Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF389 and GFF390 are separated by 39 nucleotides GFF390 and GFF391 overlap by 1 nucleotides GFF391 and GFF392 are separated by 2 nucleotides
GFF389 - Oligosaccharide repeat unit polymerase Wzy; O-antigen ligase, at 103,574 to 104,788
GFF389
GFF390 - Lipopolysaccharide heptosyltransferase I (EC 2.4.1.-), at 104,828 to 105,781
GFF390
GFF391 - ADP-heptose--lipooligosaccharide heptosyltransferase II (EC 2.4.1.-), at 105,781 to 106,827
GFF391
GFF392 - ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20), at 106,830 to 107,762
GFF392
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 104.947 kb on - strand, within GFF390 at 104.949 kb on - strand, within GFF390 at 104.956 kb on + strand, within GFF390 at 105.243 kb on + strand, within GFF390 at 105.322 kb on - strand, within GFF390 at 105.740 kb on + strand at 105.927 kb on + strand, within GFF391 at 105.957 kb on + strand, within GFF391 at 106.223 kb on + strand, within GFF391 at 106.686 kb on + strand, within GFF391 at 106.832 kb on - strand at 106.832 kb on - strand at 106.832 kb on - strand at 106.887 kb on - strand at 106.992 kb on - strand, within GFF392 at 107.126 kb on - strand, within GFF392 at 107.252 kb on - strand, within GFF392 at 107.404 kb on + strand, within GFF392 at 107.426 kb on - strand, within GFF392 at 107.482 kb on + strand, within GFF392 at 107.583 kb on - strand, within GFF392 at 107.781 kb on - strand at 107.822 kb on - strand at 107.827 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 104,947 - GFF390 0.12 -1.6 104,949 - GFF390 0.13 -1.7 104,956 + GFF390 0.13 -0.1 105,243 + GFF390 0.44 -1.7 105,322 - GFF390 0.52 +0.7 105,740 + -1.5 105,927 + GFF391 0.14 -0.4 105,957 + GFF391 0.17 -2.2 106,223 + GFF391 0.42 -0.5 106,686 + GFF391 0.86 +0.5 106,832 - -0.7 106,832 - -0.2 106,832 - -0.3 106,887 - +3.0 106,992 - GFF392 0.17 +0.5 107,126 - GFF392 0.32 +0.5 107,252 - GFF392 0.45 -0.1 107,404 + GFF392 0.62 -0.9 107,426 - GFF392 0.64 -0.7 107,482 + GFF392 0.70 -0.4 107,583 - GFF392 0.81 +0.1 107,781 - +0.3 107,822 - -1.2 107,827 + -0.3
Or see this region's nucleotide sequence