Strain Fitness in Escherichia coli BW25113 around b0474

Experiment: 2,4-Dinitrophenol 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthtpG and adk are separated by 180 nucleotidesadk and hemH are separated by 235 nucleotides b0473: htpG - heat shock protein 90 (NCBI), at 494,344 to 496,218 htpG b0474: adk - adenylate kinase (NCBI), at 496,399 to 497,043 adk b0475: hemH - ferrochelatase (NCBI), at 497,279 to 498,241 hemH Position (kb) 496 497 498Strain fitness (log2 ratio) -2 -1 0 1at 495.497 kb on - strand, within htpGat 495.526 kb on - strand, within htpGat 495.622 kb on - strand, within htpGat 495.643 kb on + strand, within htpGat 495.643 kb on + strand, within htpGat 495.651 kb on - strand, within htpGat 495.653 kb on + strand, within htpGat 495.661 kb on + strand, within htpGat 495.661 kb on + strand, within htpGat 495.763 kb on - strand, within htpGat 495.783 kb on - strand, within htpGat 495.968 kb on + strand, within htpGat 496.118 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2,4-Dinitrophenol 2 mM
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495,497 - htpG b0473 0.61 +0.1
495,526 - htpG b0473 0.63 -1.3
495,622 - htpG b0473 0.68 -0.6
495,643 + htpG b0473 0.69 +0.3
495,643 + htpG b0473 0.69 -1.0
495,651 - htpG b0473 0.70 -0.0
495,653 + htpG b0473 0.70 +0.4
495,661 + htpG b0473 0.70 -1.2
495,661 + htpG b0473 0.70 -0.5
495,763 - htpG b0473 0.76 -0.3
495,783 - htpG b0473 0.77 -1.0
495,968 + htpG b0473 0.87 -2.3
496,118 + -0.1

Or see this region's nucleotide sequence