Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF743 and GFF744 are separated by 375 nucleotides GFF744 and GFF745 are separated by 44 nucleotides GFF745 and GFF746 are separated by 71 nucleotides
GFF742 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 101,014 to 101,694
GFF742
GFF743 - Uncharacterized glutathione S-transferase-like protein, at 101,691 to 102,317
GFF743
GFF744 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 102,693 to 103,763
GFF744
GFF745 - Deoxyribose operon repressor, DeoR family, at 103,808 to 104,566
GFF745
GFF746 - Putative permease, at 104,638 to 105,246
GFF746
Position (kb)
102
103
104 Strain fitness (log2 ratio)
-1
0
1 at 101.807 kb on - strand, within GFF743 at 101.822 kb on + strand, within GFF743 at 101.904 kb on - strand, within GFF743 at 102.057 kb on - strand, within GFF743 at 102.127 kb on - strand, within GFF743 at 102.171 kb on - strand, within GFF743 at 102.311 kb on + strand at 102.312 kb on - strand at 102.318 kb on - strand at 102.372 kb on - strand at 102.377 kb on + strand at 102.385 kb on - strand at 102.434 kb on + strand at 102.465 kb on + strand at 102.513 kb on - strand at 102.557 kb on + strand at 102.560 kb on + strand at 102.570 kb on + strand at 102.780 kb on + strand at 102.781 kb on - strand at 102.781 kb on - strand at 102.935 kb on + strand, within GFF744 at 103.032 kb on + strand, within GFF744 at 103.812 kb on + strand at 103.823 kb on - strand at 103.880 kb on - strand at 103.899 kb on - strand, within GFF745 at 103.923 kb on - strand, within GFF745 at 103.923 kb on - strand, within GFF745 at 104.021 kb on - strand, within GFF745 at 104.192 kb on - strand, within GFF745 at 104.195 kb on - strand, within GFF745 at 104.231 kb on - strand, within GFF745 at 104.542 kb on + strand at 104.566 kb on + strand at 104.583 kb on + strand at 104.617 kb on + strand at 104.617 kb on + strand at 104.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 101,807 - GFF743 0.19 +0.1 101,822 + GFF743 0.21 +0.3 101,904 - GFF743 0.34 -0.9 102,057 - GFF743 0.58 +0.6 102,127 - GFF743 0.70 -0.3 102,171 - GFF743 0.77 +0.3 102,311 + +0.3 102,312 - -0.1 102,318 - +0.3 102,372 - +0.8 102,377 + +0.0 102,385 - -0.2 102,434 + +0.3 102,465 + +0.5 102,513 - -0.7 102,557 + -0.2 102,560 + -0.1 102,570 + +0.3 102,780 + +0.1 102,781 - -1.3 102,781 - +0.4 102,935 + GFF744 0.23 +0.4 103,032 + GFF744 0.32 +0.4 103,812 + -0.0 103,823 - +0.4 103,880 - +0.5 103,899 - GFF745 0.12 -0.5 103,923 - GFF745 0.15 +0.1 103,923 - GFF745 0.15 +0.1 104,021 - GFF745 0.28 -0.8 104,192 - GFF745 0.51 -0.3 104,195 - GFF745 0.51 -0.6 104,231 - GFF745 0.56 -0.2 104,542 + -0.2 104,566 + +0.1 104,583 + +0.1 104,617 + +0.3 104,617 + +0.1 104,618 - +0.0
Or see this region's nucleotide sequence