Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1246 and GFF1247 are separated by 344 nucleotides GFF1247 and GFF1248 are separated by 444 nucleotides GFF1248 and GFF1249 are separated by 69 nucleotides
GFF1246 - RlgA, at 76,978 to 77,400
GFF1246
GFF1247 - Carbonic anhydrase (EC 4.2.1.1), at 77,745 to 78,485
GFF1247
GFF1248 - hypothetical protein, at 78,930 to 79,070
GFF1248
GFF1249 - FIG074102: hypothetical protein, at 79,140 to 79,442
GFF1249
Position (kb) 77
78
79 Strain fitness (log2 ratio) -1
0
1
2 at 76.869 kb on - strand at 76.873 kb on + strand at 76.873 kb on + strand at 76.873 kb on + strand at 76.947 kb on + strand at 76.947 kb on + strand at 77.012 kb on + strand at 77.076 kb on + strand, within GFF1246 at 77.078 kb on + strand, within GFF1246 at 77.078 kb on + strand, within GFF1246 at 77.079 kb on - strand, within GFF1246 at 77.079 kb on - strand, within GFF1246 at 77.087 kb on + strand, within GFF1246 at 77.088 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.105 kb on - strand, within GFF1246 at 77.105 kb on - strand, within GFF1246 at 77.127 kb on - strand, within GFF1246 at 77.128 kb on + strand, within GFF1246 at 77.215 kb on - strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.301 kb on - strand, within GFF1246 at 77.301 kb on - strand, within GFF1246 at 77.365 kb on - strand at 77.449 kb on - strand at 77.491 kb on - strand at 77.528 kb on + strand at 77.529 kb on - strand at 77.539 kb on - strand at 77.567 kb on - strand at 77.700 kb on + strand at 77.747 kb on - strand at 77.786 kb on - strand at 77.789 kb on + strand at 77.790 kb on - strand at 77.916 kb on - strand, within GFF1247 at 77.922 kb on - strand, within GFF1247 at 78.002 kb on + strand, within GFF1247 at 78.003 kb on - strand, within GFF1247 at 78.102 kb on - strand, within GFF1247 at 78.117 kb on - strand, within GFF1247 at 78.201 kb on - strand, within GFF1247 at 78.305 kb on + strand, within GFF1247 at 78.423 kb on + strand at 78.486 kb on + strand at 78.487 kb on - strand at 78.496 kb on + strand at 78.501 kb on - strand at 78.508 kb on + strand at 78.509 kb on - strand at 78.527 kb on + strand at 78.529 kb on + strand at 78.529 kb on + strand at 78.529 kb on + strand at 78.530 kb on - strand at 78.530 kb on - strand at 78.533 kb on + strand at 78.534 kb on - strand at 78.534 kb on - strand at 78.534 kb on - strand at 78.534 kb on - strand at 78.581 kb on + strand at 78.585 kb on + strand at 78.754 kb on + strand at 78.812 kb on + strand at 78.845 kb on + strand at 78.846 kb on - strand at 78.846 kb on - strand at 78.846 kb on - strand at 78.911 kb on + strand at 78.911 kb on + strand at 78.912 kb on - strand at 78.912 kb on - strand at 78.912 kb on - strand at 79.028 kb on + strand, within GFF1248 at 79.080 kb on - strand at 79.094 kb on - strand at 79.141 kb on + strand at 79.142 kb on - strand at 79.142 kb on - strand at 79.181 kb on - strand, within GFF1249 at 79.269 kb on - strand, within GFF1249 at 79.269 kb on - strand, within GFF1249 at 79.323 kb on - strand, within GFF1249 at 79.380 kb on + strand, within GFF1249 at 79.381 kb on - strand, within GFF1249 at 79.394 kb on + strand, within GFF1249 at 79.394 kb on + strand, within GFF1249 at 79.394 kb on + strand, within GFF1249 at 79.436 kb on - strand at 79.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 76,869 - +0.1 76,873 + +0.4 76,873 + -0.4 76,873 + +0.4 76,947 + +0.2 76,947 + +0.5 77,012 + +1.0 77,076 + GFF1246 0.23 -0.3 77,078 + GFF1246 0.24 +0.4 77,078 + GFF1246 0.24 +0.1 77,079 - GFF1246 0.24 -0.2 77,079 - GFF1246 0.24 +0.4 77,087 + GFF1246 0.26 -0.0 77,088 - GFF1246 0.26 +0.5 77,090 - GFF1246 0.26 +0.0 77,090 - GFF1246 0.26 +0.4 77,090 - GFF1246 0.26 -0.0 77,105 - GFF1246 0.30 -0.2 77,105 - GFF1246 0.30 +0.5 77,127 - GFF1246 0.35 -0.2 77,128 + GFF1246 0.35 -0.2 77,215 - GFF1246 0.56 -1.1 77,300 + GFF1246 0.76 +0.0 77,300 + GFF1246 0.76 +0.1 77,300 + GFF1246 0.76 +0.1 77,301 - GFF1246 0.76 +0.1 77,301 - GFF1246 0.76 +0.2 77,365 - +0.4 77,449 - +0.0 77,491 - +1.4 77,528 + -0.5 77,529 - -0.1 77,539 - +0.5 77,567 - +0.4 77,700 + +0.4 77,747 - +0.2 77,786 - +0.1 77,789 + +0.3 77,790 - +0.6 77,916 - GFF1247 0.23 -0.3 77,922 - GFF1247 0.24 +0.2 78,002 + GFF1247 0.35 +0.8 78,003 - GFF1247 0.35 -0.0 78,102 - GFF1247 0.48 +0.4 78,117 - GFF1247 0.50 +0.4 78,201 - GFF1247 0.62 +0.7 78,305 + GFF1247 0.76 +0.2 78,423 + +1.8 78,486 + +0.1 78,487 - +0.6 78,496 + -0.1 78,501 - +0.8 78,508 + -1.3 78,509 - +0.5 78,527 + -0.3 78,529 + +0.1 78,529 + +0.5 78,529 + +0.0 78,530 - +0.8 78,530 - +0.1 78,533 + -0.3 78,534 - +0.5 78,534 - -0.7 78,534 - -0.1 78,534 - +0.6 78,581 + -0.1 78,585 + -0.1 78,754 + -0.8 78,812 + +0.1 78,845 + -0.1 78,846 - +0.2 78,846 - -0.5 78,846 - -0.4 78,911 + +0.1 78,911 + -0.2 78,912 - +0.5 78,912 - -0.2 78,912 - +0.3 79,028 + GFF1248 0.70 +0.2 79,080 - -0.0 79,094 - +0.3 79,141 + +0.9 79,142 - -0.2 79,142 - -0.7 79,181 - GFF1249 0.14 -0.2 79,269 - GFF1249 0.43 +0.3 79,269 - GFF1249 0.43 -0.1 79,323 - GFF1249 0.60 +0.1 79,380 + GFF1249 0.79 -1.4 79,381 - GFF1249 0.80 -0.1 79,394 + GFF1249 0.84 +0.0 79,394 + GFF1249 0.84 +0.3 79,394 + GFF1249 0.84 -1.2 79,436 - +0.1 79,447 + +0.6
Or see this region's nucleotide sequence