Experiment: LB_plus_SM_buffer with 6F2_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4645 and GFF4646 are separated by 469 nucleotides GFF4646 and GFF4647 are separated by 15 nucleotides
GFF4645 - hypothetical protein, at 439,478 to 439,603
GFF4645
GFF4646 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 440,073 to 441,641
GFF4646
GFF4647 - Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-), at 441,657 to 442,796
GFF4647
Position (kb) 440
441
442 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 440.077 kb on + strand at 440.081 kb on + strand at 440.081 kb on + strand at 440.082 kb on - strand at 440.082 kb on - strand at 440.084 kb on - strand at 440.101 kb on + strand at 440.191 kb on - strand at 440.191 kb on - strand at 440.191 kb on - strand at 440.216 kb on + strand at 440.332 kb on - strand, within GFF4646 at 440.405 kb on - strand, within GFF4646 at 440.405 kb on - strand, within GFF4646 at 440.438 kb on + strand, within GFF4646 at 440.450 kb on + strand, within GFF4646 at 440.451 kb on - strand, within GFF4646 at 440.685 kb on + strand, within GFF4646 at 440.686 kb on - strand, within GFF4646 at 440.688 kb on + strand, within GFF4646 at 440.689 kb on - strand, within GFF4646 at 440.764 kb on + strand, within GFF4646 at 441.353 kb on - strand, within GFF4646 at 441.359 kb on + strand, within GFF4646 at 441.409 kb on - strand, within GFF4646 at 441.516 kb on + strand at 441.517 kb on - strand at 441.673 kb on + strand at 441.673 kb on + strand at 441.807 kb on - strand, within GFF4647 at 441.807 kb on - strand, within GFF4647 at 442.090 kb on + strand, within GFF4647 at 442.215 kb on + strand, within GFF4647 at 442.292 kb on - strand, within GFF4647 at 442.326 kb on + strand, within GFF4647 at 442.326 kb on + strand, within GFF4647 at 442.327 kb on - strand, within GFF4647 at 442.332 kb on - strand, within GFF4647 at 442.494 kb on - strand, within GFF4647 at 442.563 kb on - strand, within GFF4647
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.001875 MOI remove 440,077 + -3.2 440,081 + -0.4 440,081 + -1.5 440,082 - -1.8 440,082 - -2.9 440,084 - -2.7 440,101 + -2.5 440,191 - -1.8 440,191 - -0.4 440,191 - -1.7 440,216 + -1.7 440,332 - GFF4646 0.17 -3.0 440,405 - GFF4646 0.21 -2.6 440,405 - GFF4646 0.21 -2.3 440,438 + GFF4646 0.23 -3.0 440,450 + GFF4646 0.24 -1.6 440,451 - GFF4646 0.24 -3.4 440,685 + GFF4646 0.39 -2.2 440,686 - GFF4646 0.39 -3.0 440,688 + GFF4646 0.39 -1.2 440,689 - GFF4646 0.39 -2.3 440,764 + GFF4646 0.44 -1.8 441,353 - GFF4646 0.82 +0.1 441,359 + GFF4646 0.82 -4.2 441,409 - GFF4646 0.85 -2.9 441,516 + -2.8 441,517 - -2.2 441,673 + -3.4 441,673 + -2.4 441,807 - GFF4647 0.13 -0.7 441,807 - GFF4647 0.13 -2.2 442,090 + GFF4647 0.38 -1.9 442,215 + GFF4647 0.49 -4.4 442,292 - GFF4647 0.56 -1.9 442,326 + GFF4647 0.59 -3.2 442,326 + GFF4647 0.59 -1.7 442,327 - GFF4647 0.59 -3.0 442,332 - GFF4647 0.59 -1.2 442,494 - GFF4647 0.73 -1.6 442,563 - GFF4647 0.79 -1.0
Or see this region's nucleotide sequence