Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2788 and GFF2789 are separated by 18 nucleotides GFF2789 and GFF2790 overlap by 4 nucleotides GFF2790 and GFF2791 overlap by 11 nucleotides GFF2791 and GFF2792 overlap by 4 nucleotides
GFF2788 - Uptake hydrogenase large subunit (EC 1.12.99.6), at 311,212 to 313,005
GFF2788
GFF2789 - Ni,Fe-hydrogenase I cytochrome b subunit, at 313,024 to 313,755
GFF2789
GFF2790 - Hydrogenase maturation protease (EC 3.4.24.-), at 313,752 to 314,348
GFF2790
GFF2791 - Hydrogenase maturation factor hoxO, at 314,338 to 314,742
GFF2791
GFF2792 - Hydrogenase maturation factor HoxQ, at 314,739 to 315,587
GFF2792
Position (kb) 313
314 Strain fitness (log2 ratio) -2
-1
0
1 at 312.024 kb on + strand, within GFF2788 at 312.170 kb on + strand, within GFF2788 at 312.213 kb on + strand, within GFF2788 at 312.405 kb on + strand, within GFF2788 at 313.139 kb on + strand, within GFF2789 at 313.210 kb on + strand, within GFF2789 at 313.219 kb on + strand, within GFF2789 at 313.220 kb on - strand, within GFF2789 at 313.282 kb on + strand, within GFF2789 at 313.422 kb on + strand, within GFF2789 at 313.473 kb on + strand, within GFF2789 at 313.474 kb on - strand, within GFF2789 at 313.517 kb on - strand, within GFF2789 at 313.668 kb on + strand, within GFF2789 at 313.669 kb on - strand, within GFF2789 at 313.669 kb on - strand, within GFF2789 at 313.669 kb on - strand, within GFF2789 at 313.911 kb on + strand, within GFF2790 at 313.912 kb on - strand, within GFF2790 at 314.262 kb on + strand, within GFF2790 at 314.264 kb on + strand, within GFF2790 at 314.265 kb on - strand, within GFF2790 at 314.302 kb on + strand at 314.302 kb on + strand at 314.302 kb on + strand at 314.413 kb on - strand, within GFF2791 at 314.459 kb on + strand, within GFF2791 at 314.599 kb on - strand, within GFF2791 at 314.604 kb on - strand, within GFF2791 at 314.736 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 312,024 + GFF2788 0.45 -0.2 312,170 + GFF2788 0.53 -1.0 312,213 + GFF2788 0.56 -0.1 312,405 + GFF2788 0.66 +1.2 313,139 + GFF2789 0.16 +1.0 313,210 + GFF2789 0.25 -0.2 313,219 + GFF2789 0.27 +1.1 313,220 - GFF2789 0.27 +0.6 313,282 + GFF2789 0.35 +0.1 313,422 + GFF2789 0.54 -0.5 313,473 + GFF2789 0.61 +0.3 313,474 - GFF2789 0.61 -0.0 313,517 - GFF2789 0.67 -0.3 313,668 + GFF2789 0.88 +0.3 313,669 - GFF2789 0.88 -0.9 313,669 - GFF2789 0.88 +0.6 313,669 - GFF2789 0.88 +0.1 313,911 + GFF2790 0.27 -2.0 313,912 - GFF2790 0.27 +0.1 314,262 + GFF2790 0.85 +0.1 314,264 + GFF2790 0.86 -0.0 314,265 - GFF2790 0.86 +1.0 314,302 + +0.4 314,302 + -0.7 314,302 + +0.3 314,413 - GFF2791 0.19 -0.1 314,459 + GFF2791 0.30 +1.4 314,599 - GFF2791 0.64 +0.8 314,604 - GFF2791 0.66 +0.4 314,736 - +0.5
Or see this region's nucleotide sequence