Experiment: LB_plus_SM_buffer with 6F2_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4706 and GFF4707 are separated by 145 nucleotides GFF4707 and GFF4708 overlap by 32 nucleotides GFF4708 and GFF4709 are separated by 97 nucleotides
GFF4706 - [NiFe] hydrogenase metallocenter assembly protein HypF, at 17,517 to 19,757
GFF4706
GFF4707 - Fe-S-cluster-containing hydrogenase components 2, at 19,903 to 20,448
GFF4707
GFF4708 - hypothetical protein, at 20,417 to 20,551
GFF4708
GFF4709 - Putative periplasmic or exported protein, at 20,649 to 21,458
GFF4709
Position (kb) 19
20
21 Strain fitness (log2 ratio) -1
0
1 at 18.909 kb on + strand, within GFF4706 at 18.913 kb on + strand, within GFF4706 at 18.914 kb on - strand, within GFF4706 at 18.930 kb on + strand, within GFF4706 at 19.308 kb on - strand, within GFF4706 at 19.574 kb on - strand at 19.637 kb on + strand at 19.760 kb on + strand at 19.760 kb on + strand at 19.816 kb on - strand at 19.885 kb on + strand at 20.020 kb on - strand, within GFF4707 at 20.126 kb on - strand, within GFF4707 at 20.319 kb on + strand, within GFF4707 at 20.485 kb on - strand, within GFF4708 at 20.487 kb on - strand, within GFF4708 at 20.487 kb on - strand, within GFF4708 at 20.751 kb on - strand, within GFF4709 at 20.754 kb on + strand, within GFF4709 at 20.778 kb on - strand, within GFF4709 at 20.855 kb on - strand, within GFF4709 at 20.856 kb on + strand, within GFF4709 at 21.022 kb on + strand, within GFF4709 at 21.023 kb on - strand, within GFF4709 at 21.023 kb on - strand, within GFF4709 at 21.286 kb on + strand, within GFF4709 at 21.400 kb on - strand at 21.437 kb on + strand at 21.438 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.001875 MOI remove 18,909 + GFF4706 0.62 -0.1 18,913 + GFF4706 0.62 -0.9 18,914 - GFF4706 0.62 +0.2 18,930 + GFF4706 0.63 +0.6 19,308 - GFF4706 0.80 -0.1 19,574 - +0.2 19,637 + -1.4 19,760 + -0.2 19,760 + -0.7 19,816 - +0.4 19,885 + +0.2 20,020 - GFF4707 0.21 -0.4 20,126 - GFF4707 0.41 +0.3 20,319 + GFF4707 0.76 -0.4 20,485 - GFF4708 0.50 +0.0 20,487 - GFF4708 0.52 -0.2 20,487 - GFF4708 0.52 -0.1 20,751 - GFF4709 0.13 -0.1 20,754 + GFF4709 0.13 -0.4 20,778 - GFF4709 0.16 -0.4 20,855 - GFF4709 0.25 -0.4 20,856 + GFF4709 0.26 +0.4 21,022 + GFF4709 0.46 +1.6 21,023 - GFF4709 0.46 +0.2 21,023 - GFF4709 0.46 +0.3 21,286 + GFF4709 0.79 +0.7 21,400 - +0.4 21,437 + -0.3 21,438 - +0.2
Or see this region's nucleotide sequence