Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepT and ycfD are separated by 48 nucleotides ycfD and phoQ are separated by 75 nucleotides
b1127: pepT - peptidase T (NCBI), at 1,185,067 to 1,186,293
pepT
b1128: ycfD - orf, hypothetical protein (VIMSS), at 1,186,342 to 1,187,463
ycfD
b1129: phoQ - sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI), at 1,187,539 to 1,188,999
phoQ
Position (kb)
1186
1187
1188 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1185.409 kb on + strand, within pepT at 1185.639 kb on + strand, within pepT at 1185.647 kb on + strand, within pepT at 1185.720 kb on + strand, within pepT at 1185.746 kb on - strand, within pepT at 1185.779 kb on - strand, within pepT at 1185.886 kb on + strand, within pepT at 1185.986 kb on - strand, within pepT at 1186.009 kb on + strand, within pepT at 1186.182 kb on - strand at 1186.468 kb on - strand, within ycfD at 1186.507 kb on - strand, within ycfD at 1186.533 kb on - strand, within ycfD at 1186.778 kb on + strand, within ycfD at 1186.778 kb on + strand, within ycfD at 1186.805 kb on - strand, within ycfD at 1186.876 kb on - strand, within ycfD at 1186.942 kb on - strand, within ycfD at 1186.950 kb on + strand, within ycfD at 1186.950 kb on + strand, within ycfD at 1187.202 kb on + strand, within ycfD at 1187.292 kb on + strand, within ycfD at 1187.292 kb on + strand, within ycfD at 1187.349 kb on + strand, within ycfD at 1187.349 kb on + strand, within ycfD at 1187.446 kb on + strand at 1187.521 kb on + strand at 1187.672 kb on - strand at 1187.672 kb on - strand at 1187.674 kb on - strand at 1187.674 kb on - strand at 1187.685 kb on + strand, within phoQ at 1187.775 kb on - strand, within phoQ at 1187.776 kb on - strand, within phoQ at 1187.776 kb on - strand, within phoQ at 1187.779 kb on + strand, within phoQ at 1187.844 kb on - strand, within phoQ at 1187.955 kb on + strand, within phoQ at 1187.973 kb on - strand, within phoQ at 1188.100 kb on - strand, within phoQ at 1188.191 kb on + strand, within phoQ at 1188.246 kb on + strand, within phoQ at 1188.320 kb on + strand, within phoQ at 1188.342 kb on + strand, within phoQ at 1188.342 kb on + strand, within phoQ at 1188.435 kb on - strand, within phoQ at 1188.435 kb on - strand, within phoQ at 1188.461 kb on + strand, within phoQ
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 1,185,409 + pepT b1127 0.28 +0.4 1,185,639 + pepT b1127 0.47 +0.1 1,185,647 + pepT b1127 0.47 -0.5 1,185,720 + pepT b1127 0.53 +0.0 1,185,746 - pepT b1127 0.55 +0.3 1,185,779 - pepT b1127 0.58 +0.7 1,185,886 + pepT b1127 0.67 -0.6 1,185,986 - pepT b1127 0.75 +0.1 1,186,009 + pepT b1127 0.77 +0.4 1,186,182 - -0.1 1,186,468 - ycfD b1128 0.11 -1.2 1,186,507 - ycfD b1128 0.15 -1.0 1,186,533 - ycfD b1128 0.17 -0.6 1,186,778 + ycfD b1128 0.39 +0.0 1,186,778 + ycfD b1128 0.39 -1.2 1,186,805 - ycfD b1128 0.41 -0.2 1,186,876 - ycfD b1128 0.48 -0.4 1,186,942 - ycfD b1128 0.53 -1.1 1,186,950 + ycfD b1128 0.54 -1.3 1,186,950 + ycfD b1128 0.54 -1.5 1,187,202 + ycfD b1128 0.77 -0.2 1,187,292 + ycfD b1128 0.85 -0.9 1,187,292 + ycfD b1128 0.85 -2.3 1,187,349 + ycfD b1128 0.90 -1.3 1,187,349 + ycfD b1128 0.90 -1.1 1,187,446 + -1.0 1,187,521 + +0.6 1,187,672 - -2.6 1,187,672 - +0.1 1,187,674 - -1.6 1,187,674 - -3.4 1,187,685 + phoQ b1129 0.10 -0.1 1,187,775 - phoQ b1129 0.16 -1.7 1,187,776 - phoQ b1129 0.16 -2.7 1,187,776 - phoQ b1129 0.16 -1.9 1,187,779 + phoQ b1129 0.16 -0.9 1,187,844 - phoQ b1129 0.21 -3.4 1,187,955 + phoQ b1129 0.28 -0.8 1,187,973 - phoQ b1129 0.30 -4.5 1,188,100 - phoQ b1129 0.38 -2.2 1,188,191 + phoQ b1129 0.45 -2.4 1,188,246 + phoQ b1129 0.48 -0.9 1,188,320 + phoQ b1129 0.53 -3.6 1,188,342 + phoQ b1129 0.55 -2.0 1,188,342 + phoQ b1129 0.55 -1.6 1,188,435 - phoQ b1129 0.61 -2.7 1,188,435 - phoQ b1129 0.61 -3.1 1,188,461 + phoQ b1129 0.63 -2.3
Or see this region's nucleotide sequence