Strain Fitness in Escherichia coli BW25113 around b1991

Experiment: 2,4-Dinitrophenol 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nterfK and cobT are separated by 64 nucleotidescobT and cobS are separated by 11 nucleotidescobS and cobU overlap by 4 nucleotides b1990: erfK - conserved protein with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,060,415 to 2,061,347 erfK b1991: cobT - nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (NCBI), at 2,061,412 to 2,062,491 cobT b1992: cobS - cobalamin synthase (NCBI), at 2,062,503 to 2,063,246 cobS b1993: cobU - adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI), at 2,063,243 to 2,063,788 cobU Position (kb) 2061 2062 2063Strain fitness (log2 ratio) -2 -1 0 1at 2060.520 kb on + strand, within erfKat 2060.520 kb on + strand, within erfKat 2060.530 kb on - strand, within erfKat 2060.536 kb on + strand, within erfKat 2060.573 kb on + strand, within erfKat 2060.610 kb on - strand, within erfKat 2060.630 kb on - strand, within erfKat 2060.630 kb on - strand, within erfKat 2060.715 kb on + strand, within erfKat 2060.730 kb on + strand, within erfKat 2060.730 kb on - strand, within erfKat 2060.743 kb on - strand, within erfKat 2060.743 kb on - strand, within erfKat 2060.821 kb on - strand, within erfKat 2060.976 kb on - strand, within erfKat 2060.976 kb on - strand, within erfKat 2060.983 kb on + strand, within erfKat 2060.989 kb on - strand, within erfKat 2061.007 kb on - strand, within erfKat 2061.078 kb on - strand, within erfKat 2061.200 kb on - strand, within erfKat 2061.211 kb on - strand, within erfKat 2061.216 kb on - strand, within erfKat 2061.365 kb on - strandat 2061.365 kb on - strandat 2061.426 kb on - strandat 2061.426 kb on - strandat 2061.517 kb on + strandat 2061.734 kb on + strand, within cobTat 2061.734 kb on + strand, within cobTat 2061.871 kb on + strand, within cobTat 2061.871 kb on + strand, within cobTat 2061.890 kb on - strand, within cobTat 2061.955 kb on - strand, within cobTat 2061.955 kb on - strand, within cobTat 2061.974 kb on + strand, within cobTat 2062.013 kb on - strand, within cobTat 2062.105 kb on + strand, within cobTat 2062.134 kb on - strand, within cobTat 2062.134 kb on - strand, within cobTat 2062.238 kb on - strand, within cobTat 2062.238 kb on - strand, within cobTat 2062.333 kb on + strand, within cobTat 2062.333 kb on + strand, within cobTat 2062.357 kb on - strand, within cobTat 2062.416 kb on - strandat 2062.704 kb on + strand, within cobSat 2063.068 kb on + strand, within cobSat 2063.182 kb on + strandat 2063.182 kb on - strandat 2063.203 kb on - strandat 2063.378 kb on - strand, within cobUat 2063.378 kb on - strand, within cobUat 2063.390 kb on + strand, within cobU

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Per-strain Table

Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM
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2,060,520 + erfK b1990 0.11 -0.1
2,060,520 + erfK b1990 0.11 +0.2
2,060,530 - erfK b1990 0.12 +0.5
2,060,536 + erfK b1990 0.13 +0.5
2,060,573 + erfK b1990 0.17 +0.4
2,060,610 - erfK b1990 0.21 +0.4
2,060,630 - erfK b1990 0.23 -0.6
2,060,630 - erfK b1990 0.23 -0.3
2,060,715 + erfK b1990 0.32 -0.0
2,060,730 + erfK b1990 0.34 +0.2
2,060,730 - erfK b1990 0.34 -1.0
2,060,743 - erfK b1990 0.35 +0.2
2,060,743 - erfK b1990 0.35 +0.3
2,060,821 - erfK b1990 0.44 +0.6
2,060,976 - erfK b1990 0.60 +0.4
2,060,976 - erfK b1990 0.60 +0.4
2,060,983 + erfK b1990 0.61 -0.3
2,060,989 - erfK b1990 0.62 +0.5
2,061,007 - erfK b1990 0.63 -0.5
2,061,078 - erfK b1990 0.71 -0.6
2,061,200 - erfK b1990 0.84 -0.6
2,061,211 - erfK b1990 0.85 +0.5
2,061,216 - erfK b1990 0.86 +0.3
2,061,365 - +0.0
2,061,365 - +0.7
2,061,426 - -0.2
2,061,426 - +0.4
2,061,517 + +0.2
2,061,734 + cobT b1991 0.30 +0.0
2,061,734 + cobT b1991 0.30 +0.0
2,061,871 + cobT b1991 0.42 +0.3
2,061,871 + cobT b1991 0.42 +0.1
2,061,890 - cobT b1991 0.44 -0.4
2,061,955 - cobT b1991 0.50 -1.3
2,061,955 - cobT b1991 0.50 +1.0
2,061,974 + cobT b1991 0.52 +0.6
2,062,013 - cobT b1991 0.56 -0.5
2,062,105 + cobT b1991 0.64 +0.3
2,062,134 - cobT b1991 0.67 +0.6
2,062,134 - cobT b1991 0.67 -0.4
2,062,238 - cobT b1991 0.76 +0.1
2,062,238 - cobT b1991 0.76 +0.2
2,062,333 + cobT b1991 0.85 -0.1
2,062,333 + cobT b1991 0.85 +0.1
2,062,357 - cobT b1991 0.88 +0.3
2,062,416 - -2.5
2,062,704 + cobS b1992 0.27 -0.4
2,063,068 + cobS b1992 0.76 -0.2
2,063,182 + -0.2
2,063,182 - -0.1
2,063,203 - +1.1
2,063,378 - cobU b1993 0.25 +0.6
2,063,378 - cobU b1993 0.25 +0.7
2,063,390 + cobU b1993 0.27 -0.3

Or see this region's nucleotide sequence