Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4439

Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4438 and GFF4439 overlap by 4 nucleotidesGFF4439 and GFF4440 are separated by 117 nucleotidesGFF4440 and GFF4441 are separated by 2 nucleotides GFF4438 - Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21), at 226,266 to 227,333 GFF4438 GFF4439 - Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), at 227,330 to 227,839 GFF4439 GFF4440 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 227,957 to 228,679 GFF4440 GFF4441 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 228,682 to 229,176 GFF4441 Position (kb) 227 228Strain fitness (log2 ratio) -1 0 1at 226.361 kb on + strandat 226.362 kb on - strandat 226.421 kb on - strand, within GFF4438at 226.435 kb on - strand, within GFF4438at 226.630 kb on - strand, within GFF4438at 226.678 kb on + strand, within GFF4438at 226.679 kb on - strand, within GFF4438at 226.883 kb on + strand, within GFF4438at 226.884 kb on - strand, within GFF4438at 226.894 kb on + strand, within GFF4438at 226.977 kb on - strand, within GFF4438at 227.527 kb on - strand, within GFF4439at 227.723 kb on + strand, within GFF4439at 227.724 kb on - strand, within GFF4439at 227.724 kb on - strand, within GFF4439at 227.894 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
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226,361 + -0.4
226,362 - +0.1
226,421 - GFF4438 0.15 -0.5
226,435 - GFF4438 0.16 +0.4
226,630 - GFF4438 0.34 -0.2
226,678 + GFF4438 0.39 -0.5
226,679 - GFF4438 0.39 +0.3
226,883 + GFF4438 0.58 +0.0
226,884 - GFF4438 0.58 -0.5
226,894 + GFF4438 0.59 -0.0
226,977 - GFF4438 0.67 +0.0
227,527 - GFF4439 0.39 +0.3
227,723 + GFF4439 0.77 -1.4
227,724 - GFF4439 0.77 -0.5
227,724 - GFF4439 0.77 -0.5
227,894 - +0.2

Or see this region's nucleotide sequence