Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2517 and GFF2518 are separated by 12 nucleotides GFF2518 and GFF2519 overlap by 13 nucleotides GFF2519 and GFF2520 are separated by 17 nucleotides GFF2520 and GFF2521 overlap by 4 nucleotides GFF2521 and GFF2522 overlap by 1 nucleotides
GFF2517 - [NiFe] hydrogenase nickel incorporation HybF-like protein, at 33,291 to 33,632
GFF2517
GFF2518 - Putative dehydrogenase protein, at 33,645 to 34,532
GFF2518
GFF2519 - Hydrogenase maturation factor HoxQ / Hydrogenase maturation factor HoxR, at 34,520 to 35,581
GFF2519
GFF2520 - Hydrogenase maturation factor hoxO, at 35,599 to 36,009
GFF2520
GFF2521 - Putative hydrogenase protein, at 36,006 to 36,305
GFF2521
GFF2522 - Hydrogenase maturation protease (EC 3.4.24.-), at 36,305 to 36,913
GFF2522
Position (kb) 34
35
36 Strain fitness (log2 ratio) -2
-1
0
1 at 33.656 kb on + strand at 33.657 kb on - strand at 33.681 kb on + strand at 33.716 kb on + strand at 33.780 kb on - strand, within GFF2518 at 33.858 kb on + strand, within GFF2518 at 33.966 kb on - strand, within GFF2518 at 34.039 kb on - strand, within GFF2518 at 34.077 kb on - strand, within GFF2518 at 34.088 kb on + strand, within GFF2518 at 34.374 kb on + strand, within GFF2518 at 34.599 kb on + strand at 34.682 kb on + strand, within GFF2519 at 34.831 kb on + strand, within GFF2519 at 34.853 kb on - strand, within GFF2519 at 34.940 kb on - strand, within GFF2519 at 35.004 kb on - strand, within GFF2519 at 35.270 kb on + strand, within GFF2519 at 35.270 kb on + strand, within GFF2519 at 35.352 kb on + strand, within GFF2519 at 35.510 kb on - strand at 35.588 kb on + strand at 35.670 kb on - strand, within GFF2520 at 35.682 kb on + strand, within GFF2520 at 35.682 kb on + strand, within GFF2520 at 35.683 kb on - strand, within GFF2520 at 35.684 kb on + strand, within GFF2520 at 35.685 kb on - strand, within GFF2520 at 35.685 kb on - strand, within GFF2520 at 35.686 kb on + strand, within GFF2520 at 35.687 kb on - strand, within GFF2520 at 35.806 kb on - strand, within GFF2520 at 35.807 kb on + strand, within GFF2520 at 35.807 kb on + strand, within GFF2520 at 35.808 kb on - strand, within GFF2520 at 35.808 kb on - strand, within GFF2520 at 36.299 kb on + strand at 36.402 kb on + strand, within GFF2522 at 36.402 kb on + strand, within GFF2522 at 36.403 kb on - strand, within GFF2522
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 33,656 + +0.4 33,657 - -0.3 33,681 + +0.3 33,716 + -0.1 33,780 - GFF2518 0.15 +0.2 33,858 + GFF2518 0.24 +0.0 33,966 - GFF2518 0.36 +0.8 34,039 - GFF2518 0.44 +1.1 34,077 - GFF2518 0.49 -0.0 34,088 + GFF2518 0.50 -0.2 34,374 + GFF2518 0.82 -0.5 34,599 + -0.2 34,682 + GFF2519 0.15 +0.6 34,831 + GFF2519 0.29 +0.1 34,853 - GFF2519 0.31 +0.2 34,940 - GFF2519 0.40 +0.3 35,004 - GFF2519 0.46 +0.5 35,270 + GFF2519 0.71 +0.6 35,270 + GFF2519 0.71 +0.1 35,352 + GFF2519 0.78 +0.3 35,510 - -1.0 35,588 + +0.1 35,670 - GFF2520 0.17 +0.6 35,682 + GFF2520 0.20 +0.9 35,682 + GFF2520 0.20 +0.4 35,683 - GFF2520 0.20 -0.2 35,684 + GFF2520 0.21 -0.5 35,685 - GFF2520 0.21 -0.1 35,685 - GFF2520 0.21 +0.2 35,686 + GFF2520 0.21 -0.2 35,687 - GFF2520 0.21 -0.1 35,806 - GFF2520 0.50 -0.4 35,807 + GFF2520 0.51 -0.2 35,807 + GFF2520 0.51 +0.2 35,808 - GFF2520 0.51 -0.1 35,808 - GFF2520 0.51 +0.1 36,299 + -1.2 36,402 + GFF2522 0.16 -1.8 36,402 + GFF2522 0.16 +0.1 36,403 - GFF2522 0.16 -0.0
Or see this region's nucleotide sequence