Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cydB and ybgT are separated by 14 nucleotides ybgT and ybgE overlap by 1 nucleotides ybgE and ybgC are separated by 149 nucleotides ybgC and tolQ overlap by 4 nucleotides
b0734: cydB - cytochrome d terminal oxidase, subunit II (NCBI), at 772,265 to 773,404
cydB
b4515: ybgT - hypothetical protein (NCBI), at 773,419 to 773,532
ybgT
b0735: ybgE - conserved inner membrane protein (NCBI), at 773,532 to 773,825
ybgE
b0736: ybgC - predicted acyl-CoA thioesterase (NCBI), at 773,975 to 774,379
ybgC
b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068
tolQ
Position (kb) 773
774 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 773.380 kb on + strand at 773.486 kb on + strand, within ybgT at 773.572 kb on + strand, within ybgE at 773.617 kb on - strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.693 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.702 kb on + strand, within ybgE at 773.857 kb on + strand at 773.857 kb on + strand at 773.900 kb on - strand at 773.900 kb on - strand at 773.968 kb on + strand at 773.974 kb on + strand at 773.974 kb on + strand at 774.007 kb on + strand at 774.007 kb on + strand at 774.103 kb on + strand, within ybgC at 774.103 kb on + strand, within ybgC at 774.256 kb on + strand, within ybgC at 774.265 kb on - strand, within ybgC at 774.299 kb on - strand, within ybgC at 774.336 kb on + strand, within ybgC at 774.390 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 773,380 + -1.3 773,486 + ybgT b4515 0.59 -1.1 773,572 + ybgE b0735 0.14 -1.2 773,617 - ybgE b0735 0.29 -0.1 773,693 + ybgE b0735 0.55 -0.2 773,693 + ybgE b0735 0.55 -0.1 773,702 + ybgE b0735 0.58 -0.6 773,702 + ybgE b0735 0.58 -0.3 773,857 + -0.1 773,857 + -1.9 773,900 - -0.2 773,900 - +0.3 773,968 + -1.1 773,974 + -0.5 773,974 + -0.7 774,007 + +0.1 774,007 + -1.2 774,103 + ybgC b0736 0.32 +0.3 774,103 + ybgC b0736 0.32 -0.3 774,256 + ybgC b0736 0.69 -3.7 774,265 - ybgC b0736 0.72 -1.0 774,299 - ybgC b0736 0.80 -2.3 774,336 + ybgC b0736 0.89 -0.7 774,390 - +0.4
Or see this region's nucleotide sequence