Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3520

Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3518 and GFF3519 are separated by 142 nucleotidesGFF3519 and GFF3520 overlap by 4 nucleotidesGFF3520 and GFF3521 are separated by 701 nucleotides GFF3518 - putative outer membrane protein, at 269,171 to 270,130 GFF3518 GFF3519 - Putative inner membrane protein, at 270,273 to 270,587 GFF3519 GFF3520 - Nuclease precursor (EC 3.1.30.2), at 270,584 to 271,438 GFF3520 GFF3521 - O-antigen polymerase, at 272,140 to 273,363 GFF3521 Position (kb) 270 271 272Strain fitness (log2 ratio) -2 -1 0 1at 270.008 kb on - strand, within GFF3518at 270.381 kb on - strand, within GFF3519at 270.627 kb on + strandat 270.641 kb on - strandat 270.644 kb on - strandat 270.664 kb on + strandat 270.694 kb on + strand, within GFF3520at 270.699 kb on + strand, within GFF3520at 270.718 kb on + strand, within GFF3520at 270.719 kb on - strand, within GFF3520at 270.724 kb on + strand, within GFF3520at 270.776 kb on + strand, within GFF3520at 270.776 kb on + strand, within GFF3520at 270.776 kb on + strand, within GFF3520at 270.777 kb on - strand, within GFF3520at 270.790 kb on + strand, within GFF3520at 270.793 kb on + strand, within GFF3520at 270.935 kb on - strand, within GFF3520at 271.426 kb on - strandat 271.449 kb on - strandat 272.207 kb on - strandat 272.424 kb on - strand, within GFF3521

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
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270,008 - GFF3518 0.87 -0.1
270,381 - GFF3519 0.34 -0.9
270,627 + +0.1
270,641 - -0.2
270,644 - -0.2
270,664 + +0.7
270,694 + GFF3520 0.13 +1.3
270,699 + GFF3520 0.13 +0.3
270,718 + GFF3520 0.16 +0.2
270,719 - GFF3520 0.16 +0.2
270,724 + GFF3520 0.16 -0.0
270,776 + GFF3520 0.22 +0.2
270,776 + GFF3520 0.22 +0.7
270,776 + GFF3520 0.22 -0.3
270,777 - GFF3520 0.23 -0.2
270,790 + GFF3520 0.24 +0.7
270,793 + GFF3520 0.24 -0.1
270,935 - GFF3520 0.41 +0.5
271,426 - +0.1
271,449 - -0.1
272,207 - +0.1
272,424 - GFF3521 0.23 -1.8

Or see this region's nucleotide sequence