Strain Fitness in Escherichia coli BW25113 around b2133

Experiment: 2,4-Dinitrophenol 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbglX and dld are separated by 195 nucleotidesdld and pbpG are separated by 37 nucleotides b2132: bglX - beta-D-glucoside glucohydrolase, periplasmic (NCBI), at 2,217,714 to 2,220,011 bglX b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922 dld b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892 pbpG Position (kb) 2220 2221 2222Strain fitness (log2 ratio) -2 -1 0 1 2at 2219.307 kb on - strand, within bglXat 2219.343 kb on + strand, within bglXat 2219.398 kb on + strand, within bglXat 2219.398 kb on + strand, within bglXat 2219.407 kb on + strand, within bglXat 2219.415 kb on - strand, within bglXat 2219.539 kb on - strand, within bglXat 2219.559 kb on - strand, within bglXat 2219.659 kb on - strand, within bglXat 2219.664 kb on - strand, within bglXat 2219.891 kb on + strandat 2219.891 kb on + strandat 2219.969 kb on + strandat 2219.969 kb on + strandat 2219.977 kb on - strandat 2219.978 kb on - strandat 2219.998 kb on - strandat 2220.036 kb on - strandat 2220.147 kb on + strandat 2220.147 kb on + strandat 2220.167 kb on - strandat 2220.167 kb on - strandat 2220.206 kb on - strandat 2220.206 kb on - strandat 2220.220 kb on - strandat 2220.276 kb on - strandat 2220.276 kb on - strandat 2220.279 kb on - strandat 2220.330 kb on + strandat 2220.560 kb on - strand, within dldat 2220.623 kb on + strand, within dldat 2220.635 kb on + strand, within dldat 2220.651 kb on - strand, within dldat 2220.781 kb on + strand, within dldat 2220.781 kb on + strand, within dldat 2220.784 kb on - strand, within dldat 2220.784 kb on - strand, within dldat 2220.811 kb on + strand, within dldat 2221.087 kb on + strand, within dldat 2221.199 kb on + strand, within dldat 2221.199 kb on + strand, within dldat 2221.333 kb on + strand, within dldat 2221.333 kb on + strand, within dldat 2221.373 kb on + strand, within dldat 2221.373 kb on + strand, within dldat 2221.482 kb on - strand, within dldat 2221.505 kb on - strand, within dldat 2221.577 kb on + strand, within dldat 2221.658 kb on - strand, within dldat 2221.687 kb on + strand, within dldat 2221.697 kb on + strand, within dldat 2221.697 kb on + strand, within dldat 2221.730 kb on - strand, within dldat 2221.780 kb on + strandat 2221.781 kb on - strandat 2221.938 kb on - strandat 2222.020 kb on + strandat 2222.020 kb on + strandat 2222.154 kb on + strand, within pbpGat 2222.163 kb on + strand, within pbpGat 2222.171 kb on - strand, within pbpGat 2222.183 kb on + strand, within pbpGat 2222.254 kb on - strand, within pbpGat 2222.306 kb on + strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.314 kb on - strand, within pbpGat 2222.552 kb on - strand, within pbpGat 2222.552 kb on - strand, within pbpGat 2222.602 kb on - strand, within pbpGat 2222.804 kb on - strandat 2222.806 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM
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2,219,307 - bglX b2132 0.69 +0.3
2,219,343 + bglX b2132 0.71 +1.7
2,219,398 + bglX b2132 0.73 +0.1
2,219,398 + bglX b2132 0.73 +0.5
2,219,407 + bglX b2132 0.74 +0.5
2,219,415 - bglX b2132 0.74 +1.5
2,219,539 - bglX b2132 0.79 +0.6
2,219,559 - bglX b2132 0.80 +0.3
2,219,659 - bglX b2132 0.85 -0.3
2,219,664 - bglX b2132 0.85 +0.1
2,219,891 + +0.7
2,219,891 + +0.5
2,219,969 + -0.8
2,219,969 + -0.2
2,219,977 - -0.1
2,219,978 - -0.4
2,219,998 - +1.0
2,220,036 - -0.3
2,220,147 + -1.6
2,220,147 + +0.9
2,220,167 - -1.4
2,220,167 - +0.6
2,220,206 - -1.3
2,220,206 - -0.4
2,220,220 - -0.8
2,220,276 - -0.4
2,220,276 - -1.0
2,220,279 - -1.2
2,220,330 + -0.8
2,220,560 - dld b2133 0.21 -0.8
2,220,623 + dld b2133 0.24 -0.9
2,220,635 + dld b2133 0.25 -0.1
2,220,651 - dld b2133 0.26 -1.1
2,220,781 + dld b2133 0.33 -0.7
2,220,781 + dld b2133 0.33 -0.2
2,220,784 - dld b2133 0.34 +0.2
2,220,784 - dld b2133 0.34 +0.8
2,220,811 + dld b2133 0.35 +1.3
2,221,087 + dld b2133 0.51 -0.4
2,221,199 + dld b2133 0.58 -0.7
2,221,199 + dld b2133 0.58 -0.6
2,221,333 + dld b2133 0.66 -0.8
2,221,333 + dld b2133 0.66 -2.0
2,221,373 + dld b2133 0.68 -0.4
2,221,373 + dld b2133 0.68 +0.8
2,221,482 - dld b2133 0.74 +0.2
2,221,505 - dld b2133 0.76 -0.9
2,221,577 + dld b2133 0.80 -0.4
2,221,658 - dld b2133 0.85 -0.5
2,221,687 + dld b2133 0.86 -0.4
2,221,697 + dld b2133 0.87 -0.5
2,221,697 + dld b2133 0.87 -0.7
2,221,730 - dld b2133 0.89 -0.7
2,221,780 + -0.9
2,221,781 - -1.4
2,221,938 - +0.6
2,222,020 + -0.0
2,222,020 + -0.2
2,222,154 + pbpG b2134 0.21 +0.2
2,222,163 + pbpG b2134 0.22 +0.1
2,222,171 - pbpG b2134 0.23 +0.6
2,222,183 + pbpG b2134 0.24 +0.2
2,222,254 - pbpG b2134 0.32 -0.6
2,222,306 + pbpG b2134 0.37 +0.4
2,222,314 - pbpG b2134 0.38 -0.4
2,222,314 - pbpG b2134 0.38 +0.1
2,222,314 - pbpG b2134 0.38 +0.5
2,222,314 - pbpG b2134 0.38 +0.5
2,222,552 - pbpG b2134 0.63 +0.3
2,222,552 - pbpG b2134 0.63 +0.6
2,222,602 - pbpG b2134 0.69 +0.7
2,222,804 - +2.0
2,222,806 - +0.4

Or see this region's nucleotide sequence