Experiment: L-Lysine 60 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00410 and PS417_00415 are separated by 192 nucleotides PS417_00415 and PS417_00420 are separated by 34 nucleotides PS417_00420 and PS417_00425 are separated by 168 nucleotides
PS417_00410: PS417_00410 - GTP-binding protein, at 81,025 to 81,666
_00410
PS417_00415: PS417_00415 - cytochrome, at 81,859 to 82,161
_00415
PS417_00420: PS417_00420 - cytochrome C, at 82,196 to 82,822
_00420
PS417_00425: PS417_00425 - thiol:disulfide interchange protein, at 82,991 to 83,626
_00425
Position (kb) 81
82
83 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 80.911 kb on + strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.912 kb on - strand at 80.912 kb on - strand at 80.912 kb on - strand at 80.921 kb on + strand at 80.921 kb on + strand at 80.921 kb on + strand at 80.936 kb on - strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.742 kb on + strand at 81.809 kb on + strand at 81.809 kb on + strand at 81.937 kb on + strand, within PS417_00415 at 82.159 kb on + strand at 82.159 kb on + strand at 82.159 kb on + strand at 82.166 kb on + strand at 82.821 kb on - strand at 82.832 kb on - strand at 82.849 kb on - strand at 82.868 kb on - strand at 82.971 kb on + strand at 82.985 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 60 mM remove 80,911 + -0.2 80,911 + +0.2 80,911 + +0.7 80,911 + -2.0 80,912 - +0.1 80,912 - +0.3 80,912 - -1.2 80,921 + -0.4 80,921 + -0.4 80,921 + +0.2 80,936 - -0.5 81,740 + -0.0 81,740 + -0.6 81,740 + -0.9 81,740 + +1.0 81,742 + +0.4 81,809 + +0.5 81,809 + +0.6 81,937 + PS417_00415 0.26 -1.3 82,159 + -2.9 82,159 + -0.3 82,159 + -0.7 82,166 + +0.3 82,821 - -0.1 82,832 - +0.9 82,849 - -0.9 82,868 - -1.3 82,971 + -0.5 82,985 + -0.0
Or see this region's nucleotide sequence