Experiment: LB_plus_SM_buffer with Ffm_phage 0.01875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1328 and GFF1329 overlap by 10 nucleotides GFF1329 and GFF1330 are separated by 268 nucleotides GFF1330 and GFF1331 are separated by 122 nucleotides
GFF1328 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 37,200 to 38,036
GFF1328
GFF1329 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 38,027 to 38,197
GFF1329
GFF1330 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 38,466 to 39,644
GFF1330
GFF1331 - LysR-family transcriptional regulator STM3020, at 39,767 to 40,630
GFF1331
Position (kb)
38
39
40 Strain fitness (log2 ratio)
-1
0
1 at 37.471 kb on - strand, within GFF1328 at 37.540 kb on + strand, within GFF1328 at 37.550 kb on + strand, within GFF1328 at 37.551 kb on - strand, within GFF1328 at 37.721 kb on + strand, within GFF1328 at 37.723 kb on + strand, within GFF1328 at 37.793 kb on + strand, within GFF1328 at 37.794 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.902 kb on - strand, within GFF1328 at 37.905 kb on - strand, within GFF1328 at 37.905 kb on - strand, within GFF1328 at 37.922 kb on - strand, within GFF1328 at 37.932 kb on - strand, within GFF1328 at 37.953 kb on - strand at 38.019 kb on - strand at 38.188 kb on + strand at 38.708 kb on + strand, within GFF1330 at 38.764 kb on + strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.767 kb on - strand, within GFF1330 at 38.913 kb on - strand, within GFF1330 at 38.931 kb on + strand, within GFF1330 at 38.936 kb on + strand, within GFF1330 at 38.936 kb on + strand, within GFF1330 at 39.026 kb on + strand, within GFF1330 at 39.026 kb on + strand, within GFF1330 at 39.112 kb on + strand, within GFF1330 at 39.113 kb on - strand, within GFF1330 at 39.113 kb on - strand, within GFF1330 at 39.213 kb on + strand, within GFF1330 at 39.294 kb on - strand, within GFF1330 at 39.398 kb on + strand, within GFF1330 at 39.398 kb on + strand, within GFF1330 at 39.478 kb on - strand, within GFF1330 at 39.478 kb on - strand, within GFF1330 at 39.769 kb on - strand at 39.969 kb on - strand, within GFF1331 at 39.971 kb on + strand, within GFF1331 at 39.972 kb on - strand, within GFF1331 at 40.007 kb on - strand, within GFF1331 at 40.058 kb on - strand, within GFF1331 at 40.058 kb on - strand, within GFF1331 at 40.116 kb on + strand, within GFF1331 at 40.116 kb on + strand, within GFF1331 at 40.117 kb on - strand, within GFF1331 at 40.196 kb on + strand, within GFF1331 at 40.197 kb on - strand, within GFF1331 at 40.267 kb on + strand, within GFF1331 at 40.298 kb on + strand, within GFF1331 at 40.425 kb on + strand, within GFF1331 at 40.425 kb on + strand, within GFF1331 at 40.426 kb on - strand, within GFF1331 at 40.429 kb on + strand, within GFF1331 at 40.429 kb on + strand, within GFF1331 at 40.430 kb on - strand, within GFF1331 at 40.430 kb on - strand, within GFF1331 at 40.430 kb on - strand, within GFF1331 at 40.604 kb on + strand at 40.607 kb on + strand at 40.607 kb on + strand at 40.635 kb on + strand at 40.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.01875 MOI remove 37,471 - GFF1328 0.32 +0.4 37,540 + GFF1328 0.41 +0.0 37,550 + GFF1328 0.42 +0.5 37,551 - GFF1328 0.42 -0.1 37,721 + GFF1328 0.62 +0.1 37,723 + GFF1328 0.62 +0.1 37,793 + GFF1328 0.71 -0.9 37,794 - GFF1328 0.71 -0.6 37,845 - GFF1328 0.77 +0.1 37,845 - GFF1328 0.77 -0.1 37,845 - GFF1328 0.77 -0.2 37,902 - GFF1328 0.84 +0.0 37,905 - GFF1328 0.84 -0.2 37,905 - GFF1328 0.84 -0.1 37,922 - GFF1328 0.86 +0.7 37,932 - GFF1328 0.87 +0.1 37,953 - -0.2 38,019 - -0.0 38,188 + +0.2 38,708 + GFF1330 0.21 +0.0 38,764 + GFF1330 0.25 +1.1 38,765 - GFF1330 0.25 -0.2 38,765 - GFF1330 0.25 +0.8 38,765 - GFF1330 0.25 +0.7 38,765 - GFF1330 0.25 +0.5 38,767 - GFF1330 0.26 -0.1 38,913 - GFF1330 0.38 +0.6 38,931 + GFF1330 0.39 +0.9 38,936 + GFF1330 0.40 +0.3 38,936 + GFF1330 0.40 +0.6 39,026 + GFF1330 0.47 -0.1 39,026 + GFF1330 0.47 +0.7 39,112 + GFF1330 0.55 +0.1 39,113 - GFF1330 0.55 +0.5 39,113 - GFF1330 0.55 -0.0 39,213 + GFF1330 0.63 +1.1 39,294 - GFF1330 0.70 +0.3 39,398 + GFF1330 0.79 +0.5 39,398 + GFF1330 0.79 +0.0 39,478 - GFF1330 0.86 -0.1 39,478 - GFF1330 0.86 -0.2 39,769 - +0.2 39,969 - GFF1331 0.23 -0.1 39,971 + GFF1331 0.24 +0.2 39,972 - GFF1331 0.24 +0.2 40,007 - GFF1331 0.28 -0.0 40,058 - GFF1331 0.34 -0.3 40,058 - GFF1331 0.34 -0.5 40,116 + GFF1331 0.40 -0.2 40,116 + GFF1331 0.40 +0.6 40,117 - GFF1331 0.41 -1.2 40,196 + GFF1331 0.50 +0.1 40,197 - GFF1331 0.50 -0.1 40,267 + GFF1331 0.58 +0.2 40,298 + GFF1331 0.61 +0.5 40,425 + GFF1331 0.76 -0.3 40,425 + GFF1331 0.76 +0.2 40,426 - GFF1331 0.76 -0.5 40,429 + GFF1331 0.77 +0.3 40,429 + GFF1331 0.77 -0.1 40,430 - GFF1331 0.77 +0.2 40,430 - GFF1331 0.77 +0.2 40,430 - GFF1331 0.77 +0.1 40,604 + +0.3 40,607 + +0.3 40,607 + -0.2 40,635 + -0.2 40,639 + -0.5
Or see this region's nucleotide sequence