Experiment: LB_plus_SM_buffer with 6F2_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4727 and GFF4728 overlap by 4 nucleotides GFF4728 and GFF4729 overlap by 4 nucleotides GFF4729 and GFF4730 overlap by 10 nucleotides GFF4730 and GFF4731 are separated by 98 nucleotides
GFF4727 - Manganese ABC transporter, periplasmic-binding protein SitA, at 36,151 to 37,068
GFF4727
GFF4728 - Manganese ABC transporter, ATP-binding protein SitB, at 37,065 to 37,886
GFF4728
GFF4729 - Manganese ABC transporter, inner membrane permease protein SitC, at 37,883 to 38,743
GFF4729
GFF4730 - Manganese ABC transporter, inner membrane permease protein SitD, at 38,734 to 39,582
GFF4730
GFF4731 - Type III secretion injected virulence protein (YopP,YopJ, induces apoptosis, prevents cytokine induction, inhibits NFkb activation), at 39,681 to 40,547
GFF4731
Position (kb) 37
38
39 Strain fitness (log2 ratio) -2
-1
0
1 at 36.952 kb on + strand, within GFF4727 at 36.994 kb on + strand at 36.995 kb on - strand at 36.995 kb on - strand at 37.178 kb on - strand, within GFF4728 at 37.306 kb on - strand, within GFF4728 at 37.396 kb on + strand, within GFF4728 at 37.585 kb on + strand, within GFF4728 at 37.695 kb on - strand, within GFF4728 at 38.030 kb on - strand, within GFF4729 at 38.117 kb on - strand, within GFF4729 at 38.171 kb on - strand, within GFF4729 at 38.441 kb on - strand, within GFF4729 at 38.596 kb on + strand, within GFF4729 at 38.597 kb on - strand, within GFF4729 at 38.601 kb on - strand, within GFF4729 at 38.775 kb on + strand at 38.825 kb on + strand, within GFF4730 at 38.825 kb on + strand, within GFF4730 at 38.847 kb on - strand, within GFF4730 at 38.911 kb on + strand, within GFF4730 at 38.911 kb on + strand, within GFF4730 at 38.912 kb on - strand, within GFF4730 at 38.913 kb on + strand, within GFF4730 at 38.914 kb on - strand, within GFF4730 at 38.914 kb on - strand, within GFF4730 at 39.065 kb on - strand, within GFF4730 at 39.114 kb on + strand, within GFF4730 at 39.182 kb on + strand, within GFF4730 at 39.268 kb on + strand, within GFF4730 at 39.279 kb on - strand, within GFF4730 at 39.282 kb on + strand, within GFF4730 at 39.298 kb on - strand, within GFF4730 at 39.612 kb on - strand at 39.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.001875 MOI remove 36,952 + GFF4727 0.87 -0.1 36,994 + +0.4 36,995 - -0.8 36,995 - -0.1 37,178 - GFF4728 0.14 -0.7 37,306 - GFF4728 0.29 -0.0 37,396 + GFF4728 0.40 -0.3 37,585 + GFF4728 0.63 +0.2 37,695 - GFF4728 0.77 -0.9 38,030 - GFF4729 0.17 -0.1 38,117 - GFF4729 0.27 -0.5 38,171 - GFF4729 0.33 -0.4 38,441 - GFF4729 0.65 -0.1 38,596 + GFF4729 0.83 -2.0 38,597 - GFF4729 0.83 +0.4 38,601 - GFF4729 0.83 +0.0 38,775 + +0.1 38,825 + GFF4730 0.11 +0.1 38,825 + GFF4730 0.11 -0.4 38,847 - GFF4730 0.13 -0.3 38,911 + GFF4730 0.21 +0.1 38,911 + GFF4730 0.21 +0.1 38,912 - GFF4730 0.21 +0.5 38,913 + GFF4730 0.21 +0.2 38,914 - GFF4730 0.21 +0.8 38,914 - GFF4730 0.21 +0.5 39,065 - GFF4730 0.39 -0.0 39,114 + GFF4730 0.45 +0.4 39,182 + GFF4730 0.53 +0.2 39,268 + GFF4730 0.63 +0.1 39,279 - GFF4730 0.64 +0.6 39,282 + GFF4730 0.65 -0.4 39,298 - GFF4730 0.66 +0.1 39,612 - -0.6 39,612 - +0.3
Or see this region's nucleotide sequence