Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2853 and GFF2854 are separated by 18 nucleotides GFF2854 and GFF2855 are separated by 17 nucleotides GFF2855 and GFF2856 are separated by 32 nucleotides GFF2856 and GFF2857 are separated by 85 nucleotides GFF2857 and GFF2858 are separated by 73 nucleotides
GFF2853 - FIG00638765: hypothetical protein, at 375,808 to 375,942
GFF2853
GFF2854 - Ren protein, at 375,961 to 376,611
GFF2854
GFF2855 - FIG01046004: hypothetical protein, at 376,629 to 376,802
GFF2855
GFF2856 - corresponds to STY1986 from Accession AL513382: Salmonella typhi CT18, at 376,835 to 376,999
GFF2856
GFF2857 - hypothetical protein, at 377,085 to 377,210
GFF2857
GFF2858 - G-nucleotide exchange factor SopE, at 377,284 to 378,006
GFF2858
Position (kb) 376
377 Strain fitness (log2 ratio) -1
0
1 at 376.014 kb on + strand at 376.017 kb on - strand at 376.017 kb on - strand at 376.163 kb on + strand, within GFF2854 at 376.645 kb on + strand at 376.645 kb on + strand at 376.679 kb on + strand, within GFF2855 at 376.824 kb on - strand at 376.881 kb on - strand, within GFF2856 at 376.883 kb on - strand, within GFF2856 at 377.064 kb on + strand at 377.064 kb on + strand at 377.065 kb on - strand at 377.448 kb on + strand, within GFF2858 at 377.625 kb on + strand, within GFF2858
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 376,014 + +0.3 376,017 - -0.1 376,017 - +0.6 376,163 + GFF2854 0.31 +0.5 376,645 + -0.2 376,645 + +0.3 376,679 + GFF2855 0.29 +0.6 376,824 - +0.3 376,881 - GFF2856 0.28 +0.0 376,883 - GFF2856 0.29 -1.1 377,064 + -0.1 377,064 + +0.2 377,065 - +0.3 377,448 + GFF2858 0.23 -0.3 377,625 + GFF2858 0.47 -0.1
Or see this region's nucleotide sequence