Experiment: 2,4-Dinitrophenol 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagC and nagA are separated by 8 nucleotides nagA and nagB are separated by 59 nucleotides
b0676: nagC - DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI), at 699,597 to 700,817
nagC
b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974
nagA
b0678: nagB - glucosamine-6-phosphate deaminase (NCBI), at 702,034 to 702,834
nagB
Position (kb) 700
701
702 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 699.844 kb on + strand, within nagC at 699.854 kb on + strand, within nagC at 699.939 kb on + strand, within nagC at 699.966 kb on - strand, within nagC at 699.966 kb on - strand, within nagC at 699.968 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.132 kb on - strand, within nagC at 700.132 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.269 kb on + strand, within nagC at 700.454 kb on + strand, within nagC at 700.486 kb on + strand, within nagC at 700.542 kb on - strand, within nagC at 700.726 kb on - strand at 700.726 kb on - strand at 700.733 kb on + strand at 700.759 kb on + strand at 700.759 kb on + strand at 700.761 kb on + strand at 700.813 kb on - strand at 700.813 kb on - strand at 700.967 kb on - strand, within nagA at 700.977 kb on - strand, within nagA at 701.025 kb on - strand, within nagA at 701.074 kb on - strand, within nagA at 701.191 kb on + strand, within nagA at 701.244 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.321 kb on + strand, within nagA at 701.327 kb on - strand, within nagA at 701.347 kb on - strand, within nagA at 701.382 kb on + strand, within nagA at 701.417 kb on + strand, within nagA at 701.498 kb on - strand, within nagA at 701.531 kb on - strand, within nagA at 701.565 kb on + strand, within nagA at 701.565 kb on + strand, within nagA at 701.648 kb on + strand, within nagA at 701.692 kb on + strand, within nagA at 701.694 kb on - strand, within nagA at 701.755 kb on + strand, within nagA at 701.766 kb on + strand, within nagA at 701.805 kb on - strand, within nagA at 701.829 kb on + strand, within nagA at 701.904 kb on - strand at 701.904 kb on - strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.968 kb on + strand at 701.980 kb on - strand at 702.001 kb on + strand at 702.001 kb on + strand at 702.011 kb on - strand at 702.020 kb on - strand at 702.104 kb on + strand at 702.160 kb on - strand, within nagB at 702.160 kb on - strand, within nagB at 702.166 kb on + strand, within nagB at 702.180 kb on - strand, within nagB at 702.217 kb on - strand, within nagB at 702.250 kb on + strand, within nagB at 702.346 kb on + strand, within nagB at 702.619 kb on - strand, within nagB at 702.624 kb on + strand, within nagB at 702.624 kb on + strand, within nagB at 702.769 kb on + strand at 702.794 kb on + strand at 702.804 kb on + strand at 702.823 kb on + strand at 702.823 kb on + strand at 702.881 kb on + strand at 702.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM remove 699,844 + nagC b0676 0.20 -1.9 699,854 + nagC b0676 0.21 -2.7 699,939 + nagC b0676 0.28 -1.1 699,966 - nagC b0676 0.30 -1.4 699,966 - nagC b0676 0.30 -2.8 699,968 + nagC b0676 0.30 -1.9 700,083 + nagC b0676 0.40 -1.9 700,083 + nagC b0676 0.40 -3.4 700,132 - nagC b0676 0.44 -0.7 700,132 - nagC b0676 0.44 -3.1 700,193 - nagC b0676 0.49 -0.3 700,193 - nagC b0676 0.49 -1.7 700,193 - nagC b0676 0.49 -2.9 700,206 - nagC b0676 0.50 +0.3 700,206 - nagC b0676 0.50 -0.1 700,269 + nagC b0676 0.55 -1.8 700,454 + nagC b0676 0.70 -2.4 700,486 + nagC b0676 0.73 -1.2 700,542 - nagC b0676 0.77 -2.3 700,726 - +2.5 700,726 - -1.5 700,733 + -3.3 700,759 + -1.9 700,759 + -3.7 700,761 + -1.5 700,813 - -2.1 700,813 - -1.1 700,967 - nagA b0677 0.12 -2.1 700,977 - nagA b0677 0.13 -2.7 701,025 - nagA b0677 0.17 -1.3 701,074 - nagA b0677 0.22 -0.2 701,191 + nagA b0677 0.32 -0.3 701,244 - nagA b0677 0.36 -2.2 701,309 - nagA b0677 0.42 -0.8 701,309 - nagA b0677 0.42 -1.2 701,321 + nagA b0677 0.43 -2.8 701,327 - nagA b0677 0.44 -0.6 701,347 - nagA b0677 0.45 -2.1 701,382 + nagA b0677 0.48 -2.9 701,417 + nagA b0677 0.51 -1.9 701,498 - nagA b0677 0.58 -3.2 701,531 - nagA b0677 0.61 -2.2 701,565 + nagA b0677 0.64 -1.7 701,565 + nagA b0677 0.64 -3.2 701,648 + nagA b0677 0.72 -1.8 701,692 + nagA b0677 0.75 -0.7 701,694 - nagA b0677 0.76 -1.7 701,755 + nagA b0677 0.81 -1.2 701,766 + nagA b0677 0.82 -0.3 701,805 - nagA b0677 0.85 -2.6 701,829 + nagA b0677 0.87 -2.3 701,904 - -2.5 701,904 - -3.0 701,959 + -1.7 701,959 + -1.5 701,959 + -2.3 701,968 + -3.4 701,980 - -0.2 702,001 + -1.1 702,001 + -0.7 702,011 - -0.5 702,020 - +0.5 702,104 + -3.2 702,160 - nagB b0678 0.16 +0.6 702,160 - nagB b0678 0.16 +1.5 702,166 + nagB b0678 0.16 -1.4 702,180 - nagB b0678 0.18 -0.2 702,217 - nagB b0678 0.23 -0.2 702,250 + nagB b0678 0.27 -2.8 702,346 + nagB b0678 0.39 -1.9 702,619 - nagB b0678 0.73 +0.5 702,624 + nagB b0678 0.74 -2.2 702,624 + nagB b0678 0.74 -3.0 702,769 + -3.2 702,794 + -4.4 702,804 + -2.9 702,823 + -2.7 702,823 + -2.7 702,881 + -3.0 702,966 + +0.2
Or see this region's nucleotide sequence