Experiment: LB_plus_SM_buffer with 6F2_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1962 and GFF1963 overlap by 4 nucleotides GFF1963 and GFF1964 are separated by 13 nucleotides GFF1964 and GFF1965 are separated by 131 nucleotides
GFF1962 - Phosphoglycerate transport system sensor protein PgtB (EC 2.7.3.-), at 154,888 to 156,894
GFF1962
GFF1963 - Phosphoglycerate transport regulatory protein PgtC, at 156,891 to 158,114
GFF1963
GFF1964 - FIG01047539: hypothetical protein, at 158,128 to 158,388
GFF1964
GFF1965 - Phosphoglycerate transporter protein PgtP, at 158,520 to 159,911
GFF1965
Position (kb) 156
157
158
159 Strain fitness (log2 ratio) -2
-1
0
1 at 155.935 kb on - strand, within GFF1962 at 155.941 kb on + strand, within GFF1962 at 155.942 kb on - strand, within GFF1962 at 155.980 kb on - strand, within GFF1962 at 156.112 kb on - strand, within GFF1962 at 156.279 kb on + strand, within GFF1962 at 156.280 kb on - strand, within GFF1962 at 156.281 kb on + strand, within GFF1962 at 156.282 kb on - strand, within GFF1962 at 156.283 kb on + strand, within GFF1962 at 156.284 kb on - strand, within GFF1962 at 156.284 kb on - strand, within GFF1962 at 156.492 kb on - strand, within GFF1962 at 156.613 kb on - strand, within GFF1962 at 156.625 kb on + strand, within GFF1962 at 156.637 kb on + strand, within GFF1962 at 156.842 kb on - strand at 156.984 kb on - strand at 157.050 kb on + strand, within GFF1963 at 157.051 kb on - strand, within GFF1963 at 157.167 kb on + strand, within GFF1963 at 157.323 kb on + strand, within GFF1963 at 157.324 kb on - strand, within GFF1963 at 157.324 kb on - strand, within GFF1963 at 157.324 kb on - strand, within GFF1963 at 157.327 kb on - strand, within GFF1963 at 157.421 kb on + strand, within GFF1963 at 157.457 kb on + strand, within GFF1963 at 157.459 kb on + strand, within GFF1963 at 157.460 kb on - strand, within GFF1963 at 157.930 kb on - strand, within GFF1963 at 157.953 kb on - strand, within GFF1963 at 158.063 kb on - strand at 158.103 kb on - strand at 158.586 kb on + strand at 158.602 kb on + strand at 158.735 kb on + strand, within GFF1965 at 158.736 kb on - strand, within GFF1965 at 158.742 kb on - strand, within GFF1965 at 158.767 kb on + strand, within GFF1965 at 158.942 kb on + strand, within GFF1965 at 159.005 kb on + strand, within GFF1965 at 159.087 kb on + strand, within GFF1965 at 159.089 kb on + strand, within GFF1965
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 0.001875 MOI remove 155,935 - GFF1962 0.52 -0.4 155,941 + GFF1962 0.52 +0.4 155,942 - GFF1962 0.53 +0.1 155,980 - GFF1962 0.54 -0.2 156,112 - GFF1962 0.61 -0.1 156,279 + GFF1962 0.69 +1.2 156,280 - GFF1962 0.69 -0.5 156,281 + GFF1962 0.69 +0.3 156,282 - GFF1962 0.69 +0.6 156,283 + GFF1962 0.70 -0.8 156,284 - GFF1962 0.70 +0.9 156,284 - GFF1962 0.70 -0.2 156,492 - GFF1962 0.80 -0.2 156,613 - GFF1962 0.86 +0.5 156,625 + GFF1962 0.87 +0.3 156,637 + GFF1962 0.87 +0.8 156,842 - +0.1 156,984 - -0.2 157,050 + GFF1963 0.13 -1.4 157,051 - GFF1963 0.13 -0.3 157,167 + GFF1963 0.23 -0.2 157,323 + GFF1963 0.35 -2.7 157,324 - GFF1963 0.35 +0.5 157,324 - GFF1963 0.35 -0.5 157,324 - GFF1963 0.35 +0.3 157,327 - GFF1963 0.36 -0.4 157,421 + GFF1963 0.43 -0.0 157,457 + GFF1963 0.46 -0.3 157,459 + GFF1963 0.46 +0.0 157,460 - GFF1963 0.46 -0.2 157,930 - GFF1963 0.85 -0.2 157,953 - GFF1963 0.87 +0.4 158,063 - +0.3 158,103 - -0.2 158,586 + +0.0 158,602 + -0.1 158,735 + GFF1965 0.15 -0.1 158,736 - GFF1965 0.16 -0.1 158,742 - GFF1965 0.16 -0.5 158,767 + GFF1965 0.18 +0.8 158,942 + GFF1965 0.30 +0.5 159,005 + GFF1965 0.35 +0.1 159,087 + GFF1965 0.41 -0.0 159,089 + GFF1965 0.41 -0.5
Or see this region's nucleotide sequence