Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3038 and GFF3039 are separated by 204 nucleotides GFF3039 and GFF3040 are separated by 143 nucleotides
GFF3038 - Hexuronate utilization operon transcriptional repressor ExuR, at 52,232 to 52,981
GFF3038
GFF3039 - Putative outer membrane lipoprotein, at 53,186 to 53,917
GFF3039
GFF3040 - Biosynthetic arginine decarboxylase (EC 4.1.1.19), at 54,061 to 55,959
GFF3040
Position (kb) 53
54 Strain fitness (log2 ratio) -2
-1
0
1 at 52.381 kb on + strand, within GFF3038 at 52.382 kb on - strand, within GFF3038 at 52.429 kb on - strand, within GFF3038 at 52.607 kb on - strand, within GFF3038 at 52.609 kb on + strand, within GFF3038 at 52.609 kb on + strand, within GFF3038 at 52.610 kb on - strand, within GFF3038 at 52.624 kb on + strand, within GFF3038 at 52.624 kb on + strand, within GFF3038 at 52.670 kb on - strand, within GFF3038 at 52.722 kb on + strand, within GFF3038 at 52.771 kb on - strand, within GFF3038 at 52.820 kb on - strand, within GFF3038 at 52.944 kb on - strand at 52.987 kb on + strand at 52.987 kb on + strand at 52.987 kb on + strand at 52.991 kb on + strand at 52.991 kb on + strand at 52.992 kb on - strand at 52.992 kb on - strand at 52.992 kb on - strand at 52.992 kb on - strand at 52.995 kb on + strand at 52.996 kb on - strand at 53.026 kb on + strand at 53.094 kb on + strand at 53.094 kb on + strand at 53.318 kb on + strand, within GFF3039 at 53.393 kb on - strand, within GFF3039 at 53.443 kb on - strand, within GFF3039 at 53.579 kb on - strand, within GFF3039 at 53.965 kb on + strand at 54.052 kb on + strand at 54.155 kb on - strand at 54.204 kb on - strand at 54.384 kb on - strand, within GFF3040 at 54.426 kb on + strand, within GFF3040 at 54.429 kb on - strand, within GFF3040 at 54.429 kb on - strand, within GFF3040 at 54.429 kb on - strand, within GFF3040 at 54.459 kb on + strand, within GFF3040 at 54.459 kb on + strand, within GFF3040 at 54.460 kb on - strand, within GFF3040 at 54.462 kb on + strand, within GFF3040 at 54.462 kb on + strand, within GFF3040 at 54.810 kb on + strand, within GFF3040 at 54.915 kb on + strand, within GFF3040
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 52,381 + GFF3038 0.20 +0.6 52,382 - GFF3038 0.20 +0.9 52,429 - GFF3038 0.26 +0.1 52,607 - GFF3038 0.50 -0.7 52,609 + GFF3038 0.50 -1.1 52,609 + GFF3038 0.50 +0.0 52,610 - GFF3038 0.50 +0.2 52,624 + GFF3038 0.52 +1.1 52,624 + GFF3038 0.52 +0.3 52,670 - GFF3038 0.58 -0.3 52,722 + GFF3038 0.65 +0.1 52,771 - GFF3038 0.72 +0.9 52,820 - GFF3038 0.78 +0.6 52,944 - +0.3 52,987 + +0.2 52,987 + +0.7 52,987 + +0.6 52,991 + +0.4 52,991 + +0.7 52,992 - +0.5 52,992 - +0.5 52,992 - +0.0 52,992 - +0.1 52,995 + -0.0 52,996 - +0.4 53,026 + -0.1 53,094 + +0.7 53,094 + +0.5 53,318 + GFF3039 0.18 +0.5 53,393 - GFF3039 0.28 +0.8 53,443 - GFF3039 0.35 +0.4 53,579 - GFF3039 0.54 +0.5 53,965 + -0.0 54,052 + -0.1 54,155 - -0.6 54,204 - -0.1 54,384 - GFF3040 0.17 -1.2 54,426 + GFF3040 0.19 +0.2 54,429 - GFF3040 0.19 -0.5 54,429 - GFF3040 0.19 -1.1 54,429 - GFF3040 0.19 -1.1 54,459 + GFF3040 0.21 +0.4 54,459 + GFF3040 0.21 -0.1 54,460 - GFF3040 0.21 -0.1 54,462 + GFF3040 0.21 -0.6 54,462 + GFF3040 0.21 -1.9 54,810 + GFF3040 0.39 -0.3 54,915 + GFF3040 0.45 -0.3
Or see this region's nucleotide sequence