Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3832 and GFF3833 are separated by 98 nucleotides GFF3833 and GFF3834 overlap by 14 nucleotides GFF3834 and GFF3835 are separated by 35 nucleotides GFF3835 and GFF3836 overlap by 27 nucleotides
GFF3832 - FIG01045739: hypothetical protein, at 102,723 to 102,908
GFF3832
GFF3833 - Hydroxyethylthiazole kinase (EC 2.7.1.50), at 103,007 to 103,804
GFF3833
GFF3834 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 103,791 to 104,591
GFF3834
GFF3835 - Uncharacterized HTH-type transcriptional regulator YegW, at 104,627 to 105,373
GFF3835
GFF3836 - Uncharacterized sugar kinase YegV, PfkB family, at 105,347 to 106,312
GFF3836
Position (kb)
103
104
105 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 102.842 kb on + strand, within GFF3832 at 102.843 kb on - strand, within GFF3832 at 102.851 kb on + strand, within GFF3832 at 103.045 kb on + strand at 103.046 kb on - strand at 103.046 kb on - strand at 103.087 kb on + strand, within GFF3833 at 103.088 kb on - strand, within GFF3833 at 103.213 kb on + strand, within GFF3833 at 103.214 kb on - strand, within GFF3833 at 103.214 kb on - strand, within GFF3833 at 103.228 kb on - strand, within GFF3833 at 103.442 kb on - strand, within GFF3833 at 103.497 kb on + strand, within GFF3833 at 103.498 kb on - strand, within GFF3833 at 103.533 kb on - strand, within GFF3833 at 103.596 kb on + strand, within GFF3833 at 103.766 kb on + strand at 103.887 kb on + strand, within GFF3834 at 103.888 kb on - strand, within GFF3834 at 104.086 kb on + strand, within GFF3834 at 104.086 kb on + strand, within GFF3834 at 104.087 kb on - strand, within GFF3834 at 104.090 kb on + strand, within GFF3834 at 104.091 kb on - strand, within GFF3834 at 104.164 kb on + strand, within GFF3834 at 104.198 kb on + strand, within GFF3834 at 104.199 kb on - strand, within GFF3834 at 104.329 kb on + strand, within GFF3834 at 104.590 kb on - strand at 104.592 kb on + strand at 104.594 kb on + strand at 104.595 kb on - strand at 104.700 kb on - strand at 104.700 kb on - strand at 105.155 kb on + strand, within GFF3835 at 105.162 kb on - strand, within GFF3835 at 105.332 kb on + strand at 105.349 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 102,842 + GFF3832 0.64 +0.4 102,843 - GFF3832 0.65 -1.8 102,851 + GFF3832 0.69 -0.2 103,045 + +0.1 103,046 - -0.2 103,046 - -0.3 103,087 + GFF3833 0.10 -0.0 103,088 - GFF3833 0.10 -0.7 103,213 + GFF3833 0.26 +2.7 103,214 - GFF3833 0.26 -0.9 103,214 - GFF3833 0.26 -0.0 103,228 - GFF3833 0.28 -0.4 103,442 - GFF3833 0.55 +0.1 103,497 + GFF3833 0.61 -0.1 103,498 - GFF3833 0.62 -0.2 103,533 - GFF3833 0.66 -0.5 103,596 + GFF3833 0.74 +0.5 103,766 + +0.3 103,887 + GFF3834 0.12 -0.8 103,888 - GFF3834 0.12 -0.9 104,086 + GFF3834 0.37 +0.5 104,086 + GFF3834 0.37 -0.2 104,087 - GFF3834 0.37 +0.1 104,090 + GFF3834 0.37 -0.2 104,091 - GFF3834 0.37 +0.2 104,164 + GFF3834 0.47 -0.9 104,198 + GFF3834 0.51 -0.1 104,199 - GFF3834 0.51 -0.3 104,329 + GFF3834 0.67 +0.0 104,590 - -1.5 104,592 + -0.3 104,594 + +0.5 104,595 - +0.2 104,700 - +0.1 104,700 - -0.1 105,155 + GFF3835 0.71 -0.7 105,162 - GFF3835 0.72 +0.2 105,332 + -0.3 105,349 - -0.1
Or see this region's nucleotide sequence