Experiment: 2,4-Dinitrophenol 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcX and yfcY overlap by 1 nucleotides yfcY and b2343 are separated by 180 nucleotides b2343 and fadL are separated by 371 nucleotides
b2341: yfcX - fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase (NCBI), at 2,455,037 to 2,457,181
yfcX
b2342: yfcY - acetyl-CoA acetyltransferase (NCBI), at 2,457,181 to 2,458,491
yfcY
b2343: b2343 - orf, hypothetical protein (VIMSS), at 2,458,672 to 2,458,956
b2343
b2344: fadL - long-chain fatty acid outer membrane transporter (RefSeq), at 2,459,328 to 2,460,668
fadL
Position (kb)
2457
2458
2459 Strain fitness (log2 ratio)
-1
0
1 at 2456.253 kb on + strand, within yfcX at 2456.253 kb on + strand, within yfcX at 2456.383 kb on + strand, within yfcX at 2456.402 kb on - strand, within yfcX at 2456.425 kb on + strand, within yfcX at 2456.425 kb on + strand, within yfcX at 2456.544 kb on + strand, within yfcX at 2456.614 kb on + strand, within yfcX at 2456.614 kb on + strand, within yfcX at 2456.617 kb on - strand, within yfcX at 2456.617 kb on - strand, within yfcX at 2456.663 kb on - strand, within yfcX at 2456.684 kb on + strand, within yfcX at 2456.747 kb on + strand, within yfcX at 2456.747 kb on + strand, within yfcX at 2456.771 kb on + strand, within yfcX at 2456.771 kb on + strand, within yfcX at 2457.130 kb on - strand at 2457.130 kb on - strand at 2457.241 kb on + strand at 2457.268 kb on - strand at 2457.326 kb on - strand, within yfcY at 2457.381 kb on + strand, within yfcY at 2457.473 kb on + strand, within yfcY at 2457.532 kb on + strand, within yfcY at 2457.532 kb on + strand, within yfcY at 2457.535 kb on - strand, within yfcY at 2457.539 kb on - strand, within yfcY at 2457.561 kb on + strand, within yfcY at 2457.636 kb on - strand, within yfcY at 2457.876 kb on + strand, within yfcY at 2458.221 kb on - strand, within yfcY at 2458.233 kb on - strand, within yfcY at 2458.233 kb on - strand, within yfcY at 2458.296 kb on + strand, within yfcY at 2458.452 kb on + strand at 2458.470 kb on - strand at 2458.496 kb on - strand at 2458.654 kb on + strand at 2458.859 kb on - strand, within b2343 at 2458.859 kb on - strand, within b2343 at 2459.436 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 1 mM remove 2,456,253 + yfcX b2341 0.57 -0.5 2,456,253 + yfcX b2341 0.57 +0.1 2,456,383 + yfcX b2341 0.63 +0.7 2,456,402 - yfcX b2341 0.64 +0.8 2,456,425 + yfcX b2341 0.65 +0.4 2,456,425 + yfcX b2341 0.65 -0.1 2,456,544 + yfcX b2341 0.70 -0.1 2,456,614 + yfcX b2341 0.74 +1.3 2,456,614 + yfcX b2341 0.74 -0.8 2,456,617 - yfcX b2341 0.74 -0.3 2,456,617 - yfcX b2341 0.74 -0.9 2,456,663 - yfcX b2341 0.76 +0.7 2,456,684 + yfcX b2341 0.77 +0.9 2,456,747 + yfcX b2341 0.80 -0.5 2,456,747 + yfcX b2341 0.80 -0.3 2,456,771 + yfcX b2341 0.81 +1.1 2,456,771 + yfcX b2341 0.81 +0.0 2,457,130 - -0.1 2,457,130 - +0.3 2,457,241 + -0.6 2,457,268 - -0.3 2,457,326 - yfcY b2342 0.11 +0.2 2,457,381 + yfcY b2342 0.15 -0.1 2,457,473 + yfcY b2342 0.22 +0.4 2,457,532 + yfcY b2342 0.27 +0.8 2,457,532 + yfcY b2342 0.27 +0.0 2,457,535 - yfcY b2342 0.27 +0.4 2,457,539 - yfcY b2342 0.27 +0.0 2,457,561 + yfcY b2342 0.29 -0.2 2,457,636 - yfcY b2342 0.35 +0.3 2,457,876 + yfcY b2342 0.53 +0.0 2,458,221 - yfcY b2342 0.79 -0.1 2,458,233 - yfcY b2342 0.80 -0.5 2,458,233 - yfcY b2342 0.80 +0.4 2,458,296 + yfcY b2342 0.85 +0.7 2,458,452 + -0.1 2,458,470 - -0.2 2,458,496 - +0.3 2,458,654 + -0.3 2,458,859 - b2343 b2343 0.66 -1.1 2,458,859 - b2343 b2343 0.66 -0.5 2,459,436 + -0.3
Or see this region's nucleotide sequence