Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2103 and GFF2104 are separated by 17 nucleotides GFF2104 and GFF2105 are separated by 61 nucleotides GFF2105 and GFF2106 overlap by 16 nucleotides
GFF2103 - LysR family transcriptional regulator YfiE, at 11,530 to 12,429
GFF2103
GFF2104 - ATP-dependent RNA helicase SrmB, at 12,447 to 13,781
GFF2104
GFF2105 - tRNA (adenine37-N(6))-methyltransferase TrmN6 (EC 2.1.1.223), at 13,843 to 14,649
GFF2105
GFF2106 - L-aspartate oxidase (EC 1.4.3.16), at 14,634 to 16,256
GFF2106
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1 at 11.564 kb on + strand at 11.914 kb on + strand, within GFF2103 at 11.973 kb on - strand, within GFF2103 at 12.079 kb on - strand, within GFF2103 at 12.190 kb on - strand, within GFF2103 at 12.201 kb on + strand, within GFF2103 at 12.240 kb on - strand, within GFF2103 at 12.267 kb on + strand, within GFF2103 at 12.371 kb on + strand at 12.375 kb on + strand at 12.375 kb on + strand at 12.375 kb on + strand at 12.376 kb on - strand at 12.442 kb on + strand at 12.442 kb on + strand at 12.669 kb on + strand, within GFF2104 at 12.909 kb on - strand, within GFF2104 at 12.912 kb on - strand, within GFF2104 at 13.434 kb on - strand, within GFF2104 at 13.434 kb on - strand, within GFF2104 at 13.651 kb on - strand at 13.810 kb on + strand at 13.910 kb on - strand at 14.046 kb on - strand, within GFF2105 at 14.125 kb on - strand, within GFF2105 at 14.266 kb on - strand, within GFF2105 at 14.268 kb on + strand, within GFF2105 at 14.429 kb on + strand, within GFF2105 at 14.600 kb on - strand at 14.613 kb on + strand at 14.635 kb on + strand at 14.635 kb on + strand at 14.639 kb on + strand at 14.702 kb on - strand at 14.711 kb on + strand at 14.736 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 11,564 + -0.0 11,914 + GFF2103 0.43 -1.1 11,973 - GFF2103 0.49 +1.2 12,079 - GFF2103 0.61 +0.0 12,190 - GFF2103 0.73 -0.2 12,201 + GFF2103 0.75 -0.2 12,240 - GFF2103 0.79 +0.5 12,267 + GFF2103 0.82 -0.2 12,371 + +0.2 12,375 + -0.1 12,375 + +0.5 12,375 + +0.2 12,376 - -0.1 12,442 + -0.2 12,442 + +0.3 12,669 + GFF2104 0.17 +0.0 12,909 - GFF2104 0.35 -0.9 12,912 - GFF2104 0.35 -0.6 13,434 - GFF2104 0.74 -0.8 13,434 - GFF2104 0.74 +0.2 13,651 - -0.1 13,810 + +0.8 13,910 - +0.3 14,046 - GFF2105 0.25 -0.1 14,125 - GFF2105 0.35 +0.3 14,266 - GFF2105 0.52 +0.2 14,268 + GFF2105 0.53 -0.0 14,429 + GFF2105 0.73 +0.2 14,600 - -0.1 14,613 + +0.2 14,635 + -0.3 14,635 + -0.8 14,639 + -0.4 14,702 - -0.2 14,711 + -0.5 14,736 - -0.7
Or see this region's nucleotide sequence