Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1877 and GFF1878 are separated by 110 nucleotides GFF1878 and GFF1879 are separated by 657 nucleotides GFF1879 and GFF1880 are separated by 6 nucleotides
GFF1877 - Von Willebrand factor type A domain protein, at 68,078 to 69,859
GFF1877
GFF1878 - hypothetical protein, at 69,970 to 70,089
GFF1878
GFF1879 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 70,747 to 72,204
GFF1879
GFF1880 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 72,211 to 73,740
GFF1880
Position (kb) 70
71
72
73 Strain fitness (log2 ratio) -2
-1
0
1 at 70.091 kb on - strand at 70.126 kb on + strand at 70.198 kb on - strand at 70.234 kb on - strand at 70.515 kb on + strand at 70.634 kb on - strand at 70.907 kb on + strand, within GFF1879 at 70.908 kb on - strand, within GFF1879 at 70.908 kb on - strand, within GFF1879 at 70.931 kb on + strand, within GFF1879 at 70.932 kb on - strand, within GFF1879 at 70.935 kb on - strand, within GFF1879 at 70.937 kb on + strand, within GFF1879 at 70.938 kb on - strand, within GFF1879 at 70.960 kb on + strand, within GFF1879 at 70.961 kb on - strand, within GFF1879 at 71.015 kb on + strand, within GFF1879 at 71.016 kb on - strand, within GFF1879 at 71.049 kb on + strand, within GFF1879 at 71.126 kb on + strand, within GFF1879 at 71.208 kb on - strand, within GFF1879 at 71.299 kb on - strand, within GFF1879 at 71.365 kb on - strand, within GFF1879 at 71.488 kb on - strand, within GFF1879 at 71.513 kb on + strand, within GFF1879 at 71.616 kb on + strand, within GFF1879 at 71.616 kb on + strand, within GFF1879 at 71.673 kb on - strand, within GFF1879 at 71.673 kb on - strand, within GFF1879 at 71.729 kb on + strand, within GFF1879 at 71.730 kb on - strand, within GFF1879 at 71.888 kb on + strand, within GFF1879 at 71.889 kb on - strand, within GFF1879 at 71.979 kb on - strand, within GFF1879 at 72.225 kb on + strand at 72.226 kb on - strand at 72.300 kb on - strand at 72.342 kb on + strand at 72.411 kb on - strand, within GFF1880 at 72.439 kb on + strand, within GFF1880 at 72.452 kb on - strand, within GFF1880 at 72.452 kb on - strand, within GFF1880 at 72.624 kb on + strand, within GFF1880 at 72.640 kb on - strand, within GFF1880 at 72.653 kb on + strand, within GFF1880 at 72.654 kb on - strand, within GFF1880 at 72.749 kb on + strand, within GFF1880 at 72.790 kb on - strand, within GFF1880 at 72.790 kb on - strand, within GFF1880 at 72.791 kb on + strand, within GFF1880 at 72.792 kb on - strand, within GFF1880 at 72.871 kb on - strand, within GFF1880 at 72.871 kb on - strand, within GFF1880
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 70,091 - -0.0 70,126 + +1.3 70,198 - +0.2 70,234 - -0.1 70,515 + +0.1 70,634 - +0.5 70,907 + GFF1879 0.11 -1.4 70,908 - GFF1879 0.11 -1.9 70,908 - GFF1879 0.11 -1.3 70,931 + GFF1879 0.13 -1.1 70,932 - GFF1879 0.13 -2.1 70,935 - GFF1879 0.13 -0.0 70,937 + GFF1879 0.13 -0.5 70,938 - GFF1879 0.13 +0.5 70,960 + GFF1879 0.15 -1.0 70,961 - GFF1879 0.15 -1.9 71,015 + GFF1879 0.18 -0.8 71,016 - GFF1879 0.18 +0.4 71,049 + GFF1879 0.21 +0.4 71,126 + GFF1879 0.26 -0.9 71,208 - GFF1879 0.32 -2.3 71,299 - GFF1879 0.38 +1.0 71,365 - GFF1879 0.42 -1.6 71,488 - GFF1879 0.51 -0.8 71,513 + GFF1879 0.53 -2.0 71,616 + GFF1879 0.60 -0.6 71,616 + GFF1879 0.60 -2.2 71,673 - GFF1879 0.64 -1.5 71,673 - GFF1879 0.64 -0.0 71,729 + GFF1879 0.67 -1.3 71,730 - GFF1879 0.67 -1.4 71,888 + GFF1879 0.78 -2.3 71,889 - GFF1879 0.78 -1.0 71,979 - GFF1879 0.84 -1.2 72,225 + -2.2 72,226 - -0.7 72,300 - -1.2 72,342 + -0.7 72,411 - GFF1880 0.13 -0.9 72,439 + GFF1880 0.15 -1.5 72,452 - GFF1880 0.16 -1.3 72,452 - GFF1880 0.16 -1.2 72,624 + GFF1880 0.27 -1.5 72,640 - GFF1880 0.28 -1.3 72,653 + GFF1880 0.29 -0.1 72,654 - GFF1880 0.29 -1.8 72,749 + GFF1880 0.35 -1.3 72,790 - GFF1880 0.38 -1.4 72,790 - GFF1880 0.38 -0.5 72,791 + GFF1880 0.38 -0.8 72,792 - GFF1880 0.38 -1.4 72,871 - GFF1880 0.43 -1.0 72,871 - GFF1880 0.43 -0.2
Or see this region's nucleotide sequence