Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2043 and GFF2044 are separated by 163 nucleotides GFF2044 and GFF2045 are separated by 118 nucleotides GFF2045 and GFF2046 are separated by 139 nucleotides GFF2046 and GFF2047 are separated by 48 nucleotides
GFF2043 - Sulfate transporter, CysZ-type, at 10,223 to 11,086
GFF2043
GFF2044 - Cysteine synthase (EC 2.5.1.47), at 11,250 to 12,221
GFF2044
GFF2045 - hypothetical protein, at 12,340 to 12,465
GFF2045
GFF2046 - Phosphotransferase system, phosphocarrier protein HPr, at 12,605 to 12,862
GFF2046
GFF2047 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 12,911 to 14,638
GFF2047
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-2
-1
0
1 at 10.272 kb on + strand at 10.313 kb on + strand, within GFF2043 at 10.324 kb on + strand, within GFF2043 at 10.367 kb on + strand, within GFF2043 at 10.368 kb on - strand, within GFF2043 at 10.420 kb on + strand, within GFF2043 at 10.498 kb on - strand, within GFF2043 at 10.544 kb on + strand, within GFF2043 at 10.545 kb on - strand, within GFF2043 at 10.545 kb on - strand, within GFF2043 at 10.545 kb on - strand, within GFF2043 at 10.691 kb on - strand, within GFF2043 at 10.720 kb on - strand, within GFF2043 at 10.775 kb on - strand, within GFF2043 at 10.982 kb on - strand, within GFF2043 at 10.999 kb on + strand, within GFF2043 at 10.999 kb on + strand, within GFF2043 at 10.999 kb on + strand, within GFF2043 at 11.011 kb on + strand at 11.148 kb on - strand at 11.206 kb on + strand at 11.206 kb on + strand at 11.484 kb on + strand, within GFF2044 at 11.485 kb on - strand, within GFF2044 at 11.569 kb on + strand, within GFF2044 at 11.569 kb on + strand, within GFF2044 at 11.570 kb on - strand, within GFF2044 at 11.814 kb on - strand, within GFF2044 at 11.858 kb on - strand, within GFF2044 at 11.925 kb on - strand, within GFF2044 at 12.014 kb on + strand, within GFF2044 at 12.014 kb on + strand, within GFF2044 at 12.197 kb on + strand at 12.364 kb on - strand, within GFF2045 at 12.465 kb on + strand at 12.465 kb on + strand at 12.533 kb on + strand at 13.138 kb on + strand, within GFF2047 at 13.139 kb on - strand, within GFF2047 at 13.139 kb on - strand, within GFF2047
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 10,272 + +0.5 10,313 + GFF2043 0.10 -0.4 10,324 + GFF2043 0.12 +0.6 10,367 + GFF2043 0.17 -0.2 10,368 - GFF2043 0.17 +0.0 10,420 + GFF2043 0.23 +0.2 10,498 - GFF2043 0.32 +0.7 10,544 + GFF2043 0.37 +0.6 10,545 - GFF2043 0.37 +0.4 10,545 - GFF2043 0.37 -1.2 10,545 - GFF2043 0.37 +0.4 10,691 - GFF2043 0.54 -0.6 10,720 - GFF2043 0.58 -0.8 10,775 - GFF2043 0.64 -0.2 10,982 - GFF2043 0.88 +0.3 10,999 + GFF2043 0.90 -0.0 10,999 + GFF2043 0.90 -0.8 10,999 + GFF2043 0.90 -0.2 11,011 + -0.1 11,148 - -0.1 11,206 + +0.5 11,206 + -0.8 11,484 + GFF2044 0.24 -0.6 11,485 - GFF2044 0.24 -0.3 11,569 + GFF2044 0.33 -0.3 11,569 + GFF2044 0.33 -0.6 11,570 - GFF2044 0.33 -0.1 11,814 - GFF2044 0.58 -0.4 11,858 - GFF2044 0.63 +0.5 11,925 - GFF2044 0.69 -0.1 12,014 + GFF2044 0.79 -0.2 12,014 + GFF2044 0.79 -0.5 12,197 + +0.0 12,364 - GFF2045 0.19 -0.3 12,465 + +0.3 12,465 + -0.1 12,533 + -0.3 13,138 + GFF2047 0.13 -1.5 13,139 - GFF2047 0.13 -1.4 13,139 - GFF2047 0.13 -2.6
Or see this region's nucleotide sequence