300 at 85.982 kb on + strand, within GFF4300 at 86.152 kb on - strand at 86.198 kb on + strand at 86.341 kb on + strand at 86.638 kb on + strand, within GFF4301 at 86.638 kb on + strand, within GFF4301 at 86.937 kb on + strand, within GFF4301 at 86.986 kb on - strand, within GFF4301 at 86.986 kb on - strand, within GFF4301 at 87.000 kb on + strand, within GFF4301 at 87.022 kb on + strand, within GFF4301 at 87.022 kb on + strand, within GFF4301 at 87.023 kb on - strand, within GFF4301 at 87.236 kb on + strand, within GFF4301 at 87.303 kb on - strand, within GFF4301 at 87.332 kb on + strand, within GFF4301 at 87.332 kb on + strand, within GFF4301 at 87.382 kb on + strand, within GFF4301 at 87.409 kb on - strand, within GFF4301 at 87.412 kb on + strand, within GFF4301 at 87.412 kb on + strand, within GFF4301 at 87.412 kb on + strand, within GFF4301 at 87.412 kb on + strand, within GFF4301 at 87.413 kb on - strand, within GFF4301 at 87.466 kb on + strand, within GFF4301 at 87.500 kb on + strand, within GFF4301 at 87.500 kb on + strand, within GFF4301 at 87.500 kb on + strand, within GFF4301 at 87.502 kb on + strand, within GFF4301 at 87.614 kb on - strand, within GFF4301 at 87.641 kb on - strand, within GFF4301 at 87.659 kb on - strand, within GFF4301 at 87.673 kb on + strand, within GFF4301 at 87.674 kb on - strand, within GFF4301 at 87.829 kb on + strand, within GFF4301 at 87.830 kb on - strand, within GFF4301 at 87.830 kb on - strand, within GFF4301 at 87.837 kb on - strand, within GFF4301 at 87.935 kb on + strand, within GFF4301 at 88.023 kb on + strand, within GFF4301 at 88.135 kb on + strand at 88.153 kb on + strand at 88.171 kb on + strand at 88.171 kb on + strand at 88.228 kb on + strand at 88.237 kb on - strand at 88.287 kb on - strand at 88.438 kb on - strand, within GFF4302 at 88.441 kb on - strand, within GFF4302 at 88.441 kb on - strand, within GFF4302 at 88.463 kb on - strand, within GFF4302 at 88.547 kb on + strand, within GFF4302 at 88.590 kb on - strand, within GFF4302 at 88.706 kb on + strand, within GFF4302 at 88.937 kb on + strand at 89.130 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 85,572 - GFF4300 0.54 -0.1 85,572 - GFF4300 0.54 -0.5 85,625 + GFF4300 0.57 +0.4 85,632 - GFF4300 0.58 -0.2 85,784 + GFF4300 0.68 +0.8 85,911 + GFF4300 0.77 -0.2 85,912 - GFF4300 0.77 -0.1 85,970 - GFF4300 0.81 +0.2 85,982 + GFF4300 0.82 -0.0 86,152 - +0.3 86,198 + -0.1 86,341 + -0.0 86,638 + GFF4301 0.13 +0.3 86,638 + GFF4301 0.13 +0.7 86,937 + GFF4301 0.30 -0.2 86,986 - GFF4301 0.32 -0.2 86,986 - GFF4301 0.32 +0.1 87,000 + GFF4301 0.33 +0.4 87,022 + GFF4301 0.34 +1.1 87,022 + GFF4301 0.34 +0.2 87,023 - GFF4301 0.34 -0.4 87,236 + GFF4301 0.46 +0.3 87,303 - GFF4301 0.50 +0.4 87,332 + GFF4301 0.51 +0.5 87,332 + GFF4301 0.51 +0.5 87,382 + GFF4301 0.54 +0.5 87,409 - GFF4301 0.56 +0.8 87,412 + GFF4301 0.56 -0.2 87,412 + GFF4301 0.56 -0.2 87,412 + GFF4301 0.56 +0.3 87,412 + GFF4301 0.56 +0.2 87,413 - GFF4301 0.56 +0.0 87,466 + GFF4301 0.59 +0.1 87,500 + GFF4301 0.61 -0.1 87,500 + GFF4301 0.61 +0.6 87,500 + GFF4301 0.61 -0.2 87,502 + GFF4301 0.61 +0.4 87,614 - GFF4301 0.67 -0.5 87,641 - GFF4301 0.68 -0.4 87,659 - GFF4301 0.69 +0.1 87,673 + GFF4301 0.70 +0.8 87,674 - GFF4301 0.70 -0.1 87,829 + GFF4301 0.79 -0.9 87,830 - GFF4301 0.79 +0.1 87,830 - GFF4301 0.79 +0.0 87,837 - GFF4301 0.79 -0.1 87,935 + GFF4301 0.85 -1.9 88,023 + GFF4301 0.89 +0.1 88,135 + +0.7 88,153 + +0.3 88,171 + +0.4 88,171 + -0.2 88,228 + +0.9 88,237 - -0.3 88,287 - +0.8 88,438 - GFF4302 0.25 +0.2 88,441 - GFF4302 0.26 -0.0 88,441 - GFF4302 0.26 +0.5 88,463 - GFF4302 0.30 -0.1 88,547 + GFF4302 0.43 +0.1 88,590 - GFF4302 0.51 -0.2 88,706 + GFF4302 0.70 -0.2 88,937 + +0.5 89,130 - +0.5
Or see this region's nucleotide sequence