Experiment: LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1062 and GFF1063 are separated by 48 nucleotides GFF1063 and GFF1064 overlap by 4 nucleotides GFF1064 and GFF1065 are separated by 196 nucleotides GFF1065 and GFF1066 are separated by 12 nucleotides
GFF1062 - Inosine/xanthosine triphosphatase (EC 3.6.1.-), at 244,810 to 245,325
GFF1062
GFF1063 - Phosphoglycerate mutase (EC 5.4.2.1), at 245,374 to 246,021
GFF1063
GFF1064 - Right origin-binding protein, at 246,018 to 246,887
GFF1064
GFF1065 - Conserved uncharacterized protein CreA, at 247,084 to 247,572
GFF1065
GFF1066 - Two-component response regulator CreB, at 247,585 to 248,274
GFF1066
Position (kb) 246
247 Strain fitness (log2 ratio) -1
0
1 at 245.146 kb on - strand, within GFF1062 at 245.230 kb on - strand, within GFF1062 at 245.233 kb on - strand, within GFF1062 at 245.386 kb on + strand at 245.523 kb on + strand, within GFF1063 at 245.540 kb on + strand, within GFF1063 at 245.856 kb on - strand, within GFF1063 at 245.856 kb on - strand, within GFF1063 at 246.012 kb on - strand at 246.097 kb on + strand at 246.106 kb on - strand, within GFF1064 at 246.106 kb on - strand, within GFF1064 at 246.107 kb on + strand, within GFF1064 at 246.146 kb on + strand, within GFF1064 at 246.202 kb on + strand, within GFF1064 at 246.217 kb on + strand, within GFF1064 at 246.296 kb on - strand, within GFF1064 at 246.301 kb on - strand, within GFF1064 at 246.352 kb on + strand, within GFF1064 at 246.368 kb on + strand, within GFF1064 at 246.369 kb on - strand, within GFF1064 at 246.455 kb on - strand, within GFF1064 at 246.455 kb on - strand, within GFF1064 at 246.483 kb on - strand, within GFF1064 at 246.731 kb on - strand, within GFF1064 at 247.191 kb on + strand, within GFF1065 at 247.213 kb on - strand, within GFF1065 at 247.292 kb on + strand, within GFF1065 at 247.462 kb on + strand, within GFF1065 at 247.462 kb on + strand, within GFF1065 at 247.498 kb on + strand, within GFF1065 at 247.645 kb on + strand at 247.885 kb on + strand, within GFF1066 at 247.886 kb on - strand, within GFF1066
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.0001875 MOI remove 245,146 - GFF1062 0.65 -0.1 245,230 - GFF1062 0.81 +0.6 245,233 - GFF1062 0.82 +1.6 245,386 + -0.1 245,523 + GFF1063 0.23 -0.1 245,540 + GFF1063 0.26 +0.1 245,856 - GFF1063 0.74 +1.1 245,856 - GFF1063 0.74 -0.7 246,012 - +0.0 246,097 + +0.4 246,106 - GFF1064 0.10 -0.0 246,106 - GFF1064 0.10 -0.4 246,107 + GFF1064 0.10 -1.2 246,146 + GFF1064 0.15 +0.0 246,202 + GFF1064 0.21 +0.1 246,217 + GFF1064 0.23 -0.5 246,296 - GFF1064 0.32 -0.1 246,301 - GFF1064 0.33 +0.8 246,352 + GFF1064 0.38 +0.5 246,368 + GFF1064 0.40 +0.1 246,369 - GFF1064 0.40 +0.2 246,455 - GFF1064 0.50 -0.3 246,455 - GFF1064 0.50 -0.6 246,483 - GFF1064 0.53 -0.2 246,731 - GFF1064 0.82 +0.4 247,191 + GFF1065 0.22 +1.2 247,213 - GFF1065 0.26 -0.4 247,292 + GFF1065 0.43 +0.4 247,462 + GFF1065 0.77 +0.1 247,462 + GFF1065 0.77 -0.6 247,498 + GFF1065 0.85 +0.8 247,645 + +0.5 247,885 + GFF1066 0.43 -0.1 247,886 - GFF1066 0.44 +0.7
Or see this region's nucleotide sequence