Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3624 and GFF3625 are separated by 58 nucleotides GFF3625 and GFF3626 are separated by 189 nucleotides GFF3626 and GFF3627 are separated by 50 nucleotides
GFF3624 - N-ethylmaleimide reductase (EC 1.-.-.-), at 374,979 to 376,076
GFF3624
GFF3625 - Transcriptional regulator, TetR family, at 376,135 to 376,734
GFF3625
GFF3626 - FIG00553873: hypothetical protein, at 376,924 to 377,076
GFF3626
GFF3627 - Putative aldo/keto reductase, at 377,127 to 378,023
GFF3627
Position (kb) 376
377 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 375.166 kb on + strand, within GFF3624 at 375.266 kb on + strand, within GFF3624 at 375.267 kb on - strand, within GFF3624 at 375.267 kb on - strand, within GFF3624 at 375.275 kb on - strand, within GFF3624 at 375.454 kb on - strand, within GFF3624 at 375.701 kb on + strand, within GFF3624 at 375.764 kb on + strand, within GFF3624 at 375.944 kb on - strand, within GFF3624 at 376.092 kb on - strand at 376.245 kb on - strand, within GFF3625 at 376.535 kb on + strand, within GFF3625 at 376.671 kb on + strand, within GFF3625 at 376.746 kb on + strand at 376.793 kb on + strand at 377.091 kb on - strand at 377.105 kb on + strand at 377.210 kb on + strand at 377.211 kb on - strand at 377.268 kb on - strand, within GFF3627 at 377.422 kb on - strand, within GFF3627 at 377.444 kb on + strand, within GFF3627 at 377.487 kb on + strand, within GFF3627 at 377.487 kb on + strand, within GFF3627 at 377.713 kb on + strand, within GFF3627 at 377.713 kb on + strand, within GFF3627 at 377.723 kb on - strand, within GFF3627
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 375,166 + GFF3624 0.17 -0.4 375,266 + GFF3624 0.26 -0.1 375,267 - GFF3624 0.26 +0.1 375,267 - GFF3624 0.26 -0.1 375,275 - GFF3624 0.27 -0.5 375,454 - GFF3624 0.43 +0.1 375,701 + GFF3624 0.66 +0.3 375,764 + GFF3624 0.71 +0.8 375,944 - GFF3624 0.88 -0.1 376,092 - +0.1 376,245 - GFF3625 0.18 +0.2 376,535 + GFF3625 0.67 -2.2 376,671 + GFF3625 0.89 -0.2 376,746 + -0.4 376,793 + +0.4 377,091 - -0.1 377,105 + +0.3 377,210 + +0.4 377,211 - -1.3 377,268 - GFF3627 0.16 -0.5 377,422 - GFF3627 0.33 -0.5 377,444 + GFF3627 0.35 +0.0 377,487 + GFF3627 0.40 -0.4 377,487 + GFF3627 0.40 +3.4 377,713 + GFF3627 0.65 -0.0 377,713 + GFF3627 0.65 +0.2 377,723 - GFF3627 0.66 -0.2
Or see this region's nucleotide sequence