Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2690 and GFF2691 overlap by 1 nucleotides GFF2691 and GFF2692 are separated by 1 nucleotides GFF2692 and GFF2693 are separated by 15 nucleotides GFF2693 and GFF2694 overlap by 13 nucleotides
GFF2690 - Peptide transport system permease protein SapC, at 211,894 to 212,784
GFF2690
GFF2691 - Peptide transport system ATP-binding protein SapD, at 212,784 to 213,776
GFF2691
GFF2692 - Peptide transport system ATP-binding protein SapF, at 213,778 to 214,584
GFF2692
GFF2693 - FIG01046389: hypothetical protein, at 214,600 to 214,725
GFF2693
GFF2694 - FIG01046389: hypothetical protein, at 214,713 to 215,309
GFF2694
Position (kb)
213
214
215 Strain fitness (log2 ratio)
-1
0
1 at 213.051 kb on - strand, within GFF2691 at 213.051 kb on - strand, within GFF2691 at 213.478 kb on + strand, within GFF2691 at 214.324 kb on - strand, within GFF2692 at 214.387 kb on + strand, within GFF2692 at 214.388 kb on - strand, within GFF2692 at 214.390 kb on - strand, within GFF2692 at 214.393 kb on + strand, within GFF2692 at 214.394 kb on - strand, within GFF2692 at 214.425 kb on + strand, within GFF2692 at 214.426 kb on - strand, within GFF2692 at 214.428 kb on + strand, within GFF2692 at 214.727 kb on + strand at 214.918 kb on - strand, within GFF2694 at 214.950 kb on + strand, within GFF2694 at 215.013 kb on + strand, within GFF2694 at 215.014 kb on - strand, within GFF2694 at 215.014 kb on - strand, within GFF2694 at 215.026 kb on - strand, within GFF2694 at 215.410 kb on + strand at 215.483 kb on + strand at 215.574 kb on + strand at 215.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 213,051 - GFF2691 0.27 -1.5 213,051 - GFF2691 0.27 -1.1 213,478 + GFF2691 0.70 -0.7 214,324 - GFF2692 0.68 -1.4 214,387 + GFF2692 0.75 -0.1 214,388 - GFF2692 0.76 -0.1 214,390 - GFF2692 0.76 -0.9 214,393 + GFF2692 0.76 -0.4 214,394 - GFF2692 0.76 -1.6 214,425 + GFF2692 0.80 -1.1 214,426 - GFF2692 0.80 +0.1 214,428 + GFF2692 0.81 -1.1 214,727 + -0.4 214,918 - GFF2694 0.34 -0.0 214,950 + GFF2694 0.40 -1.0 215,013 + GFF2694 0.50 -0.4 215,014 - GFF2694 0.50 +0.0 215,014 - GFF2694 0.50 +0.1 215,026 - GFF2694 0.52 +0.4 215,410 + -0.3 215,483 + -0.9 215,574 + -0.1 215,575 - +0.3
Or see this region's nucleotide sequence