Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF381 and GFF382 are separated by 55 nucleotides GFF382 and GFF383 are separated by 69 nucleotides GFF383 and GFF384 are separated by 5 nucleotides
GFF381 - Lipopolysaccharide core biosynthesis protein WaaP (EC 2.7.-.-), heptosyl-I-kinase, at 96,333 to 97,130
GFF381
GFF382 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,3-glucosyltransferase WaaO (EC 2.4.1.-), at 97,186 to 97,395
GFF382
GFF383 - Lipopolysaccharide 1,6-galactosyltransferase (EC 2.4.1.-), at 97,465 to 98,544
GFF383
GFF384 - UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,3-glucosyltransferase WaaO (EC 2.4.1.-), at 98,550 to 99,563
GFF384
Position (kb) 97
98
99 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 96.488 kb on + strand, within GFF381 at 96.488 kb on + strand, within GFF381 at 96.533 kb on - strand, within GFF381 at 96.533 kb on - strand, within GFF381 at 96.536 kb on + strand, within GFF381 at 96.537 kb on - strand, within GFF381 at 96.602 kb on + strand, within GFF381 at 96.662 kb on + strand, within GFF381 at 96.663 kb on - strand, within GFF381 at 96.705 kb on + strand, within GFF381 at 96.725 kb on + strand, within GFF381 at 96.776 kb on + strand, within GFF381 at 96.776 kb on + strand, within GFF381 at 98.319 kb on + strand, within GFF383 at 98.332 kb on - strand, within GFF383 at 98.723 kb on - strand, within GFF384 at 98.782 kb on + strand, within GFF384 at 99.266 kb on + strand, within GFF384 at 99.445 kb on + strand, within GFF384
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 96,488 + GFF381 0.19 -1.6 96,488 + GFF381 0.19 -0.6 96,533 - GFF381 0.25 -5.0 96,533 - GFF381 0.25 -4.8 96,536 + GFF381 0.25 -1.3 96,537 - GFF381 0.26 -1.3 96,602 + GFF381 0.34 -1.4 96,662 + GFF381 0.41 -0.5 96,663 - GFF381 0.41 -5.8 96,705 + GFF381 0.47 -1.8 96,725 + GFF381 0.49 -0.3 96,776 + GFF381 0.56 -1.2 96,776 + GFF381 0.56 -2.0 98,319 + GFF383 0.79 +0.1 98,332 - GFF383 0.80 -2.1 98,723 - GFF384 0.17 -4.5 98,782 + GFF384 0.23 -0.6 99,266 + GFF384 0.71 -4.1 99,445 + GFF384 0.88 -2.2
Or see this region's nucleotide sequence