Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1827 and GFF1828 are separated by 83 nucleotides GFF1828 and GFF1829 are separated by 114 nucleotides
GFF1827 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 9,527 to 10,585
GFF1827
GFF1828 - Thiamin biosynthesis lipoprotein ApbE, at 10,669 to 11,721
GFF1828
GFF1829 - Outer membrane protein C precursor, at 11,836 to 12,972
GFF1829
Position (kb)
10
11
12 Strain fitness (log2 ratio)
-1
0
1 at 9.912 kb on - strand, within GFF1827 at 10.095 kb on + strand, within GFF1827 at 10.095 kb on + strand, within GFF1827 at 10.096 kb on - strand, within GFF1827 at 10.099 kb on + strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.100 kb on - strand, within GFF1827 at 10.333 kb on + strand, within GFF1827 at 10.572 kb on - strand at 10.588 kb on + strand at 10.588 kb on + strand at 10.589 kb on - strand at 10.602 kb on - strand at 10.602 kb on - strand at 10.618 kb on + strand at 10.636 kb on - strand at 10.670 kb on + strand at 10.753 kb on + strand at 10.941 kb on - strand, within GFF1828 at 10.947 kb on + strand, within GFF1828 at 11.156 kb on + strand, within GFF1828 at 11.186 kb on + strand, within GFF1828 at 11.461 kb on - strand, within GFF1828 at 11.489 kb on + strand, within GFF1828 at 11.493 kb on + strand, within GFF1828 at 11.494 kb on - strand, within GFF1828 at 11.494 kb on - strand, within GFF1828 at 11.543 kb on - strand, within GFF1828 at 11.671 kb on - strand at 11.675 kb on - strand at 11.771 kb on - strand at 11.837 kb on + strand at 11.939 kb on + strand at 12.012 kb on - strand, within GFF1829 at 12.192 kb on - strand, within GFF1829 at 12.281 kb on + strand, within GFF1829 at 12.337 kb on - strand, within GFF1829 at 12.522 kb on + strand, within GFF1829 at 12.523 kb on - strand, within GFF1829 at 12.523 kb on - strand, within GFF1829 at 12.528 kb on - strand, within GFF1829 at 12.566 kb on + strand, within GFF1829 at 12.567 kb on - strand, within GFF1829 at 12.567 kb on - strand, within GFF1829 at 12.617 kb on + strand, within GFF1829
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 9,912 - GFF1827 0.36 +0.5 10,095 + GFF1827 0.54 -0.4 10,095 + GFF1827 0.54 +0.8 10,096 - GFF1827 0.54 +0.2 10,099 + GFF1827 0.54 +0.3 10,100 - GFF1827 0.54 -0.1 10,100 - GFF1827 0.54 +1.2 10,100 - GFF1827 0.54 -0.1 10,100 - GFF1827 0.54 -1.1 10,333 + GFF1827 0.76 -0.4 10,572 - +0.3 10,588 + +0.3 10,588 + +0.3 10,589 - +0.3 10,602 - -0.2 10,602 - -0.0 10,618 + -0.4 10,636 - +0.5 10,670 + -0.4 10,753 + -0.1 10,941 - GFF1828 0.26 -0.2 10,947 + GFF1828 0.26 -1.0 11,156 + GFF1828 0.46 -0.7 11,186 + GFF1828 0.49 -0.1 11,461 - GFF1828 0.75 -1.6 11,489 + GFF1828 0.78 -0.3 11,493 + GFF1828 0.78 -0.6 11,494 - GFF1828 0.78 -0.1 11,494 - GFF1828 0.78 -0.8 11,543 - GFF1828 0.83 -0.8 11,671 - -0.6 11,675 - +0.2 11,771 - +0.0 11,837 + -0.3 11,939 + -0.5 12,012 - GFF1829 0.15 -0.2 12,192 - GFF1829 0.31 -0.4 12,281 + GFF1829 0.39 -0.7 12,337 - GFF1829 0.44 +0.2 12,522 + GFF1829 0.60 +0.2 12,523 - GFF1829 0.60 +0.6 12,523 - GFF1829 0.60 +0.4 12,528 - GFF1829 0.61 +0.1 12,566 + GFF1829 0.64 +0.0 12,567 - GFF1829 0.64 +0.1 12,567 - GFF1829 0.64 -0.1 12,617 + GFF1829 0.69 -0.5
Or see this region's nucleotide sequence