Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2229 and GFF2230 are separated by 156 nucleotides GFF2230 and GFF2231 are separated by 67 nucleotides
GFF2229 - FIG074102: hypothetical protein, at 22,929 to 23,777
GFF2229
GFF2230 - Outer membrane sugar transport protein YshA, at 23,934 to 24,626
GFF2230
GFF2231 - Glucuronide transport protein YihO, at 24,694 to 26,115
GFF2231
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-1
0
1 at 22.939 kb on + strand at 22.940 kb on - strand at 22.954 kb on + strand at 22.954 kb on + strand at 22.955 kb on - strand at 22.959 kb on - strand at 22.969 kb on + strand at 23.099 kb on + strand, within GFF2229 at 23.100 kb on - strand, within GFF2229 at 23.162 kb on + strand, within GFF2229 at 23.178 kb on - strand, within GFF2229 at 23.181 kb on - strand, within GFF2229 at 23.379 kb on + strand, within GFF2229 at 23.385 kb on - strand, within GFF2229 at 23.436 kb on + strand, within GFF2229 at 23.437 kb on - strand, within GFF2229 at 23.437 kb on - strand, within GFF2229 at 23.619 kb on - strand, within GFF2229 at 23.624 kb on + strand, within GFF2229 at 23.624 kb on + strand, within GFF2229 at 23.627 kb on + strand, within GFF2229 at 23.627 kb on + strand, within GFF2229 at 23.667 kb on - strand, within GFF2229 at 23.760 kb on - strand at 23.805 kb on - strand at 24.236 kb on - strand, within GFF2230 at 24.451 kb on + strand, within GFF2230 at 24.510 kb on - strand, within GFF2230 at 24.586 kb on - strand at 24.911 kb on - strand, within GFF2231 at 24.943 kb on - strand, within GFF2231 at 24.994 kb on - strand, within GFF2231 at 25.113 kb on + strand, within GFF2231 at 25.126 kb on - strand, within GFF2231 at 25.203 kb on - strand, within GFF2231 at 25.247 kb on - strand, within GFF2231 at 25.356 kb on + strand, within GFF2231 at 25.439 kb on - strand, within GFF2231 at 25.475 kb on + strand, within GFF2231 at 25.476 kb on - strand, within GFF2231 at 25.553 kb on + strand, within GFF2231 at 25.553 kb on + strand, within GFF2231 at 25.554 kb on - strand, within GFF2231 at 25.554 kb on - strand, within GFF2231 at 25.576 kb on - strand, within GFF2231 at 25.603 kb on + strand, within GFF2231 at 25.604 kb on - strand, within GFF2231 at 25.608 kb on + strand, within GFF2231 at 25.609 kb on - strand, within GFF2231
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 22,939 + -0.0 22,940 - +0.3 22,954 + +0.1 22,954 + -0.4 22,955 - +0.0 22,959 - -0.1 22,969 + +0.2 23,099 + GFF2229 0.20 -0.2 23,100 - GFF2229 0.20 +0.1 23,162 + GFF2229 0.27 +1.4 23,178 - GFF2229 0.29 -0.2 23,181 - GFF2229 0.30 +0.1 23,379 + GFF2229 0.53 -0.2 23,385 - GFF2229 0.54 -0.1 23,436 + GFF2229 0.60 -0.1 23,437 - GFF2229 0.60 +0.1 23,437 - GFF2229 0.60 -0.4 23,619 - GFF2229 0.81 +0.1 23,624 + GFF2229 0.82 -0.1 23,624 + GFF2229 0.82 -0.1 23,627 + GFF2229 0.82 +0.8 23,627 + GFF2229 0.82 -0.2 23,667 - GFF2229 0.87 +0.3 23,760 - -0.1 23,805 - +0.8 24,236 - GFF2230 0.44 -0.6 24,451 + GFF2230 0.75 -1.1 24,510 - GFF2230 0.83 -0.3 24,586 - +0.4 24,911 - GFF2231 0.15 +0.0 24,943 - GFF2231 0.18 +0.4 24,994 - GFF2231 0.21 -0.5 25,113 + GFF2231 0.29 +0.1 25,126 - GFF2231 0.30 +0.0 25,203 - GFF2231 0.36 +0.1 25,247 - GFF2231 0.39 -0.1 25,356 + GFF2231 0.47 +0.4 25,439 - GFF2231 0.52 -0.2 25,475 + GFF2231 0.55 -0.0 25,476 - GFF2231 0.55 -0.2 25,553 + GFF2231 0.60 +0.5 25,553 + GFF2231 0.60 -0.5 25,554 - GFF2231 0.60 -0.0 25,554 - GFF2231 0.60 -0.1 25,576 - GFF2231 0.62 -1.6 25,603 + GFF2231 0.64 +0.7 25,604 - GFF2231 0.64 +0.2 25,608 + GFF2231 0.64 -0.1 25,609 - GFF2231 0.64 +0.1
Or see this region's nucleotide sequence