Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF698 and GFF699 are separated by 155 nucleotides GFF699 and GFF700 overlap by 32 nucleotides GFF700 and GFF701 are separated by 202 nucleotides GFF701 and GFF702 are separated by 204 nucleotides
GFF698 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 56,396 to 57,370
GFF698
GFF699 - probable exported protein YPO2521, at 57,526 to 57,786
GFF699
GFF700 - hypothetical protein, at 57,755 to 57,868
GFF700
GFF701 - Hypothetical Zinc-finger containing protein, at 58,071 to 58,337
GFF701
GFF702 - Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51), at 58,542 to 59,468
GFF702
Position (kb)
58
59 Strain fitness (log2 ratio)
-2
-1
0
1 at 57.150 kb on + strand, within GFF698 at 57.176 kb on + strand, within GFF698 at 57.327 kb on + strand at 57.327 kb on + strand at 57.401 kb on + strand at 57.527 kb on + strand at 57.587 kb on + strand, within GFF699 at 57.588 kb on - strand, within GFF699 at 57.771 kb on - strand at 58.047 kb on - strand at 58.156 kb on - strand, within GFF701 at 58.355 kb on - strand at 58.359 kb on + strand at 58.485 kb on - strand at 58.485 kb on - strand at 58.487 kb on - strand at 58.491 kb on - strand at 58.710 kb on - strand, within GFF702 at 58.797 kb on + strand, within GFF702 at 58.797 kb on + strand, within GFF702 at 58.799 kb on + strand, within GFF702 at 58.800 kb on - strand, within GFF702 at 58.893 kb on + strand, within GFF702 at 58.917 kb on + strand, within GFF702 at 59.064 kb on - strand, within GFF702 at 59.210 kb on - strand, within GFF702
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 57,150 + GFF698 0.77 +0.1 57,176 + GFF698 0.80 -0.2 57,327 + -0.3 57,327 + -0.6 57,401 + +0.2 57,527 + -0.2 57,587 + GFF699 0.23 -0.8 57,588 - GFF699 0.24 +0.5 57,771 - +0.5 58,047 - +0.0 58,156 - GFF701 0.32 -0.7 58,355 - -0.6 58,359 + -0.7 58,485 - -1.6 58,485 - +0.2 58,487 - +0.0 58,491 - -0.3 58,710 - GFF702 0.18 -0.6 58,797 + GFF702 0.28 -0.1 58,797 + GFF702 0.28 -0.2 58,799 + GFF702 0.28 -0.2 58,800 - GFF702 0.28 -0.2 58,893 + GFF702 0.38 -0.2 58,917 + GFF702 0.40 -0.6 59,064 - GFF702 0.56 -0.0 59,210 - GFF702 0.72 -2.0
Or see this region's nucleotide sequence