Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2419 and GFF2420 are separated by 155 nucleotides GFF2420 and GFF2421 are separated by 0 nucleotides
GFF2419 - Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.99.-), at 31,397 to 33,775
GFF2419
GFF2420 - Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C, at 33,931 to 36,246
GFF2420
GFF2421 - Alpha-2-macroglobulin, at 36,247 to 41,181
GFF2421
Position (kb)
33
34
35
36
37 Strain fitness (log2 ratio)
-1
0
1 at 33.048 kb on + strand, within GFF2419 at 33.056 kb on - strand, within GFF2419 at 33.157 kb on + strand, within GFF2419 at 33.158 kb on - strand, within GFF2419 at 33.158 kb on - strand, within GFF2419 at 33.158 kb on - strand, within GFF2419 at 33.298 kb on - strand, within GFF2419 at 33.427 kb on + strand, within GFF2419 at 33.427 kb on + strand, within GFF2419 at 34.365 kb on + strand, within GFF2420 at 34.563 kb on + strand, within GFF2420 at 34.656 kb on + strand, within GFF2420 at 34.659 kb on - strand, within GFF2420 at 34.700 kb on + strand, within GFF2420 at 34.817 kb on - strand, within GFF2420 at 34.892 kb on + strand, within GFF2420 at 34.973 kb on + strand, within GFF2420 at 34.973 kb on + strand, within GFF2420 at 34.975 kb on + strand, within GFF2420 at 35.155 kb on + strand, within GFF2420 at 35.159 kb on - strand, within GFF2420 at 35.281 kb on - strand, within GFF2420 at 35.317 kb on - strand, within GFF2420 at 35.317 kb on - strand, within GFF2420 at 35.587 kb on - strand, within GFF2420 at 35.709 kb on - strand, within GFF2420 at 35.738 kb on - strand, within GFF2420 at 35.886 kb on + strand, within GFF2420 at 36.229 kb on - strand at 36.248 kb on + strand at 36.249 kb on - strand at 36.305 kb on + strand at 36.306 kb on - strand at 36.339 kb on - strand at 36.440 kb on + strand at 36.441 kb on - strand at 37.045 kb on - strand, within GFF2421 at 37.204 kb on - strand, within GFF2421
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 33,048 + GFF2419 0.69 -0.1 33,056 - GFF2419 0.70 -0.1 33,157 + GFF2419 0.74 +0.1 33,158 - GFF2419 0.74 -0.1 33,158 - GFF2419 0.74 +0.1 33,158 - GFF2419 0.74 +0.0 33,298 - GFF2419 0.80 -0.1 33,427 + GFF2419 0.85 +0.3 33,427 + GFF2419 0.85 +0.8 34,365 + GFF2420 0.19 -0.1 34,563 + GFF2420 0.27 +0.5 34,656 + GFF2420 0.31 -0.4 34,659 - GFF2420 0.31 +0.2 34,700 + GFF2420 0.33 +0.6 34,817 - GFF2420 0.38 -0.1 34,892 + GFF2420 0.41 -0.1 34,973 + GFF2420 0.45 +0.0 34,973 + GFF2420 0.45 +0.7 34,975 + GFF2420 0.45 +0.5 35,155 + GFF2420 0.53 +0.3 35,159 - GFF2420 0.53 -0.0 35,281 - GFF2420 0.58 +0.7 35,317 - GFF2420 0.60 +0.2 35,317 - GFF2420 0.60 +1.4 35,587 - GFF2420 0.72 +0.4 35,709 - GFF2420 0.77 -0.0 35,738 - GFF2420 0.78 +0.5 35,886 + GFF2420 0.84 +0.3 36,229 - -0.4 36,248 + -0.1 36,249 - +0.4 36,305 + -0.4 36,306 - -0.2 36,339 - -0.2 36,440 + -0.1 36,441 - +0.8 37,045 - GFF2421 0.16 -0.0 37,204 - GFF2421 0.19 -0.3
Or see this region's nucleotide sequence