Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF933 and GFF934 are separated by 55 nucleotides GFF934 and GFF935 overlap by 1 nucleotides GFF935 and GFF936 overlap by 26 nucleotides
GFF933 - Arginine/ornithine antiporter ArcD, at 109,159 to 110,562
GFF933
GFF934 - Ornithine carbamoyltransferase (EC 2.1.3.3), at 110,618 to 111,622
GFF934
GFF935 - hypothetical protein, at 111,622 to 111,759
GFF935
GFF936 - Carbamate kinase (EC 2.7.2.2), at 111,734 to 112,666
GFF936
Position (kb)
110
111
112 Strain fitness (log2 ratio)
-1
0
1 at 109.863 kb on + strand, within GFF933 at 109.924 kb on + strand, within GFF933 at 109.924 kb on + strand, within GFF933 at 110.154 kb on - strand, within GFF933 at 110.154 kb on - strand, within GFF933 at 110.154 kb on - strand, within GFF933 at 110.158 kb on - strand, within GFF933 at 110.158 kb on - strand, within GFF933 at 110.333 kb on + strand, within GFF933 at 110.348 kb on + strand, within GFF933 at 110.348 kb on + strand, within GFF933 at 110.414 kb on + strand, within GFF933 at 110.515 kb on - strand at 110.533 kb on - strand at 110.533 kb on - strand at 110.607 kb on - strand at 110.930 kb on - strand, within GFF934 at 111.138 kb on + strand, within GFF934 at 111.319 kb on - strand, within GFF934 at 111.393 kb on + strand, within GFF934 at 111.393 kb on + strand, within GFF934 at 111.735 kb on + strand at 111.736 kb on - strand at 111.967 kb on - strand, within GFF936 at 112.288 kb on + strand, within GFF936 at 112.291 kb on - strand, within GFF936 at 112.401 kb on + strand, within GFF936 at 112.401 kb on + strand, within GFF936 at 112.453 kb on + strand, within GFF936 at 112.464 kb on + strand, within GFF936 at 112.494 kb on - strand, within GFF936
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 109,863 + GFF933 0.50 +0.1 109,924 + GFF933 0.54 -0.8 109,924 + GFF933 0.54 -0.1 110,154 - GFF933 0.71 +0.2 110,154 - GFF933 0.71 +0.4 110,154 - GFF933 0.71 -0.2 110,158 - GFF933 0.71 +0.4 110,158 - GFF933 0.71 -0.1 110,333 + GFF933 0.84 -1.1 110,348 + GFF933 0.85 -0.5 110,348 + GFF933 0.85 -0.1 110,414 + GFF933 0.89 -0.3 110,515 - -0.1 110,533 - -0.4 110,533 - +0.1 110,607 - +0.1 110,930 - GFF934 0.31 +0.3 111,138 + GFF934 0.52 -1.3 111,319 - GFF934 0.70 -0.8 111,393 + GFF934 0.77 -0.6 111,393 + GFF934 0.77 -0.6 111,735 + -0.2 111,736 - +0.6 111,967 - GFF936 0.25 -0.2 112,288 + GFF936 0.59 +0.0 112,291 - GFF936 0.60 +0.0 112,401 + GFF936 0.71 +0.4 112,401 + GFF936 0.71 -0.7 112,453 + GFF936 0.77 -0.3 112,464 + GFF936 0.78 +0.1 112,494 - GFF936 0.81 -0.9
Or see this region's nucleotide sequence