Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF633 and GFF634 overlap by 8 nucleotides GFF634 and GFF635 overlap by 8 nucleotides GFF635 and GFF636 are separated by 144 nucleotides
GFF633 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 374,410 to 375,087
GFF633
GFF634 - Phosphoglycolate phosphatase (EC 3.1.3.18), at 375,080 to 375,838
GFF634
GFF635 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 375,831 to 376,835
GFF635
GFF636 - putative outer membrane lipoprotein, at 376,980 to 377,150
GFF636
Position (kb) 375
376
377 Strain fitness (log2 ratio) -2
-1
0
1 at 375.085 kb on + strand at 375.331 kb on + strand, within GFF634 at 375.331 kb on + strand, within GFF634 at 375.331 kb on + strand, within GFF634 at 375.332 kb on - strand, within GFF634 at 375.352 kb on - strand, within GFF634 at 375.355 kb on + strand, within GFF634 at 375.356 kb on - strand, within GFF634 at 375.380 kb on + strand, within GFF634 at 375.380 kb on + strand, within GFF634 at 375.468 kb on + strand, within GFF634 at 375.537 kb on - strand, within GFF634 at 375.574 kb on + strand, within GFF634 at 375.713 kb on + strand, within GFF634 at 375.714 kb on - strand, within GFF634 at 375.719 kb on + strand, within GFF634 at 375.725 kb on + strand, within GFF634 at 375.764 kb on + strand at 375.776 kb on - strand at 375.836 kb on + strand at 375.891 kb on + strand at 375.891 kb on + strand at 375.946 kb on - strand, within GFF635 at 375.968 kb on + strand, within GFF635 at 375.998 kb on - strand, within GFF635 at 376.002 kb on - strand, within GFF635 at 376.123 kb on - strand, within GFF635 at 376.124 kb on + strand, within GFF635 at 376.124 kb on + strand, within GFF635 at 376.129 kb on - strand, within GFF635 at 376.167 kb on - strand, within GFF635 at 376.533 kb on + strand, within GFF635 at 376.874 kb on + strand at 376.875 kb on - strand at 376.886 kb on + strand at 376.886 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 375,085 + +0.2 375,331 + GFF634 0.33 -0.3 375,331 + GFF634 0.33 +1.0 375,331 + GFF634 0.33 -0.2 375,332 - GFF634 0.33 +0.7 375,352 - GFF634 0.36 -0.7 375,355 + GFF634 0.36 -0.3 375,356 - GFF634 0.36 +1.7 375,380 + GFF634 0.40 +0.1 375,380 + GFF634 0.40 -1.1 375,468 + GFF634 0.51 -0.8 375,537 - GFF634 0.60 +0.3 375,574 + GFF634 0.65 +0.5 375,713 + GFF634 0.83 +0.0 375,714 - GFF634 0.84 +0.6 375,719 + GFF634 0.84 -0.4 375,725 + GFF634 0.85 -0.2 375,764 + +0.5 375,776 - +0.1 375,836 + -0.6 375,891 + -0.8 375,891 + -1.3 375,946 - GFF635 0.11 -1.1 375,968 + GFF635 0.14 -2.2 375,998 - GFF635 0.17 -1.6 376,002 - GFF635 0.17 -2.1 376,123 - GFF635 0.29 +0.2 376,124 + GFF635 0.29 -0.3 376,124 + GFF635 0.29 -0.9 376,129 - GFF635 0.30 -1.1 376,167 - GFF635 0.33 -1.5 376,533 + GFF635 0.70 -1.7 376,874 + +0.0 376,875 - -0.2 376,886 + -0.1 376,886 + +0.0
Or see this region's nucleotide sequence